Physiological and Photosynthetic Characteristics of Pumpkin Seedlings Cultivated Under Low-Light Stress

Yanfang Jiang, Zefa Liu, Hui Liu, M. Yusop, Shamsiah Abdullah
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Abstract

Low-light stress is known to have a significant impact on pumpkin yield in greenhouse conditions, it is crucial to understand the characteristics of low-light tolerance and identify resistant germplasm resources. In this study, four pumpkins (Cucurbita moschata) varieties were used, including three inbred lines (10–32, NY-1, and JB-1) and one hybrid line (NY-1×JB-1). Various low-light treatments (T0, no shade; T40, 40% shading; T80, 80% shading) were set, to explore their effects on morphology, photosynthetic characteristics, osmotic regulation substance content, and antioxidant enzyme activity in pumpkin seedling leaves. In the results, seedlings of inbred lines 10–32 and JB-1, as well as hybrid NY-1×JB-1, exhibited low-light resistance with shading rate increases. The maximum photochemical quantum yield of photosystem II (Fv/Fm) of all varieties decreased under low-light stress. Catalase (CAT) activity initially increased and then decreased, while superoxide dismutase (SOD) activity showed the opposite trend. Peroxidase (POD) activity gradually increased in 10–32, JB-1, and NY-1×JB-1, with JB-1 showing a significant increase at T80, and NY-1 displaying an initial decrease followed by an increase. Noteablely, the soluble proline (Pro) content decreased in 10–32, NY-1, and NY-1×JB-1. Significant differences in malondialdehyde (MDA) content were observed among the seedlings of four varieties. The MDA contents increased in 10–32 and JB-1 under T0 and T40, while showed no significant change in NY-1. The MDA content at T80 significantly decreased in NY-1, and first decreased and then significantly increased in NY-1×B-1. The soluble sugar content in 10–32 remained unchanged between T0 and T40, but significantly increased at T80. While in JB-1 and NY-1, it initially increased then decreased with increasing shading rate. The trend in NY-1×JB-1 was opposite to that of JB-1 and NY-1, with initially decreased followed by a significant increase. In conclusion, morphological characteristics associated with low-light tolerance can serve as reference indices for breeding low-light tolerance pumpkins. These parameters can be used as reference indices to assess pumpkin resistance to low-light stress.
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低光照胁迫下栽培南瓜幼苗的生理和光合特性
众所周知,在温室条件下,弱光胁迫对南瓜产量有显著影响,因此了解耐弱光性的特征并确定抗性种质资源至关重要。本研究使用了四个南瓜(Cucurbita moschata)品种,包括三个近交系(10-32、NY-1 和 JB-1)和一个杂交系(NY-1×JB-1)。设置不同的弱光处理(T0,不遮光;T40,遮光40%;T80,遮光80%),探讨其对南瓜幼苗叶片形态、光合特性、渗透调节物质含量和抗氧化酶活性的影响。结果表明,随着遮光率的增加,近交系 10-32 和 JB-1 以及杂交种 NY-1×JB-1 的幼苗表现出抗弱光性。在弱光胁迫下,所有品种的光系统 II 最大光化量子产率(Fv/Fm)都有所下降。过氧化氢酶(CAT)活性先升高后降低,而超氧化物歧化酶(SOD)活性则呈现相反的趋势。过氧化物酶(POD)活性在 10-32、JB-1 和 NY-1×JB-1 中逐渐增加,其中 JB-1 在 T80 时显著增加,NY-1 则先降后升。值得注意的是,可溶性脯氨酸(Pro)含量在 10-32、NY-1 和 NY-1×JB-1 中均有所下降。在四个品种的幼苗中,丙二醛(MDA)含量存在显著差异。在 T0 和 T40 期,10-32 和 JB-1 的 MDA 含量增加,而 NY-1 的 MDA 含量没有显著变化。在 T80 时,NY-1 的 MDA 含量明显下降,NY-1×B-1 的 MDA 含量先下降后明显上升。10-32 中的可溶性糖含量在 T0 和 T40 之间保持不变,但在 T80 时明显增加。而在 JB-1 和 NY-1 中,随着遮光率的增加,可溶性糖含量先增加后减少。NY-1×JB-1的趋势与JB-1和NY-1相反,最初下降,随后显著上升。 总之,与耐弱光性相关的形态特征可作为耐弱光性南瓜育种的参考指标。这些参数可作为评估南瓜抗弱光胁迫能力的参考指标。
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来源期刊
Journal of Biobased Materials and Bioenergy
Journal of Biobased Materials and Bioenergy 工程技术-材料科学:生物材料
自引率
0.00%
发文量
60
审稿时长
6 months
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