A F Vanin, G B Men'shikov, I A Moroz, P I Mordvintsev, V A Serezhenkov, V S Repin, D Sh Burbaev
{"title":"[The source of the iron binding with nitric oxide in activated macrophages].","authors":"A F Vanin, G B Men'shikov, I A Moroz, P I Mordvintsev, V A Serezhenkov, V S Repin, D Sh Burbaev","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>No decrease in iron-sulphur centers was found in cultured macrophage cells (J774) after the treatment with nitric oxide (10(-7) M NO/10(7) cells) during 5 min. The center content was controlled by the electron spin resonance (ESR) method. The macrophages pretreated with dithionite + methyl viologen showed the formation of dinitrosyl iron complexes (DNIC) with a characteristic ESR signal at g approximately 2.03. The data suggest that loosely bound nonheme iron (free iron) mostly contributes to the formation of these complexes. Iron from iron-containing proteins does not release from these centers under the direct action of nitric oxide. The iron-sulphur centers can be destroyed by the products of nitric oxide oxidation (NO2, N2O3, etc.) as oxidizing and acid agents.</p>","PeriodicalId":14529,"journal":{"name":"Izvestiia Akademii nauk SSSR. Seriia biologicheskaia","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1991-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Izvestiia Akademii nauk SSSR. Seriia biologicheskaia","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
No decrease in iron-sulphur centers was found in cultured macrophage cells (J774) after the treatment with nitric oxide (10(-7) M NO/10(7) cells) during 5 min. The center content was controlled by the electron spin resonance (ESR) method. The macrophages pretreated with dithionite + methyl viologen showed the formation of dinitrosyl iron complexes (DNIC) with a characteristic ESR signal at g approximately 2.03. The data suggest that loosely bound nonheme iron (free iron) mostly contributes to the formation of these complexes. Iron from iron-containing proteins does not release from these centers under the direct action of nitric oxide. The iron-sulphur centers can be destroyed by the products of nitric oxide oxidation (NO2, N2O3, etc.) as oxidizing and acid agents.
一氧化氮(10(-7)M No /10(7) cells)处理5 min后,培养的巨噬细胞(J774)铁硫中心未见减少,中心含量由电子自旋共振(ESR)法控制。经二亚硝酸盐+甲基紫胶预处理的巨噬细胞形成二硝基铁配合物(DNIC),其特征ESR信号g约为2.03。数据表明,松散结合的非血红素铁(游离铁)主要有助于这些复合物的形成。含铁蛋白中的铁在一氧化氮的直接作用下不会从这些中心释放出来。一氧化氮氧化产物(NO2、N2O3等)作为氧化剂和酸剂可破坏铁硫中心。