Aerobic interval training preconditioning protocols inhibit isoproterenol-induced pathological cardiac remodeling in rats: Implications on oxidative balance, autophagy, and apoptosis

IF 2.3 Q2 SPORT SCIENCES Sports Medicine and Health Science Pub Date : 2024-02-02 DOI:10.1016/j.smhs.2024.01.010
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Abstract

This study aimed to investigate the potential cardioprotective effects of moderate and high-intensity aerobic interval training (MIIT and HIIT) preconditioning. The focus was on histological changes, pro-oxidant-antioxidant balance, autophagy initiation, and apoptosis in myocardial tissue incited by isoproterenol-induced pathological cardiac remodeling (ISO-induced PCR). Male Wistar rats were randomly divided into control (n ​= ​6), ISO (n ​= ​8), MIIT (n ​= ​4), HIIT (n ​= ​4), MIIT ​+ ​ISO (n ​= ​8), and HIIT ​+ ​ISO (n ​= ​8) groups. The MIIT and HIIT protocols were administered for 10 weeks, followed by the induction of cardiac remodeling using subcutaneous injection of ISO (100 ​mg/kg for two consecutive days). Alterations in heart rate (HR), mean arterial pressure (MAP), rate pressure product (RPP), myocardial oxygen consumption (MV˙O2), cardiac hypertrophy, histopathological changes, pro-oxidant-antioxidant balance, autophagy biomarkers (Beclin-1, Atg7, p62, LC3 I/II), and apoptotic cell distribution were measured. The findings revealed that the MIIT ​+ ​ISO and HIIT ​+ ​ISO groups demonstrated diminished myocardial damage, hemorrhage, immune cell infiltration, edema, necrosis, and apoptosis compared to ISO-induced rats. MIIT and HIIT preconditioning mitigated HR, enhanced MAP, and preserved MV˙O2 and RPP. The pro-oxidant-antioxidant balance was sustained in both MIIT ​+ ​ISO and HIIT ​+ ​ISO groups, with MIIT primarily inhibiting pro-apoptotic autophagy progression through maintaining pro-oxidant-antioxidant balance, and HIIT promoting pro-survival autophagy. The results demonstrated the beneficial effects of both MIIT and HIIT as AITs preconditioning in ameliorating ISO-induced PCR by improving exercise capacity, hemodynamic parameters, and histopathological changes. Some of these protective effects can be attributed to the modulation of cardiac apoptosis, autophagy, and oxidative stress.

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有氧间歇训练预处理方案可抑制异丙肾上腺素诱导的大鼠病理性心脏重塑:对氧化平衡、自噬和细胞凋亡的影响
本研究旨在探讨中度和高强度有氧间歇训练(MIIT 和 HIIT)预处理对心脏的潜在保护作用。研究重点是异丙托品醇诱导的病理性心脏重塑(ISO诱导的PCR)所引发的心肌组织的组织学变化、促氧化-抗氧化平衡、自噬启动和细胞凋亡。雄性 Wistar 大鼠被随机分为对照组(n = 6)、ISO 组(n = 8)、MIIT 组(n = 4)、HIIT 组(n = 4)、MIIT + ISO 组(n = 8)和 HIIT + ISO 组(n = 8)。MIIT和HIIT方案实施10周,然后通过皮下注射ISO(100毫克/千克,连续两天)诱导心脏重塑。研究人员测量了心率(HR)、平均动脉压(MAP)、速率压力积(RPP)、心肌耗氧量(MV˙O2)、心脏肥大、组织病理学变化、促氧化剂-抗氧化剂平衡、自噬生物标志物(Beclin-1、Atg7、p62、LC3 I/II)和凋亡细胞分布的变化。研究结果显示,与 ISO 诱导的大鼠相比,MIIT + ISO 组和 HIIT + ISO 组的心肌损伤、出血、免疫细胞浸润、水肿、坏死和细胞凋亡均有所减轻。MIIT 和 HIIT 预处理减轻了 HR,提高了 MAP,保护了 MV˙O2 和 RPP。MIIT + ISO组和HIIT + ISO组都维持了促氧化-抗氧化平衡,其中MIIT主要是通过维持促氧化-抗氧化平衡来抑制促凋亡的自噬过程,而HIIT则促进了促生存的自噬过程。研究结果表明,MIIT 和 HIIT 作为 AITs 预处理,通过改善运动能力、血液动力学参数和组织病理学变化,对改善 ISO 诱导的 PCR 有益。其中一些保护作用可归因于对心脏凋亡、自噬和氧化应激的调节。
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来源期刊
Sports Medicine and Health Science
Sports Medicine and Health Science Health Professions-Physical Therapy, Sports Therapy and Rehabilitation
CiteScore
5.50
自引率
0.00%
发文量
36
审稿时长
55 days
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