THE COMPARISON OF SIDEROPHORES CONTENT IN BACTERIA ISOLATED FROM BLACK SEA MUSSELS

Abstract

Aim. This research was carried out for determination of ability to produce siderophores by strains of Pseudomonas aeruginosa and Bacillus sp. isolated from Black Sea mussels and for studying characteristics of their synthesis at mono- and co-cultivation of researched strains. Materials and methods. In the study we used four strains of Pseudomonas aeruginosa and two strains of Bacillus sp. Monocultivation and co-cultivation were carried out with these strains on a LB medium. CAS (chrome azurol S) analysis was used to determine the content of synthesized siderophores, the measurement was carried out in spectrophotometer SmartSpec Plus at 630 nm. Conclusions. This study showed that marine strains of P. aeruginosa can produce more siderophores than marine strains of Bacillus sp. At monocultivation, strain P. aeruginosa М1 was able to produce the largest amount of siderophores with value of SU (siderophores units) 65 ± 4% and the smallest one strain B. atrophaeus МН4 with value of SU 21 ± 1%. Co-cultivation provides an increase in production of siderophores in each strain, that is the result of special interactions between different microorganisms. And through it, the combination B. subtilis MC3+P. aeruginosa M1 demonstrated the highest content of siderophores with value of SU 81 ± 6%, the lowest content was shown by combination B. atrophaeus МН4+P. aeruginosa M3 with value of SU 41 ± 4%. And such results showed that co-cultivation is the useful method for obtaining more content of siderophores from already famous strains.