Pyocyanin biomarker imprinted polydopamine for rapid and simple indirect detection of Pseudomonas aeruginosa

Abstract

This paper reports the first successful preparation of pyocyanin (PYO)-imprinted polydopamine-modified glassy carbon electrodes (PDA-GCE) using potentiodynamic electropolymerization. The molecularly imprinted PDA films showed an enhanced current response to pyocyanin than non-imprinted polydopamine (NIP) on GCE. The MIP-GCE sensor exhibited improved sensitivity, offering a linear range of 10 µM to 100 µM (r2 = 0.993), with a low limit of detection (6.45 µM) and limit of quantitation (21.5 µM). The MIP-PDA/GCE is a sensitive, selective electrochemical sensor for PYO. A pyocyanin (PYO)-imprinted polydopamine (MIP-PDA) was prepared for the first time. The MIP-PDA on glassy carbon is more sensitive to PYO than non-imprinted PDA. The MIP-PDA on glassy carbon is a sensitive, selective electrochemical sensor for PYO.