Variations of activity and community structure of nitrite-driven anaerobic methanotrophs in soils between native and invasive species in China's coastal wetlands

Abstract

Nitrite-driven anaerobic oxidation of methane (nitrite-driven AOM), mediated by ‘Candidatus Methylomirabilis oxyfera’ (M. oxyfera)-related bacteria, is a newly-discovered CH₄ consumption process in coastal wetlands. Although Spartina alterniflora invasion significantly affects CH₄ emissions from coastal wetlands, its impact on the nitrite-driven AOM process and the underlying mechanisms remain unknown. Here, we examined nitrite-driven AOM activity and M. oxyfera-related bacterial community in four coastal wetlands along the southeastern coast of China, under invasive Spartina alterniflora and native plants, including Kandelia candel, Avicennia marina or Phragmites australis. Linear mixed-effects models indicated that the Spartina alterniflora invasion stimulated the overall nitrite-driven AOM activity by an average of 61.5% in coastal wetlands (p < 0.05), but had no impact on the M. oxyfera-related bacterial abundance (p > 0.05). The nitrite-driven AOM activity was 7.1 times higher under Spartina alterniflora than under native species in Yueqing Bay (p < 0.05), and was 34.7%, 8.9% and 15.1% higher under Spartina alterniflora than under native species in Hengsha Island, Jiulong River and Zhanjiang, respectively (p > 0.05). Spartina alterniflora invasion increased the bacterial abundance in Yueqing Bay and Jiulong River Estuary by 6.8 and 7.6 times, respectively, while decreased the abundance by 34.4% and 51.4%, respectively, in Hengsha Island and Zhanjiang (p > 0.05). The partial least squares path model indicated an indirect impact of Spartina alterniflora invasion on the nitrite-driven AOM activity through its effect on soil properties, primarily including dissolved organic carbon content and nitrate content. The Spartina alterniflora invasion did not greatly alter M. oxyfera-related bacterial community. Overall, we shed new light on the potential impact of Spartina alterniflora invasion on CH₄ cycling in coastal wetlands.