Effectiveness of Pseudomonas Aeruginosa in Destruction of Harmful Lipids in Lipidemia Patients

Abstract

Pseudomonas aeruginosa is a type of harmful microorganism that belongs to a large group of Gram-negative bacteria, these bacteria can secrete many extracellular enzymes such as microbial lipase and oxidase that act on certain lipids.  This study aims to activate certain enzymes of P. aeruginosa like lipase and oxidase in order to decrease high plasma triglycerides and cholesterol in lipidemia patients. Serum can also be used due to the fact that lipids are found in both serum and plasma. Five ml. of blood were collected from 100 lipidemia patients at Baghdad Teaching Hospital laboratories in Medical city, P. aeruginosa was isolated from medical wastes; cultured on bacterial media, gram stained and biochemically tested in vitro. Methods depend on inoculation of bacterial broth containing colonies of P. aeruginosa adjusted to McFarland standard turbidity with 50% of blood plasma were added, then cultures were incubated at 37°C for 6 days, activation of bacterial lipase and oxidase were done during the incubation period by addition of certain activators to the broth like glycogen liquid. The results showed that 100% of patients had cholesterol <200 mg/dl and 99% of patients had normal triglyceride level (<200mg/dl) after bacterial inoculation and activation of microbial lipase and oxidase. As conclusion, the high plasma lipids level of patients was decreased four times by P. aeruginosa may be due to the activation of microbial lipase and oxidase which could needs to future research to extract them and used them as treatment for these cases.