Effects of Ammonia Nitrogen, Nitrite and pH on Embryo In Vitro Incubation of Red Crayfish

Jianguo Wang, Gang Li, Yongjun Bao
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Abstract

As an important economic aquatic animal, red crayfish’s reproduction and incubation can affect its breeding efficiency. With aquaculture developing, the study of factors affecting aquaculture species growth has gradually become an important research direction. To study the effects of ammonia nitrogen, nitrite and pH on red crayfish embryos, the In Vitro incubation experiment of red crayfish embryos was conducted. The residual module was used to design the embryo counting method of red crayfish. And different ammonia nitrogen, nitrite and PH gradients were set to analyze the hatching rate and emergence rate of red crayfish’s embryo development.When conducting research method performance testing, the training loss value of the research method decreased to 84 after 200 iterations. On the 6th day in an ammonia nitrogen environment, the number of water microbiota was only 4.74 M. The emergence rate of red crayfish decreases to a lower level when the ammonia nitrogen concentration is above 0.6 mg/L. The emergence rate of red crayfish decreased with the increase of nitrite concentration, and decreased to 0 when the nitrite concentration reached 1.25 mg/L. When the pH value of water body is 8.1, the emergence rate of red crayfish reaches the highest value of 68.92%. Ammonia nitrogen and nitrite concentration will inhibit the In Vitro hatching of crayfish embryos, and the appropriate PH value can improve the emergence rate of crayfish hatching In Vitro.
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氨氮、亚硝酸盐和 pH 值对红螯虾胚胎体外孵化的影响
作为一种重要的经济水生动物,红螯虾的繁殖和孵化影响其繁殖效率。随着水产养殖的发展,对水产养殖物种生长影响因素的研究逐渐成为一个重要的研究方向。为研究氨氮、亚硝酸盐和pH对红螯虾胚胎的影响,进行了红螯虾胚胎体外培养实验。利用残差模块设计了红螯虾胚胎计数方法。设置不同的氨氮、亚硝酸盐和PH梯度,分析红螯虾胚胎发育的孵化率和出苗率。在进行研究方法性能测试时,经过200次迭代,研究方法的训练损失值下降到84。在氨氮环境下第6天,水体微生物群数量仅为4.74 m,当氨氮浓度高于0.6 mg/L时,红螯虾的出苗率下降到较低水平。随着亚硝酸盐浓度的增加,红螯虾的出苗率降低,当亚硝酸盐浓度达到1.25 mg/L时,红螯虾的出苗率降至0。当水体pH值为8.1时,红螯虾出苗率最高,达68.92%。氨氮和亚硝酸盐浓度会抑制小龙虾胚胎的体外孵化,适当的PH值可以提高小龙虾体外孵化的出苗率。
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
自引率
0.00%
发文量
60
审稿时长
6 months
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