Impact of two Erwinia sp. on the response of diverse Pisum sativum genotypes under salt stress

IF 3.4 3区 生物学 Q1 PLANT SCIENCES Physiology and Molecular Biology of Plants Pub Date : 2024-02-25 DOI:10.1007/s12298-024-01419-8
Houda Ilahi, Elisa Zampieri, Cristiana Sbrana, Francesca Brescia, Luca Giovannini, Roghayyeh Mahmoudi, Gholamreza Gohari, Mustapha Missbah El Idrissi, Mohamed Najib Alfeddy, Martino Schillaci, Lahcen Ouahmane, Alice Calvo, Fabiano Sillo, Vasileios Fotopoulos, Raffaella Balestrini, Bacem Mnasri
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Abstract

Currently, salinization is impacting more than 50% of arable land, posing a significant challenge to agriculture globally. Salt causes osmotic and ionic stress, determining cell dehydration, ion homeostasis, and metabolic process alteration, thus negatively influencing plant development. A promising sustainable approach to improve plant tolerance to salinity is the use of plant growth-promoting bacteria (PGPB). This work aimed to characterize two bacterial strains, that have been isolated from pea root nodules, initially called PG1 and PG2, and assess their impact on growth, physiological, biochemical, and molecular parameters in three pea genotypes (Merveille de Kelvedon, Lincoln, Meraviglia d’Italia) under salinity. Bacterial strains were molecularly identified, and characterized by in vitro assays to evaluate the plant growth promoting abilities. Both strains were identified as Erwinia sp., demonstrating in vitro biosynthesis of IAA, ACC deaminase activity, as well as the capacity to grow in presence of NaCl and PEG. Considering the inoculation of plants, pea biometric parameters were unaffected by the presence of the bacteria, independently by the considered genotype. Conversely, the three pea genotypes differed in the regulation of antioxidant genes coding for catalase (PsCAT) and superoxide dismutase (PsSOD). The highest proline levels (212.88 μmol g−1) were detected in salt-stressed Lincoln plants inoculated with PG1, along with the up-regulation of PsSOD and PsCAT. Conversely, PG2 inoculation resulted in the lowest proline levels that were observed in Lincoln and Meraviglia d’Italia (35.39 and 23.67 μmol g−1, respectively). Overall, this study highlights the potential of these two strains as beneficial plant growth-promoting bacteria in saline environments, showing that their inoculation modulates responses in pea plants, affecting antioxidant gene expression and proline accumulation.

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两种 Erwinia sp. 对盐胁迫下不同 Pisum sativum 基因型反应的影响
目前,50% 以上的耕地受到盐碱化的影响,这给全球农业带来了巨大挑战。盐分会造成渗透和离子压力,决定细胞脱水、离子平衡和新陈代谢过程的改变,从而对植物生长产生负面影响。利用植物生长促进细菌(PGPB)来提高植物对盐分的耐受性是一种很有前景的可持续方法。这项研究旨在描述从豌豆根瘤中分离出来的两种细菌菌株(最初称为 PG1 和 PG2)的特征,并评估它们对三种豌豆基因型(Merveille de Kelvedon、Lincoln 和 Meraviglia d'Italia)在盐度条件下的生长、生理、生化和分子参数的影响。对细菌菌株进行了分子鉴定,并通过体外试验评估其促进植物生长的能力。两株菌株均被鉴定为 Erwinia sp.,在体外显示出 IAA 的生物合成、ACC 脱氨酶活性以及在 NaCl 和 PEG 存在下的生长能力。考虑到植物的接种问题,豌豆的生物计量参数不受细菌存在的影响,与所考虑的基因型无关。相反,三种豌豆基因型在编码过氧化氢酶(PsCAT)和超氧化物歧化酶(PsSOD)的抗氧化基因的调控方面存在差异。接种 PG1 的盐胁迫林肯植株脯氨酸含量最高(212.88 μmol g-1),同时 PsSOD 和 PsCAT 也上调。相反,接种 PG2 会导致林肯和 Meraviglia d'Italia 的脯氨酸水平最低(分别为 35.39 和 23.67 μmol g-1)。总之,本研究强调了这两种菌株在盐碱环境中作为有益植物生长促进菌的潜力,表明它们的接种会调节豌豆植物的反应,影响抗氧化基因的表达和脯氨酸的积累。
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来源期刊
CiteScore
7.10
自引率
0.00%
发文量
126
期刊介绍: Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.
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