In vitro assessment of antibacterial and antiviral activity of three copper products after 200 rounds of simulated use

IF 4.1 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biometals Pub Date : 2023-12-22 DOI:10.1007/s10534-023-00572-z
Marthe K. Charles, Teresa C. Williams, Davood Nakhaie, Tracey Woznow, Billie Velapatino, Ana C. Lorenzo-Leal, Horacio Bach, Elizabeth A. Bryce, Edouard Asselin
{"title":"In vitro assessment of antibacterial and antiviral activity of three copper products after 200 rounds of simulated use","authors":"Marthe K. Charles,&nbsp;Teresa C. Williams,&nbsp;Davood Nakhaie,&nbsp;Tracey Woznow,&nbsp;Billie Velapatino,&nbsp;Ana C. Lorenzo-Leal,&nbsp;Horacio Bach,&nbsp;Elizabeth A. Bryce,&nbsp;Edouard Asselin","doi":"10.1007/s10534-023-00572-z","DOIUrl":null,"url":null,"abstract":"<div><p>Copper has well-documented antibacterial effects but few have evaluated it after prolonged use and against bacteria and viruses. Coupons from three copper formulations (solid, thermal coating, and decal applications) and carbon steel controls were subjected to 200 rounds simulated cleaning using a Wiperator™ and either an accelerated hydrogen peroxide, quaternary ammonium, or artificial sweat products. Antibacterial activity against <i>S. aureus</i> and <i>P. aeruginosa</i> was then evaluated using a modified Environmental Protection Agency protocol. Antiviral activity against coronavirus (229E) and norovirus (MNV-1) surrogates was assessed using the TCID<sub>50</sub> method. Results were compared to untreated control coupons. One hour after inoculation, <i>S. aureus</i> exhibited a difference in log kill of 1.16 to 4.87 and <i>P. aeruginosa</i> a log kill difference of 3.39–5.23 (dependent upon copper product and disinfectant) compared to carbon steel. MNV-1 demonstrated an 87–99% reduction on each copper surfaces at 1 h and 99% reduction at 2 h compared to carbon steel. Similarly, coronavirus 229E exhibited a 97–99% reduction after 1 h and 90–99% after 2 h. Simulated use with artificial sweat did not hinder the antiviral nor the antibacterial activity of Cu surfaces. Self-sanitizing copper surfaces maintained antibacterial and antiviral activity after 200 rounds of simulated cleaning. </p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":491,"journal":{"name":"Biometals","volume":null,"pages":null},"PeriodicalIF":4.1000,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11255078/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biometals","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10534-023-00572-z","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Copper has well-documented antibacterial effects but few have evaluated it after prolonged use and against bacteria and viruses. Coupons from three copper formulations (solid, thermal coating, and decal applications) and carbon steel controls were subjected to 200 rounds simulated cleaning using a Wiperator™ and either an accelerated hydrogen peroxide, quaternary ammonium, or artificial sweat products. Antibacterial activity against S. aureus and P. aeruginosa was then evaluated using a modified Environmental Protection Agency protocol. Antiviral activity against coronavirus (229E) and norovirus (MNV-1) surrogates was assessed using the TCID50 method. Results were compared to untreated control coupons. One hour after inoculation, S. aureus exhibited a difference in log kill of 1.16 to 4.87 and P. aeruginosa a log kill difference of 3.39–5.23 (dependent upon copper product and disinfectant) compared to carbon steel. MNV-1 demonstrated an 87–99% reduction on each copper surfaces at 1 h and 99% reduction at 2 h compared to carbon steel. Similarly, coronavirus 229E exhibited a 97–99% reduction after 1 h and 90–99% after 2 h. Simulated use with artificial sweat did not hinder the antiviral nor the antibacterial activity of Cu surfaces. Self-sanitizing copper surfaces maintained antibacterial and antiviral activity after 200 rounds of simulated cleaning. 

Graphical abstract

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
在模拟使用 200 次后,对三种铜产品的抗菌和抗病毒活性进行体外评估。
铜的抗菌效果是有据可查的,但很少有人对其长期使用后的细菌和病毒效果进行评估。使用 Wiperator™ 和加速过氧化氢、季铵盐或人工汗液产品对三种铜配方(固体、热涂层和贴花应用)的试样和碳钢对照进行了 200 次模拟清洗。然后使用修改后的环境保护局方案对金黄色葡萄球菌和绿脓杆菌的抗菌活性进行评估。对冠状病毒(229E)和诺如病毒(MNV-1)代用品的抗病毒活性采用 TCID50 法进行评估。结果与未经处理的对照试样进行了比较。接种一小时后,与碳钢相比,金黄色葡萄球菌的杀灭对数相差 1.16 到 4.87,铜绿假单胞菌的杀灭对数相差 3.39 到 5.23(取决于铜产品和消毒剂)。与碳钢相比,MNV-1 在每个铜表面 1 小时的杀灭率为 87%-99%,2 小时的杀灭率为 99%。同样,冠状病毒 229E 在 1 小时后的抑制率为 97%-99%,2 小时后为 90%-99%。模拟使用人工汗液不会妨碍铜表面的抗病毒或抗菌活性。经过 200 次模拟清洗后,自消毒铜表面仍能保持抗菌和抗病毒活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Biometals
Biometals 生物-生化与分子生物学
CiteScore
5.90
自引率
8.60%
发文量
111
审稿时长
3 months
期刊介绍: BioMetals is the only established journal to feature the important role of metal ions in chemistry, biology, biochemistry, environmental science, and medicine. BioMetals is an international, multidisciplinary journal singularly devoted to the rapid publication of the fundamental advances of both basic and applied research in this field. BioMetals offers a forum for innovative research and clinical results on the structure and function of: - metal ions - metal chelates, - siderophores, - metal-containing proteins - biominerals in all biosystems. - BioMetals rapidly publishes original articles and reviews. BioMetals is a journal for metals researchers who practice in medicine, biochemistry, pharmacology, toxicology, microbiology, cell biology, chemistry, and plant physiology who are based academic, industrial and government laboratories.
期刊最新文献
Metallic nanoparticles: a promising novel therapeutic tool against antimicrobial resistance and spread of superbugs. Copper biomarkers and their relationship with dyslipidemia in women with obesity. Effect of co-exposure to multiple metals (Pb, Cd, Cr, Hg, Fe, Mn and Ni) and metalloid (As) on liver function in Swiss albino mice. A new spectrophotometric method for measuring ceruloplasmin ferroxidase activity: an innovative approach. The arylbipyridine platinum (II) complex increases the level of ROS and induces lipid peroxidation in glioblastoma cells.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1