Pub Date : 2023-12-20DOI: 10.18524/2307-4663.2023.3(59).287367
S. A. Martynenko, M. O. Finogenova, A. Semenets, M. Galkin
Aim. This research was carried out for determination of ability to produce siderophores by strains of Pseudomonas aeruginosa and Bacillus sp. isolated from Black Sea mussels and for studying characteristics of their synthesis at mono- and co-cultivation of researched strains. Materials and methods. In the study we used four strains of Pseudomonas aeruginosa and two strains of Bacillus sp. Monocultivation and co-cultivation were carried out with these strains on a LB medium. CAS (chrome azurol S) analysis was used to determine the content of synthesized siderophores, the measurement was carried out in spectrophotometer SmartSpec Plus at 630 nm. Conclusions. This study showed that marine strains of P. aeruginosa can produce more siderophores than marine strains of Bacillus sp. At monocultivation, strain P. aeruginosa М1 was able to produce the largest amount of siderophores with value of SU (siderophores units) 65 ± 4% and the smallest one strain B. atrophaeus МН4 with value of SU 21 ± 1%. Co-cultivation provides an increase in production of siderophores in each strain, that is the result of special interactions between different microorganisms. And through it, the combination B. subtilis MC3+P. aeruginosa M1 demonstrated the highest content of siderophores with value of SU 81 ± 6%, the lowest content was shown by combination B. atrophaeus МН4+P. aeruginosa M3 with value of SU 41 ± 4%. And such results showed that co-cultivation is the useful method for obtaining more content of siderophores from already famous strains.
研究目的本研究旨在确定从黑海贻贝中分离出来的铜绿假单胞菌和芽孢杆菌菌株产生嗜苷酸盐的能力，并研究这些菌株在单一培养和联合培养时合成嗜苷酸盐的特点。材料和方法。在研究中，我们使用了四株铜绿假单胞菌和两株芽孢杆菌。CAS（铬唑啉 S）分析用于确定合成嗜苷酸盐的含量，测量在分光光度计 SmartSpec Plus 上进行，波长为 630 nm。结论该研究表明，海洋铜绿微囊藻菌株比海洋芽孢杆菌菌株能产生更多的嗜苷酸盐。在单一培养条件下，菌株铜绿微囊藻М1能产生最多的嗜苷酸盐，SU（嗜苷酸盐单位）值为 65 ± 4%，而菌株芽孢杆菌МН4产生的嗜苷酸盐最少，SU 值为 21 ± 1%。联合培养能提高每个菌株嗜苷酸盐的产量，这是不同微生物之间特殊相互作用的结果。通过联合培养，枯草芽孢杆菌 MC3+ 铜绿微囊藻 M1 的嗜苷酸含量最高，为 SU 81 ± 6%，而阿特罗非斯芽孢杆菌 МН4+ 铜绿微囊藻 M3 的嗜苷酸含量最低，为 SU 41 ± 4%。这些结果表明，联合培养是从著名菌株中获得更多嗜苷酸含量的有效方法。
{"title":"THE COMPARISON OF SIDEROPHORES CONTENT IN BACTERIA ISOLATED FROM BLACK SEA MUSSELS","authors":"S. A. Martynenko, M. O. Finogenova, A. Semenets, M. Galkin","doi":"10.18524/2307-4663.2023.3(59).287367","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.3(59).287367","url":null,"abstract":"Aim. This research was carried out for determination of ability to produce siderophores by strains of Pseudomonas aeruginosa and Bacillus sp. isolated from Black Sea mussels and for studying characteristics of their synthesis at mono- and co-cultivation of researched strains. Materials and methods. In the study we used four strains of Pseudomonas aeruginosa and two strains of Bacillus sp. Monocultivation and co-cultivation were carried out with these strains on a LB medium. CAS (chrome azurol S) analysis was used to determine the content of synthesized siderophores, the measurement was carried out in spectrophotometer SmartSpec Plus at 630 nm. Conclusions. This study showed that marine strains of P. aeruginosa can produce more siderophores than marine strains of Bacillus sp. At monocultivation, strain P. aeruginosa М1 was able to produce the largest amount of siderophores with value of SU (siderophores units) 65 ± 4% and the smallest one strain B. atrophaeus МН4 with value of SU 21 ± 1%. Co-cultivation provides an increase in production of siderophores in each strain, that is the result of special interactions between different microorganisms. And through it, the combination B. subtilis MC3+P. aeruginosa M1 demonstrated the highest content of siderophores with value of SU 81 ± 6%, the lowest content was shown by combination B. atrophaeus МН4+P. aeruginosa M3 with value of SU 41 ± 4%. And such results showed that co-cultivation is the useful method for obtaining more content of siderophores from already famous strains.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"2 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138954943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.18524/2307-4663.2023.3(59).292392
S. I. Burykina, S. P. Uzhevska, N. Pylyak
Aim – Studing of methods for determining the effectiveness of Nematophagin BT against potato stem nematode in the laboratory. Materials and methods. For the experiment, potato tubers with signs of damage by the stem nematode Ditylenchus destructor Thorne, 1945 were selected. The method of treatment of cut potato tubers with the preparation was used. It was compared with the method of treatment of whole tubers in the laboratory. The effect of the microbiological nematicidal preparation (Nematofagin BT) developed by ITI Biotechnology of NAAS was determined. The effectiveness of the microbiological preparation of nematicidal action (Nematofagin BT), developed by ITI Biotechnology of NAAS, was determined. The active ingredient of the drug is a microscopic fungus-predator Arthrobotrys oligospora strain 12, the initial culture of which is deposited in the collection of microorganism cultures of the D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine F100047. Results. The methods of laboratory evaluation of the nematicidal effect of the biological product against potato stem nematode are described. In laboratory conditions, to determine the effectiveness of the drug, it is more informative to use cut tubers that have been infected with stem nematode. This method requires much more time to set up, but the bioagent has bigger accessibility to the pest. The use of whole tubers is less labor-intensive, but there is a possibility of using uninfected material in the experiment. The expediency of using these methods in laboratory conditions for the selection of active strains and determination of their effectiveness is shown. Conclusion. The method of nematicidal treatment of cut tubers with established infection of stem nematode is more informative than the use of whole tubers. It is advisable to analyze for damage to potatoes by nematodes when using pieces of affected potatoes from 3 to 7 days after treatment with a 3% solution of the biological product with an exposure of 5 hours. In laboratory conditions, both methods of treatment of cut and whole tubers with the preparation can be used to test the effect of Nematophagin BT on potato stem nematode. Nematophagin BT showed nematicidal activity against potato stem nematode in laboratory conditions.
{"title":"METHOD OF ASSESSING THE NEMATOCIDAL EFFECTIVENESS OF A BIOPREPA-RATION BASED ON PREDATORY MICROMYCETE AGAINST THE POTATO STEM NEMATODE","authors":"S. I. Burykina, S. P. Uzhevska, N. Pylyak","doi":"10.18524/2307-4663.2023.3(59).292392","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.3(59).292392","url":null,"abstract":"Aim – Studing of methods for determining the effectiveness of Nematophagin BT against potato stem nematode in the laboratory. Materials and methods. For the experiment, potato tubers with signs of damage by the stem nematode Ditylenchus destructor Thorne, 1945 were selected. The method of treatment of cut potato tubers with the preparation was used. It was compared with the method of treatment of whole tubers in the laboratory. The effect of the microbiological nematicidal preparation (Nematofagin BT) developed by ITI Biotechnology of NAAS was determined. The effectiveness of the microbiological preparation of nematicidal action (Nematofagin BT), developed by ITI Biotechnology of NAAS, was determined. The active ingredient of the drug is a microscopic fungus-predator Arthrobotrys oligospora strain 12, the initial culture of which is deposited in the collection of microorganism cultures of the D.K. Zabolotny Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine F100047. Results. The methods of laboratory evaluation of the nematicidal effect of the biological product against potato stem nematode are described. In laboratory conditions, to determine the effectiveness of the drug, it is more informative to use cut tubers that have been infected with stem nematode. This method requires much more time to set up, but the bioagent has bigger accessibility to the pest. The use of whole tubers is less labor-intensive, but there is a possibility of using uninfected material in the experiment. The expediency of using these methods in laboratory conditions for the selection of active strains and determination of their effectiveness is shown. Conclusion. The method of nematicidal treatment of cut tubers with established infection of stem nematode is more informative than the use of whole tubers. It is advisable to analyze for damage to potatoes by nematodes when using pieces of affected potatoes from 3 to 7 days after treatment with a 3% solution of the biological product with an exposure of 5 hours. In laboratory conditions, both methods of treatment of cut and whole tubers with the preparation can be used to test the effect of Nematophagin BT on potato stem nematode. Nematophagin BT showed nematicidal activity against potato stem nematode in laboratory conditions.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"7 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138956104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.18524/2307-4663.2023.3(59).286968
N. Pylyak, L. Loban
The Engineering and Technological Institute "Biotechnic" of the National Academy of Sciences of Ukraine has collected and preserve the viable collection of microorganisms, which was formed on the basis of expediency and efficiency in the biologization of agriculture. The microbial gene pool of the Collection bacterial and fungal strains of microorganisms that are used in biotechnologies for the production of plant protection products and their quality control. Microbiopreparations with fungicidal, entomocidal, phosphate-mobilizing, nematicidal, cellulolytic, and growth-stimulating properties have been created on the basis of collection microorganisms. The Collection also preserve strains that influenced on the atmospheric nitrogen fixation processes. The great interest present the microorganisms isolated from natural sources of different regions of Ukraine (author's strains), most of which are in studying and accumulating information about them. To determine the biological activity of fungicides, the Collection supports fungal test objects that are the causative agents of diseases of vegetable, grain, fruit and berry crops. The Collection of non-pathogenic microorganisms was created for working in the field of plant protection, as well as for teaching students of biology, biotechnologies, and agronomists. The collection is specialized, therefore the preservation of such unique objects is a priority task, since the losts of collection microorganisms can have negative consequences for the further improvement and development of biotechnology, in particular, the production of microbiological preparations for plant protection using the potential of the Collection. Taking into account all of the above, on November 4, 2022, the collection was entered into the State Register of Scientific Objects, which constitute national property (Resolution of the Cabinet of Ukraine of November 4, 2022, No. 1243).
{"title":"A COLLECTION OF INDUSTRIALLY VALUABLE CULTURES OF MICROORGANISMS FOR AGRICULTURAL BIOLOGY","authors":"N. Pylyak, L. Loban","doi":"10.18524/2307-4663.2023.3(59).286968","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.3(59).286968","url":null,"abstract":"The Engineering and Technological Institute \"Biotechnic\" of the National Academy of Sciences of Ukraine has collected and preserve the viable collection of microorganisms, which was formed on the basis of expediency and efficiency in the biologization of agriculture. The microbial gene pool of the Collection bacterial and fungal strains of microorganisms that are used in biotechnologies for the production of plant protection products and their quality control. Microbiopreparations with fungicidal, entomocidal, phosphate-mobilizing, nematicidal, cellulolytic, and growth-stimulating properties have been created on the basis of collection microorganisms. The Collection also preserve strains that influenced on the atmospheric nitrogen fixation processes. The great interest present the microorganisms isolated from natural sources of different regions of Ukraine (author's strains), most of which are in studying and accumulating information about them. To determine the biological activity of fungicides, the Collection supports fungal test objects that are the causative agents of diseases of vegetable, grain, fruit and berry crops. The Collection of non-pathogenic microorganisms was created for working in the field of plant protection, as well as for teaching students of biology, biotechnologies, and agronomists. The collection is specialized, therefore the preservation of such unique objects is a priority task, since the losts of collection microorganisms can have negative consequences for the further improvement and development of biotechnology, in particular, the production of microbiological preparations for plant protection using the potential of the Collection. Taking into account all of the above, on November 4, 2022, the collection was entered into the State Register of Scientific Objects, which constitute national property (Resolution of the Cabinet of Ukraine of November 4, 2022, No. 1243).","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"31 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138955670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.18524/2307-4663.2023.3(59).293001
О. V. Andriushchenko, I. V. Strashnova
Biological features, pathogenesis, methods of detection, identification and control of fungi of the genus Fusarium, capable of causing diseases in various types of plants, in barley and wheat. Information is provided about the main mycotoxins synthesized by fusaria, as well as ways to reduce their concentration in the affected grain. The need for the development and implementation of biological methods for controlling fusarium pathogens is noted.
{"title":"CHARACTERISTICS OF REPRESENTATIVES OF THE GENUS FUSARIUM CAUSING CEREAL CROPS DISEASES","authors":"О. V. Andriushchenko, I. V. Strashnova","doi":"10.18524/2307-4663.2023.3(59).293001","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.3(59).293001","url":null,"abstract":"Biological features, pathogenesis, methods of detection, identification and control of fungi of the genus Fusarium, capable of causing diseases in various types of plants, in barley and wheat. Information is provided about the main mycotoxins synthesized by fusaria, as well as ways to reduce their concentration in the affected grain. The need for the development and implementation of biological methods for controlling fusarium pathogens is noted.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"106 21","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-12-20DOI: 10.18524/2307-4663.2023.3(59).287048
A. Merlich, I. I. Kimurzhyi, R. R. Kovalchuk, M. V. Shutylo, V. Ivanytsia
Aim. Determination of the presence of antimycotic activity and its level in thirteen lactobacteria isolates from Black Sea water and mussels. Methods. Antimycotic activity of lactic acid bacteria (LAB) isolates was tested by agar diffusion method in 24-well plates. To determine character of action the plates were incubated for 7 days at 25 °C. Results. For the first time antimycotic activity of marine LAB from Odesa coast was estimated. The majority of the tested isolates of lactobateria exhibited antimycotic activity of high level completely inhibiting (in 100%) as mycelium growth as well as spore formation of Penicillium expansum UKM F-575 and Aspergillus niger UKM F-16706 on second day of the study. Four isolates (M.5.1, M.5.2, M.7.1, M.7.2) showed lower antimycotic activity (from 0 to 75%). Eight LAB isolates from seawater (W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4) and one isolate from mussel liquor (M4.1) completely inhibited mycelial growth and sporulation of P. expansum UКМ F-575 and A. niger UКМ F-16706 even within seven days indicating fungicidal character of action. Conclusions. The most of tested LAB isolates from Black Sea exhibited high antimycotic activity against P. expansum UКМ F-575 and A. niger UКМ F-16706 indicator strains. The most active lactobacteria isolates were W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4 isolated from Black Sea water and M4.1 – from mussel liquor. The character of the revealed antimycotic activity was determined as fungicidal.
{"title":"ANTIMYCOTIC ACTIVITY OF THE ISOLATES OF LACTOBACTERIA FROM WA-TER AND MUSSELS OF BLACK SEA","authors":"A. Merlich, I. I. Kimurzhyi, R. R. Kovalchuk, M. V. Shutylo, V. Ivanytsia","doi":"10.18524/2307-4663.2023.3(59).287048","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.3(59).287048","url":null,"abstract":"Aim. Determination of the presence of antimycotic activity and its level in thirteen lactobacteria isolates from Black Sea water and mussels. Methods. Antimycotic activity of lactic acid bacteria (LAB) isolates was tested by agar diffusion method in 24-well plates. To determine character of action the plates were incubated for 7 days at 25 °C. Results. For the first time antimycotic activity of marine LAB from Odesa coast was estimated. The majority of the tested isolates of lactobateria exhibited antimycotic activity of high level completely inhibiting (in 100%) as mycelium growth as well as spore formation of Penicillium expansum UKM F-575 and Aspergillus niger UKM F-16706 on second day of the study. Four isolates (M.5.1, M.5.2, M.7.1, M.7.2) showed lower antimycotic activity (from 0 to 75%). Eight LAB isolates from seawater (W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4) and one isolate from mussel liquor (M4.1) completely inhibited mycelial growth and sporulation of P. expansum UКМ F-575 and A. niger UКМ F-16706 even within seven days indicating fungicidal character of action. Conclusions. The most of tested LAB isolates from Black Sea exhibited high antimycotic activity against P. expansum UКМ F-575 and A. niger UКМ F-16706 indicator strains. The most active lactobacteria isolates were W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4 isolated from Black Sea water and M4.1 – from mussel liquor. The character of the revealed antimycotic activity was determined as fungicidal.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"13 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138956009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-02DOI: 10.18524/2307-4663.2023.1(57).274279
N. Hrytseva, L. Skivka
Aim. To investigate the prevalence of causative agents of ring rot and soft rot in the potato harvest by the DAS ELISA method. Materials and methods. Samples of the potato harvest from Ternopil, Zhytomyr, Kyiv, Cherkasy, Dnipropetrovsk, Donetsk, Mykolaiv, Odesa, and Kherson regions were used in the study. The detection of bacterial agents was conducted by enzyme-linked immunosorbent assay using commercial test systems LOEWE® Standard Complete Kit (Germany). Results. The highest percentage of affected potatoes was registered in the Donetsk region: 11.1% – by the Clavibacter seedonicus, 31.0% – by the Pectobacterium atrosepticum, and 8.9% – by both bacteria. The causative agent of soft rot was detected twice as often as a causative agent of ring rot in tuber samples from all regions, except the Ternopil region, where the predominance of Clavibacter sepedonicus was revealed. In addition to the Donetsk region, a high prevalence of the causative agent of soft rot was found in the potato harvest from Kyiv (10.2%) and Odesa (10.0%) regions, the highest abundance of the causative agent of ring rot was detected in the Ternopil region. The mixed bacterial infection of potato tubers was diagnosed in 6 areas, and the highest rate was detected in the Donetsk region. Conclusion. The analysis of the distribution of ring rot pathogen Clavibacter sepedonicus and soft rot pathogen Pectobacterium atrocepticum in the potato crop shows the presence of research pathogens in all studied regions. It was discovered the dominance of the representative of pectolytic bacteria in all regions except Ternopil, where the distribution of Clavibacter sepedonicus, the causative agent of ring rot, was dominated. High rates of spread of the studied pathogens were associated with increased local average annual precipitation, as well as with increased annual amplitude of this indicator.
{"title":"PREVALENCE OF CAUSATIVE AGENTS OF RING ROT CLAVIBACTER SEPEDONICUS, BLACKLEG AND WET ROT PECTOBACTERIUM ATROCEPTICUM IN THE 2021 YEAR POTATO HARVEST IN THE TERRITORY OF UKRAINE","authors":"N. Hrytseva, L. Skivka","doi":"10.18524/2307-4663.2023.1(57).274279","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.1(57).274279","url":null,"abstract":"Aim. To investigate the prevalence of causative agents of ring rot and soft rot in the potato harvest by the DAS ELISA method. Materials and methods. Samples of the potato harvest from Ternopil, Zhytomyr, Kyiv, Cherkasy, Dnipropetrovsk, Donetsk, Mykolaiv, Odesa, and Kherson regions were used in the study. The detection of bacterial agents was conducted by enzyme-linked immunosorbent assay using commercial test systems LOEWE® Standard Complete Kit (Germany). Results. The highest percentage of affected potatoes was registered in the Donetsk region: 11.1% – by the Clavibacter seedonicus, 31.0% – by the Pectobacterium atrosepticum, and 8.9% – by both bacteria. The causative agent of soft rot was detected twice as often as a causative agent of ring rot in tuber samples from all regions, except the Ternopil region, where the predominance of Clavibacter sepedonicus was revealed. In addition to the Donetsk region, a high prevalence of the causative agent of soft rot was found in the potato harvest from Kyiv (10.2%) and Odesa (10.0%) regions, the highest abundance of the causative agent of ring rot was detected in the Ternopil region. The mixed bacterial infection of potato tubers was diagnosed in 6 areas, and the highest rate was detected in the Donetsk region. Conclusion. The analysis of the distribution of ring rot pathogen Clavibacter sepedonicus and soft rot pathogen Pectobacterium atrocepticum in the potato crop shows the presence of research pathogens in all studied regions. It was discovered the dominance of the representative of pectolytic bacteria in all regions except Ternopil, where the distribution of Clavibacter sepedonicus, the causative agent of ring rot, was dominated. High rates of spread of the studied pathogens were associated with increased local average annual precipitation, as well as with increased annual amplitude of this indicator.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"160 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115969913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-02DOI: 10.18524/2307-4663.2023.1(57).276078
N. Tytarenko, N. Tesliuk, V. Ivanytsia
Microcloning is an effective method of plant reproduction that is actively developing in Ukraine for the mass propagation of valuable food crops such as blackberries. However, the problem of losing a large number of microclones often arises during the ex vitro acclimatization stage. Inoculation of the rhizosphere with potentially useful microorganisms could have a positive impact on the survival rate and biometric characteristics of acclimatized seedlings. The aim of this study was to establish the impact of 23 actinobacteria isolates on blackberry microclones (Rubus fruticosus L.) during acclimatization to ex vitro conditions, and to determine the protective potential and plant growth-promoting effects of these bacteria. Methods. The antagonistic properties of the experimental microorganisms were determined using the agar block method. Bacteria were inoculated into the rhizosphere of blackberry microclones before planting in the soil. Results. The antagonistic activity of actinobacteria against phytopathogenic fungi P. expansum, P. variotii, A. niger, C. cladosporioides, F. oxysporum, A. alternata, R. cerealis, and A. tenuissima was established. The positive effect of bacteria on micropropagated blackberry plants during acclimatization to ex vitro conditions resulted in an increase in the survival rate of microclones in the soil by 34.8%, average height of experimental plants by 2.0 cm, node number by 3.4 nodes, and leaf area by 0.4 cm2. Conclusion. It was established that isolates of mycelial actinobacteria Lim4, Myt7ch, Conc32, Conc4 were promising inoculants for ex vitro acclimatization of micropropagated plants and could be recommended for the subsequent research in order to establish the interaction mechanisms between these microorganisms and plants.
{"title":"IMPACT OF ACTINOBACTERIA ON THE GROWTH AND ACCLIMATIZATION OF MICROPROPAGATED RUBUS FRUTICOSUS L. PLANTS TO EX VITRO CONDITIONS","authors":"N. Tytarenko, N. Tesliuk, V. Ivanytsia","doi":"10.18524/2307-4663.2023.1(57).276078","DOIUrl":"https://doi.org/10.18524/2307-4663.2023.1(57).276078","url":null,"abstract":"Microcloning is an effective method of plant reproduction that is actively developing in Ukraine for the mass propagation of valuable food crops such as blackberries. However, the problem of losing a large number of microclones often arises during the ex vitro acclimatization stage. Inoculation of the rhizosphere with potentially useful microorganisms could have a positive impact on the survival rate and biometric characteristics of acclimatized seedlings. The aim of this study was to establish the impact of 23 actinobacteria isolates on blackberry microclones (Rubus fruticosus L.) during acclimatization to ex vitro conditions, and to determine the protective potential and plant growth-promoting effects of these bacteria. Methods. The antagonistic properties of the experimental microorganisms were determined using the agar block method. Bacteria were inoculated into the rhizosphere of blackberry microclones before planting in the soil. Results. The antagonistic activity of actinobacteria against phytopathogenic fungi P. expansum, P. variotii, A. niger, C. cladosporioides, F. oxysporum, A. alternata, R. cerealis, and A. tenuissima was established. The positive effect of bacteria on micropropagated blackberry plants during acclimatization to ex vitro conditions resulted in an increase in the survival rate of microclones in the soil by 34.8%, average height of experimental plants by 2.0 cm, node number by 3.4 nodes, and leaf area by 0.4 cm2. Conclusion. It was established that isolates of mycelial actinobacteria Lim4, Myt7ch, Conc32, Conc4 were promising inoculants for ex vitro acclimatization of micropropagated plants and could be recommended for the subsequent research in order to establish the interaction mechanisms between these microorganisms and plants.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"78 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126168932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-31DOI: 10.18524/2307-4663.2022.3(56).268585
I. V. Strashnova, K. S. Potapenko, N. Korotaeva, G. V. Lisyutin, I. P. Metelitsyna
SummaryThe rapid emergence of resistance by bacterial and fungal pathogens is a serious problem in the health care system, which causes the search for new promising producers of antimicrobial natural products in various ecological niches. Aim. To determine the antagonistic activity of streptomycetes isolated from the biological fouling of natural shell rock and mussels of the Odesa gulf of the Black Sea. Methods. The antagonistic activity of 19 and 14 strains of streptomycetes isolated from the fouling of shell rock and mussels of the Odesa gulf, respectively, were investigated. Streptomycetes were pre-cultivated on agar media Gause 1, Gause 2 and oat agar with sea salt (2%) at a temperature of 30 °C for 10 days. Antagonistic activity against 12 test cultures was determined by the block method. Results. All isolated marine streptomycetes are antagonists of at least one strain of the indicator microorganism. Antibiotic activity depended on the source of the streptomycetes isolation, culture medium and properties of specific strains of both producers and test cultures. The best activity of streptomycetes strains from shell rock was shown after cultivation on Gause 1 medium, and streptomycetes from mussels – after cultivation on Gause 2 medium. The zones of no growth of sensitive indicators ranged from 12,4±0,3 mm to 20,6±0,2 mm (under the influence of streptomycetes from shell rock) and from 12,4±0,2 mm to 39,7±0,2 mm (under the influence of streptomycetes from mussels). Streptomyces sp. Lim 2.2 (strain from a shell rock) inhibited the growth of 8 test cultures, and strains from mussels Streptomyces sp. Myt 4b and Myt 7ch – 10 test cultures. Indicator strains of gram-positive bacteria were the most sensitive to all streptomycetes, in particular, strain Staphylococcus aureus ATCC 25923 was most inhibited by metabolites of Streptomyces spp. Myt 12a and Myt 12b. Conclusions. Antagonistic activity of streptomycetes isolated from the Black Sea depended on the source of isolation, pre-cultivation medium and properties of both producer strains and indicator microorganisms. The greatest activity of streptomycete strains from shell rock and mussels was shown after preliminary cultivation, respectively, on Gause 1 and Gause 2 media against gram-positive bacteria. The best antibiotic potential was found in strains of Streptomyces sp. Lim 2.2, Lim 4, Lim 5.1 and Lim 7.2, isolated from the fouling of shell rock, and strains of Streptomyces sp. Myt 7b, Myt 7ch, Myt 12a and Myt 12b isolated from mussels.
{"title":"ANTAGONISTIC PROPERTIES OF THE BLACK SEA STREPTOMYCETES ISOLATED FROM THE FOULING OF SHELL ROCK AND MUSSELS","authors":"I. V. Strashnova, K. S. Potapenko, N. Korotaeva, G. V. Lisyutin, I. P. Metelitsyna","doi":"10.18524/2307-4663.2022.3(56).268585","DOIUrl":"https://doi.org/10.18524/2307-4663.2022.3(56).268585","url":null,"abstract":"SummaryThe rapid emergence of resistance by bacterial and fungal pathogens is a serious problem in the health care system, which causes the search for new promising producers of antimicrobial natural products in various ecological niches. Aim. To determine the antagonistic activity of streptomycetes isolated from the biological fouling of natural shell rock and mussels of the Odesa gulf of the Black Sea. Methods. The antagonistic activity of 19 and 14 strains of streptomycetes isolated from the fouling of shell rock and mussels of the Odesa gulf, respectively, were investigated. Streptomycetes were pre-cultivated on agar media Gause 1, Gause 2 and oat agar with sea salt (2%) at a temperature of 30 °C for 10 days. Antagonistic activity against 12 test cultures was determined by the block method. Results. All isolated marine streptomycetes are antagonists of at least one strain of the indicator microorganism. Antibiotic activity depended on the source of the streptomycetes isolation, culture medium and properties of specific strains of both producers and test cultures. The best activity of streptomycetes strains from shell rock was shown after cultivation on Gause 1 medium, and streptomycetes from mussels – after cultivation on Gause 2 medium. The zones of no growth of sensitive indicators ranged from 12,4±0,3 mm to 20,6±0,2 mm (under the influence of streptomycetes from shell rock) and from 12,4±0,2 mm to 39,7±0,2 mm (under the influence of streptomycetes from mussels). Streptomyces sp. Lim 2.2 (strain from a shell rock) inhibited the growth of 8 test cultures, and strains from mussels Streptomyces sp. Myt 4b and Myt 7ch – 10 test cultures. Indicator strains of gram-positive bacteria were the most sensitive to all streptomycetes, in particular, strain Staphylococcus aureus ATCC 25923 was most inhibited by metabolites of Streptomyces spp. Myt 12a and Myt 12b. Conclusions. Antagonistic activity of streptomycetes isolated from the Black Sea depended on the source of isolation, pre-cultivation medium and properties of both producer strains and indicator microorganisms. The greatest activity of streptomycete strains from shell rock and mussels was shown after preliminary cultivation, respectively, on Gause 1 and Gause 2 media against gram-positive bacteria. The best antibiotic potential was found in strains of Streptomyces sp. Lim 2.2, Lim 4, Lim 5.1 and Lim 7.2, isolated from the fouling of shell rock, and strains of Streptomyces sp. Myt 7b, Myt 7ch, Myt 12a and Myt 12b isolated from mussels.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"150 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115598997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-31DOI: 10.18524/2307-4663.2022.3(56).265806
N. Tesliuk, M. L. Lytvyn, T. V. Hudzenko
SummaryAim. To optimize the processes of microclonal propagation of Juglans regia in vitro by selecting the composition and consistency of the nutrient medium. Methods. The methods of in vitro culture establishment of initial explants and microclonal propagation were used. Parts of the stem and buds of young sprouts, germinated by the mini-greenhouse method from the seeds of Juglans regia, were used as initial explants. Murashige&Skoog (MS) and Driver&Kuniyuki (DKW) nutrient media were used to establish Juglans regia explants in vitro. Agar was used as a gelling agent. The solid, semi-liquid, and liquid media were prepared with addition of 7 g/l, 3.5 g/l and 0 g/l of agar, respectively. All experimental variants of the media were modified by adding the phytohormone of the cytokinin group 6-BAP. The pH of the medium was controlled at the level of 7.1–7.2. The established explants in vitro were cultivated at a temperature of + 25°C, a light intensity of 2500 lux, a relative humidity of 56–70% and a photoperiod 16/8 h light/dark. On the 3rd, 7th and 14th day, the survival rate, growth and development parameters of the initial explants were estimated. Results. It was found that the optimal nutrient medium for in vitro establishment and cultivation of Juglans regia is the DKW medium, on which the high survival rate of explants was observed (80%), as well as accelerated swelling of buds and their proliferation. It is proposed to use semi-liquid nutrient media for in vitro establishment of initial Juglans regia explants. The use of semi-liquid nutrient medium DKW for the initial stages of common walnut micropropagation in vitro contributed to the acceleration of the processes of reproduction of this culture by 2 days and improved the survival rate by 20% compared to solid medium. Conclusion. The use of semi-liquid nutrient medium DKW for introduction into the culture and cultivation of walnut (Juglans regia) in vitro is recommended to increase the survival rate of explants by 20%, as well as the accelerated activation of buds (2 days) and their further proliferation.
{"title":"OPTIMIZATION OF THE NUTRIENT MEDIUM FOR THE PRIMARY STAGES OF COMMON WALNUT MICROCLONAL PROPAGATION IN VITRO","authors":"N. Tesliuk, M. L. Lytvyn, T. V. Hudzenko","doi":"10.18524/2307-4663.2022.3(56).265806","DOIUrl":"https://doi.org/10.18524/2307-4663.2022.3(56).265806","url":null,"abstract":"SummaryAim. To optimize the processes of microclonal propagation of Juglans regia in vitro by selecting the composition and consistency of the nutrient medium. Methods. The methods of in vitro culture establishment of initial explants and microclonal propagation were used. Parts of the stem and buds of young sprouts, germinated by the mini-greenhouse method from the seeds of Juglans regia, were used as initial explants. Murashige&Skoog (MS) and Driver&Kuniyuki (DKW) nutrient media were used to establish Juglans regia explants in vitro. Agar was used as a gelling agent. The solid, semi-liquid, and liquid media were prepared with addition of 7 g/l, 3.5 g/l and 0 g/l of agar, respectively. All experimental variants of the media were modified by adding the phytohormone of the cytokinin group 6-BAP. The pH of the medium was controlled at the level of 7.1–7.2. The established explants in vitro were cultivated at a temperature of + 25°C, a light intensity of 2500 lux, a relative humidity of 56–70% and a photoperiod 16/8 h light/dark. On the 3rd, 7th and 14th day, the survival rate, growth and development parameters of the initial explants were estimated. Results. It was found that the optimal nutrient medium for in vitro establishment and cultivation of Juglans regia is the DKW medium, on which the high survival rate of explants was observed (80%), as well as accelerated swelling of buds and their proliferation. It is proposed to use semi-liquid nutrient media for in vitro establishment of initial Juglans regia explants. The use of semi-liquid nutrient medium DKW for the initial stages of common walnut micropropagation in vitro contributed to the acceleration of the processes of reproduction of this culture by 2 days and improved the survival rate by 20% compared to solid medium. Conclusion. The use of semi-liquid nutrient medium DKW for introduction into the culture and cultivation of walnut (Juglans regia) in vitro is recommended to increase the survival rate of explants by 20%, as well as the accelerated activation of buds (2 days) and their further proliferation.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122661963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-09-20DOI: 10.18524/2307-4663.2022.2(55).261778
M. D. Shtenikov, O. Zinchenko, V. V. Boldyreva
SummaryAim. To study the antagonistic activity of bacteria of genera Bacillus, Priestia and Paenibacillus at different conditions of cultivation. Methods. Antagonistically active spore-forming bacteria isolated from deep-sea bottom sediments of the Black Sea were used for the study. Determination of thermotypes was performed using the results of analysis of fatty acid profile parameters. Antagonistic activity to test strains of bacterial opportunistic pathogens was detected by the method of agar blocks on Gauze № 1 and Nutrient Agar media at different cultivation temperatures. Results. Aerobic bacilli of genera Bacillus, Priestia and Paenibacillus of all three thermotypes – thermotolerants, mesophiles and psychrotrophes, in general show lower antagonistic activity when cultured at 37 °C on both media, except for a marked increase in psychrotrophe antagonistic activity at 37 °С on Gauze № 1. Conclusions. It was established that belonging to a certain thermotype affects the character of the antagonistic activity of sporeforming bacteria. The antagonistic activity of mesophilic and thermotolerant bacteria at a higher temperature of cultivation is lower, and that of psychrotrophic bacteria at a higher temperature and on Gauze № 1 medium is higher.
{"title":"ANTAGONISTIC ACTIVITY OF MARINE BACTERIA OF BACILLUS, PRIESTIA AND PAENIBACILLUS GENERA OF DIFFERENT THERMOTYPES","authors":"M. D. Shtenikov, O. Zinchenko, V. V. Boldyreva","doi":"10.18524/2307-4663.2022.2(55).261778","DOIUrl":"https://doi.org/10.18524/2307-4663.2022.2(55).261778","url":null,"abstract":"SummaryAim. To study the antagonistic activity of bacteria of genera Bacillus, Priestia and Paenibacillus at different conditions of cultivation. Methods. Antagonistically active spore-forming bacteria isolated from deep-sea bottom sediments of the Black Sea were used for the study. Determination of thermotypes was performed using the results of analysis of fatty acid profile parameters. Antagonistic activity to test strains of bacterial opportunistic pathogens was detected by the method of agar blocks on Gauze № 1 and Nutrient Agar media at different cultivation temperatures. Results. Aerobic bacilli of genera Bacillus, Priestia and Paenibacillus of all three thermotypes – thermotolerants, mesophiles and psychrotrophes, in general show lower antagonistic activity when cultured at 37 °C on both media, except for a marked increase in psychrotrophe antagonistic activity at 37 °С on Gauze № 1. Conclusions. It was established that belonging to a certain thermotype affects the character of the antagonistic activity of sporeforming bacteria. The antagonistic activity of mesophilic and thermotolerant bacteria at a higher temperature of cultivation is lower, and that of psychrotrophic bacteria at a higher temperature and on Gauze № 1 medium is higher.","PeriodicalId":166598,"journal":{"name":"Microbiology&Biotechnology","volume":"485 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127568580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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