FeCo-Chitosan / DNA nanoparticles for gene transfer to MCF-7 breast cancer cells: preparation and characterization

A. Abdolmaleki, Z. O. Khudhur, S. W. Smail, A. Asadi, Amin Amani
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Abstract

Background: Researchers have seen gene therapy as one of the most important techniques for treating illnesses including cancer and a range of genetic problems in recent years. The capacity of FeCo-Chitosan nanoparticles for gene transport into MCF-7 cells was explored in this study Methods: FeCo-Chitosan/DNA nanoparticles were prepared. Then, physicochemical features of nanoparticles, were assessed using SEM. Also, biological features of the nanoparticles including biocompatibility, DNA protection, DNA release, and gene transfer capacity to MCF-7 cells were studied,. Results: Results showed that FeCo-Chitosan / DNA nanoparticles exhibited a spherical shape with an average size of around 200 nm. The zeta potential of the FeCo-Chitosan/DNA complex increased with increasing the concentration of FeCo-Chitosan nanoparticles in the FeCo-Chitosan/DNA complex. Electrophoretic analyses showed that FeCo-Chitosan/DNA nanoparticles protects DNA against nuclease degradation and ultrasonic damage. Also, MTT test revealed that FeCo-Chitosan nanoparticles had a good biocompatibility. Conclusions: FeCo-Chitosan nanoparticles may safely transfer and release DNA to MCF-7 cells, according to fluorescence microscopy and flow cytometry studies. These findings also revealed that increasing the concentration of FeCo-Chitosan in the FeCo-Chitosan/DNA complex improved gene transfer efficiency
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用于 MCF-7 乳腺癌细胞基因转移的铁钴壳聚糖/DNA 纳米粒子:制备与表征
背景:近年来,研究人员已将基因疗法视为治疗包括癌症和一系列遗传问题在内的疾病的最重要技术之一。本研究探讨了铁钴壳聚糖纳米颗粒向 MCF-7 细胞转运基因的能力:制备铁钴壳聚糖/DNA 纳米颗粒。然后,使用扫描电镜评估纳米颗粒的物理化学特征。此外,还研究了纳米颗粒的生物特性,包括生物相容性、DNA 保护、DNA 释放以及向 MCF-7 细胞转移基因的能力。结果显示结果表明,FeCo-壳聚糖/DNA 纳米粒子呈球形,平均尺寸约为 200 nm。随着 FeCo-Citosan/DNA 复合物中 FeCo-Citosan 纳米粒子浓度的增加,FeCo-壳聚糖/DNA 复合物的 zeta 电位也随之增加。电泳分析表明,FeCo-壳聚糖/DNA 纳米粒子能保护 DNA 免受核酸酶降解和超声波破坏。此外,MTT 测试表明,FeCo-壳聚糖纳米粒子具有良好的生物相容性。结论荧光显微镜和流式细胞术研究表明,钴壳聚糖铁纳米粒子可安全地转移和释放 DNA 到 MCF-7 细胞中。这些研究结果还表明,提高铁钴壳聚糖/DNA 复合物中铁钴壳聚糖的浓度可提高基因转移效率。
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