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The Relationship between Vertical Kinematic Eddy Heat Flux, Air Temperature and Turbulent Kinetic Energy in Atmospheric Boundary Layer: Baghdad City 大气边界层中垂直运动涡旋热通量、空气温度和湍流动能之间的关系:巴格达市
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1420
Nagham Abbas Mohammed
Studying the heat flux in the boundary layer and understanding its relationship to the various atmospheric variables reflects positively on understanding the nature of turbulence in this layer and thus understanding the nature of the spread and movement of pollutants and the transmission and distribution of energy in this layer, also, the vertical heat flux is considered a significant influence on the movement of buoyancy and stability in the boundary layer and its effect on the horizontal wind movement, and therefore it is considered one of the important studies indirectly involved in the estimates of wind energy production, pollutant diffusion, and turbulence. This study involved the calculation of the Eddy Heat Flux and turbulent kinetic energy across Baghdad city. Our investigation revealed a correlation between the Eddy Heat Flux, temperature, and Turbulent Kinetic Energy (TKE). The observations have been made for wind speed with three components (u, v, w) and temperature by using a fast-response anemometer. As for the atmospheric pressure data, it was obtained from the automatic weather station located in the department of Atmospheric Sciences at Mustansiriyah University. The range of observations extended through the duration of thirty days for 24 hours from 1st July 2016 to 30th July 2016 every second. The maximum Eddy Heat Flux value was 0.092 J/(m2.s) at 10:00 on 23th July 2016, while the minimum Eddy Heat Flux value was -0.013 J/(m2.s) at 21:00 hour on 25th July 2016, the negative sign refer to a change in the direction of heat transfer,. It was also found that there is a positive relationship between the eddy heat flux and temperatures with a correlation coefficient of 0.93, as well as between the eddy heat flux and the turbulent kinetic energy with a correlation coefficient of 0.8.
研究边界层中的热通量并了解其与各种大气变量之间的关系,对了解该层中湍流的性质,从而了解污染物在该层中的扩散和运动性质以及能量的传输和分布具有积极意义,此外,垂直热通量被认为对边界层中浮力和稳定性的运动及其对水平风运动的影响具有重要影响,因此被认为是间接涉及风能生产、污染物扩散和湍流估计的重要研究之一。本研究涉及巴格达市内涡热通量和湍流动能的计算。我们的调查显示,涡热通量、温度和湍流动能(TKE)之间存在相关性。我们使用快速反应风速计对风速的三个分量(u、v、w)和温度进行了观测。至于大气压力数据,则是从穆斯坦西利亚大学大气科学系的自动气象站获得的。观测范围从 2016 年 7 月 1 日至 2016 年 7 月 30 日,持续 30 天,每秒 24 小时。2016 年 7 月 23 日 10:00 时涡旋热通量最大值为 0.092 J/(m2.s),而 2016 年 7 月 25 日 21:00 时涡旋热通量最小值为-0.013 J/(m2.s),负号表示热量传递方向发生变化。研究还发现,涡热通量与温度之间存在正相关关系,相关系数为 0.93;涡热通量与湍流动能之间也存在正相关关系,相关系数为 0.8。
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引用次数: 0
Effectiveness of Pseudomonas Aeruginosa in Destruction of Harmful Lipids in Lipidemia Patients 绿脓杆菌对血脂病患者有害脂质的有效破坏作用
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1427
Suhaib Khalid Ibrahim
Pseudomonas aeruginosa is a type of harmful microorganism that belongs to a large group of Gram-negative bacteria, these bacteria can secrete many extracellular enzymes such as microbial lipase and oxidase that act on certain lipids.  This study aims to activate certain enzymes of P. aeruginosa like lipase and oxidase in order to decrease high plasma triglycerides and cholesterol in lipidemia patients. Serum can also be used due to the fact that lipids are found in both serum and plasma. Five ml. of blood were collected from 100 lipidemia patients at Baghdad Teaching Hospital laboratories in Medical city, P. aeruginosa was isolated from medical wastes; cultured on bacterial media, gram stained and biochemically tested in vitro. Methods depend on inoculation of bacterial broth containing colonies of P. aeruginosa adjusted to McFarland standard turbidity with 50% of blood plasma were added, then cultures were incubated at 37°C for 6 days, activation of bacterial lipase and oxidase were done during the incubation period by addition of certain activators to the broth like glycogen liquid. The results showed that 100% of patients had cholesterol <200 mg/dl and 99% of patients had normal triglyceride level (<200mg/dl) after bacterial inoculation and activation of microbial lipase and oxidase. As conclusion, the high plasma lipids level of patients was decreased four times by P. aeruginosa may be due to the activation of microbial lipase and oxidase which could needs to future research to extract them and used them as treatment for these cases. 
铜绿假单胞菌是一种有害微生物,属于革兰氏阴性菌中的一大类,这些细菌能分泌多种胞外酶,如微生物脂肪酶和氧化酶,可作用于某些脂类。 本研究旨在激活铜绿假单胞菌的某些酶,如脂肪酶和氧化酶,以降低血脂高患者的血浆甘油三酯和胆固醇。由于血脂同时存在于血清和血浆中,因此也可使用血清。从医疗废物中分离出铜绿假单胞菌,在细菌培养基上进行培养、革兰氏染色和体外生化测试。方法是将含有铜绿假单胞菌菌落的细菌培养基接种到调节到麦克法兰标准浊度的菌液中,加入 50%的血浆,然后将培养物在 37°C 下培养 6 天,在培养期间通过在菌液中加入某些活化剂(如糖原液)来激活细菌的脂肪酶和氧化酶。结果显示,在接种细菌并激活微生物脂肪酶和氧化酶后,100% 的患者胆固醇水平<200 毫克/分升,99% 的患者甘油三酯水平正常(<200 毫克/分升)。总之,铜绿假单胞菌使患者的高血脂水平降低了四倍,这可能是由于铜绿假单胞菌激活了微生物脂肪酶和氧化酶。
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引用次数: 0
Image Analysis and Detection of Olive Leaf Diseases Using Recurrent Neural Networks 利用递归神经网络对橄榄叶片病害进行图像分析和检测
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1416
Mohsin R. Kareem
The widespread adoption of DL has led to a rise in academic interest in image recognition approaches, enabling applications such as automated image classification and the detection of plant diseases. The world's largest producer of olives is Morocco. Plant health might be harmed by illnesses, which therefore affects its development. Numerous illnesses affecting olive leaves specifically target crop growth rate. The objective of this research is to create deep RNNs to identify olive plant illnesses using a collection of leaf images, collected from various sources (Disease note The peacock eye falls on olive trees, Field Guide to Olive Pests, Diseases and Disorders in Australia. Thus, this technique is the best RNN model and is employed in further applications to enhance diagnostic measurements regarding olive leaves and other plant leaves.
DL 的广泛应用提高了学术界对图像识别方法的兴趣,使自动图像分类和植物病害检测等应用成为可能。摩洛哥是世界上最大的橄榄生产国。植物健康可能会受到疾病的危害,从而影响其生长发育。影响橄榄叶片的许多疾病都特别针对作物的生长速度。这项研究的目的是创建深度 RNN,利用从各种渠道收集到的叶片图像(病害注释:孔雀眼落在橄榄树上、澳大利亚橄榄害虫、疾病和紊乱现场指南)来识别橄榄树的病害。因此,该技术是最佳的 RNN 模型,可用于进一步的应用中,以增强对橄榄叶和其他植物叶片的诊断测量。
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引用次数: 0
Optical and Degradation Characteristics of Green Synthesized Cornstarch-Base Bioorganic Polymer 绿色合成玉米淀粉基生物有机聚合物的光学特性和降解特性
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1404
Marowa Yass, Ahmed Al-Haddad, Ali Jaafar Sadeq
Chemical-base polymers are mostly related to environmental pollution since their poisonous, non-biodegradable wastes, and negatively impact plants, animals, and people. In many locations where petroleum-based plastics are used, bioorganic polymers and biomaterials can play a promising role and are accepted. In one such attempt, glycerol was used as a natural plasticizer, and cornstarch was used to create bioorganic polymers and biocomposites. Resulting in a highly degradable bioorganic polymer in water and soil, display FE-SEM images of a BOP's top and cross-section views, flat surface and smooth, the inner structure of BOP does not demonstrate any bubbles, cracking, flipping, or splitting., estimate the optical band gap indirect of BOP of 3.69 eV that can absorb 96% of UV light and transmit 98 % of visible light. The FTIR assay detects the functional aggregates of the BOP as well as the presence of a hydrogen bond in the BOP matrix. Raman spectroscopy detection composition and scope distribution of the various components in a prepared BOP. The decay constants of BOP were gaged as 12.4 ± 1.8 and 3.49 ± 0.41 for the water and soil, respectively. The decay values show an acceptable variation due to the nature of the BOP on one side and the water and soil on the other side. These findings conclude diverse applications highlighting BOP as a candidate, e.g., UV screening or protective layer, food packaging, and replacing the chemical fertilizer with BOP agent to deliver the organic substances to the plants.
化学基聚合物大多与环境污染有关,因为它们是有毒的、不可生物降解的废物,并对植物、动物和人类产生负面影响。在许多使用石油基塑料的地方,生物有机聚合物和生物材料可以发挥很好的作用,并被人们所接受。在一次尝试中,甘油被用作天然增塑剂,玉米淀粉被用来制造生物有机聚合物和生物复合材料。BOP 的顶视图和横截面图的 FE-SEM 图像显示,BOP 表面平整光滑,内部结构没有任何气泡、裂纹、翻转或分裂,估计 BOP 的光带隙间接为 3.69 eV,可吸收 96% 的紫外光,透过 98% 的可见光。傅立叶变换红外光谱检测 BOP 的功能聚集体以及 BOP 基质中是否存在氢键。拉曼光谱检测制备的 BOP 中各种成分的组成和范围分布。水和土壤中 BOP 的衰变常数分别为 12.4 ± 1.8 和 3.49 ± 0.41。由于一边是 BOP,另一边是水和土壤,衰变值显示了可接受的变化。这些研究结果总结了 BOP 的多种应用,如紫外线屏蔽或保护层、食品包装,以及用 BOP 取代化肥向植物输送有机物质等。
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引用次数: 0
Evaluation of Lipid Profile and Oxidative Stress of Workers in Fast Food Restaurants and The Risk of Atherosclerosis 快餐店员工血脂谱和氧化应激与动脉粥样硬化风险评估
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1405
Muntaha A. Mohammed, Falah S. Al-Fartusie, Mohammed Z. Thani
Cooking fumes and biomass smoke pose significant threats to air degradation and human health, especially to people who work in this field on a daily basis, due to the large amounts of hazardous pollutants released from grilling activities. Chronic exposure to these fumes is associated with a risk of inflammation and oxidative stress, which can increase the risk of atherosclerosis. In this study, the lipid profile Glutathion (GSH) and Malondialdehyde (MDA) of workers in fast food restaurants and patients with atherosclerosis were evaluated. The study included 40 Atherosclerosis patients, 40 fast food workers, and 40 healthy individuals as a control group, the ages ranges of (20-40) years. The obtained results indicated a significant increase in cholesterol, triglycerides, LDL, and VLDL and a decrease in HDL (p < 0.01) in patients and fast-food workers compared to control group. The results also indicated a significant increase in the level of MDA, and a significant decrease in the level of GSH for patients and fast-food workers compared to the control. In conclusion, it appears that fast food workers experience oxidative stress and disruption in lipid metabolism that may increase the risk of atherosclerosis. Therefore, safety and quality control measures must be strengthened to reduce the occupational risks of fast-food restaurant workers.    
由于烧烤活动释放出大量有害污染物,烹饪油烟和生物质烟雾对空气质量下降和人类健康构成重大威胁,尤其是对每天在这一领域工作的人而言。长期暴露于这些烟雾中会导致炎症和氧化应激风险,从而增加动脉粥样硬化的风险。在这项研究中,对快餐店工人和动脉粥样硬化患者的血脂谱谷胱甘肽(GSH)和丙二醛(MDA)进行了评估。研究对象包括 40 名动脉粥样硬化患者、40 名快餐店员工和 40 名健康人(年龄在 20-40 岁之间)作为对照组。结果表明,与对照组相比,患者和快餐店员工的胆固醇、甘油三酯、低密度脂蛋白和超低密度脂蛋白明显增加,高密度脂蛋白减少(p < 0.01)。结果还表明,与对照组相比,患者和快餐店员工的 MDA 水平明显升高,GSH 水平明显降低。总之,快餐店员工似乎经历了氧化应激和脂质代谢紊乱,这可能会增加动脉粥样硬化的风险。因此,必须加强安全和质量控制措施,以降低快餐店员工的职业风险。
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引用次数: 0
Study of Sky Radio Frequency Interference Background Level Behavior During the Partial Solar Eclips on 25 Oct. 2022 at The Baghdad University Location 2022 年 10 月 25 日巴格达大学日偏食期间天空无线电频率干扰背景水平行为研究
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1460
Zina Fadel Kahdim, Kamal M. Abood
Radiofrequency interference (RFI) describes the weakening of radio astronomy transmissions caused by man-made radio signal noise in radio telescope observation indicates the level of radio frequency noise in the sky at all times, regardless of any astronomical sources. This noise is affected by various sources, including Earth's atmosphere or solar activity and all communications equipment operating near the radio telescope. In this study, the background noise which includes RFI was measured using HSA2000 series receiver spectrum analyzer, which connected with two dipole antennas in different frequency bands (15-80 MHz) low band antenna, and (110-240 MHz) high band antenna during the partial solar eclipse in Baghdad on October 25, 2022, at (12:00-15:00), as a simple radio telescope to observe the radio astronomical background level in the sky. It is clear that the average amount of RFI level during a partial solar eclipse (-83.249 dBm for LBA decreased by about (0.93) from the value on a normal day (-77.495 dBm). And For HBA -84.711 dBm decreased by about (0.85) from the value on a normal day (-72.784 dBm). Also, it was noticed that these amounts are approximately equal to the RFI level at night (-82.352 dBm for LBA and -82.739 dBm for HBA). It is (0.98) for LBA and (0.97) for HBA, respectively. This research confirms that despite the short duration of the partial eclipse, the average value during the partial eclipse period is close to the average values during the night period due to the decrease in the electronic density of the ionosphere, especially at low frequencies, and the FRI of ground disturbances as a result of the activities of human from the surrounding the monitoring area.  also, found that at the monitoring point, there is a good relationship between the time of the maximum partial eclipse and the peaks of signal gain strength.
射频干扰(RFI)描述的是射电望远镜观测中人为射电信号噪声对射电天文传输造成的削弱,它表示天空中任何时候的射频噪声水平,与任何天文来源无关。这种噪声受到各种来源的影响,包括地球大气层或太阳活动以及在射电望远镜附近运行的所有通信设备。在本研究中,使用 HSA2000 系列接收器频谱分析仪测量了包括射频干扰在内的背景噪声,该接收器连接了两个不同频段的偶极子天线(15-80 MHz)低频段天线和(110-240 MHz)高频段天线,在 2022 年 10 月 25 日巴格达日偏食期间(12:00-15:00),作为一个简单的射电望远镜来观测天空中的射电天文背景水平。很明显,日偏食期间的平均射频干扰电平(LBA 为 -83.249 dBm)比平日的值(-77.495 dBm)降低了约 (0.93)。而高分辨率辐射的 -84.711 dBm 则比平日的值 (-72.784 dBm) 降低了约 (0.85)。此外,我们还注意到,这些数值与夜间的射频干扰电平(LBA 为 -82.352 dBm,HBA 为 -82.739 dBm)大致相等。LBA 和 HBA 分别为 (0.98) 和 (0.97)。这项研究证实,尽管日偏食持续时间较短,但日偏食期间的平均值与夜间的平均值相近,原因是电离层电子密度(尤其是低频电子密度)降低,以及监测区域周围人类活动造成的地面干扰 FRI。
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引用次数: 0
Enhancing Meditation Techniques and Insights Using Feature Analysis of Electroencephalography (EEG) 利用脑电图(EEG)特征分析提高冥想技巧和洞察力
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1457
Zahraa Maki Khadam, A. Abdulhameed, Ahmed Hammad
Through a Bluetooth connection between the Muse 2 device and the meditation app, leveraging IoT capabilities. The methodology encompasses data collection, preprocessing, feature extraction, and model training, all while utilizing Internet of Things (IoT) functionalities. The Muse 2 device records EEG data from multiple electrodes, which is then processed and analyzed within a mobile meditation platform. Preprocessing steps involve eliminating redundant columns, handling missing data, normalizing, and filtering, making use of IoT-enabled techniques. Feature extraction is carried out on EEG signals, utilizing statistical measures such as mean, standard deviation, and entropy. Three different models, including Support Vector Machine (SVM), Random Forest, and Multi-Layer Perceptron (MLP), are trained using the preprocessed data, incorporating Internet of Things (IoT) based methodologies. Model performance is assessed using metrics like accuracy, precision, recall, and F1-score, highlighting the effectiveness of IoT-driven techniques. Notably, the MLP and Random Forest models demonstrate remarkable accuracy and precision, underlining the potential of this IoT-integrated approach. Specifically, the three models achieved high accuracies, with Random Forest leading at 0.999, followed by SVM at 0.959 and MLP at 0.99. This study not only contributes to the field of brain-computer interfaces and assistive technologies but also showcases a viable method to seamlessly integrate the Muse 2 device into meditation practices, promoting self-awareness and mindfulness with the added power of IoT technology.
通过 Muse 2 设备与冥想应用程序之间的蓝牙连接,充分利用物联网功能。该方法包括数据收集、预处理、特征提取和模型训练,同时利用了物联网(IoT)功能。Muse 2 设备从多个电极记录脑电图数据,然后在移动冥想平台上进行处理和分析。预处理步骤包括利用物联网技术消除冗余列、处理缺失数据、归一化和过滤。利用平均值、标准偏差和熵等统计量对脑电信号进行特征提取。利用预处理数据,结合基于物联网(IoT)的方法,训练了三种不同的模型,包括支持向量机(SVM)、随机森林(Random Forest)和多层感知器(MLP)。使用准确率、精确度、召回率和 F1 分数等指标对模型性能进行了评估,突出了物联网驱动技术的有效性。值得注意的是,MLP 和随机森林模型表现出了出色的准确度和精确度,凸显了这种物联网集成方法的潜力。具体来说,这三种模型都达到了很高的精确度,其中随机森林模型以 0.999 的精确度遥遥领先,其次是 SVM 的 0.959 和 MLP 的 0.99。这项研究不仅为脑机接口和辅助技术领域做出了贡献,还展示了将 Muse 2 设备无缝集成到冥想实践中的可行方法,通过物联网技术的附加功能促进自我意识和正念。
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引用次数: 0
Performance and Efficiency of an Autoclave Made from Local Materials in Winter and Summer in Iraq 用当地材料制造的高压灭菌器在伊拉克冬季和夏季的性能和效率
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1452
Nada S. Saleh, Hazim H. Hussain, Ahmed Abdelhalim
A solar-powered autoclave, made of a parabolic dish reflector that collects solar radiation at the focal point to heat a vessel, was established and tested. Thermal performance was tested and compared between months June and December 2022 through thermal energy efficiency equations and the total required thermal energy to evaporate water and the required thermal energy, where the highest values in June 2022 were 9%, 46 W, and 18 W, respectively at 9:00 am. Because solar radiation values are low, the energy required for heating is high and the lowest values were 0.2%, 1.9 W, and 0.5 W, respectively at 12:30 pm Because solar radiation values are high, the energy required for heating is less. While the highest values on December 2022 were 22%, 23 W, and 14 W, respectively, at 9:00 am, the lowest values were 0.6%, 1.16 W, and 6.6 W, respectively. At 12:00 pm, the values of December are considered higher for several reasons, including the values of solar radiation and the ambient temperature are lower than those of June, as well as the difference in the angle of incidence of solar radiation, so the thermal energy required for heating is higher. The effectiveness was tested against pseudomonas aeruginosa bacteria and samples of surgical kits made of stainless steel, where the highest values of steam temperatures for sterilization were recorded between 122 °C to 132 °C. Under a steam pressure of 1.3 to 1.9 bar between 11:00 am to 3:00 pm, one sterilization cycle took 30 minutes in June 2022, and for the month December 2022 the highest values of steam temperatures for sterilization were between 101 °C to 117 °C. Under steam pressure 0.7 to 1.03 bar between the hours 10:30 am to 12:30 pm, where the sterilization cycle was between 40 to 60 minutes, as a result of the decrease in solar radiation.
建立并测试了太阳能高压釜,该高压釜由抛物面碟形反射器制成,可在焦点处收集太阳辐射以加热容器。通过热能效率方程以及蒸发水所需的总热能和所需热能,对 2022 年 6 月至 12 月的热性能进行了测试和比较,其中 2022 年 6 月上午 9 点的最高值分别为 9%、46 W 和 18 W。由于太阳辐射值较低,加热所需的能量较高,最低值分别为 0.2%、1.9 W 和 0.5 W,时间为中午 12:30 因为太阳辐射值较高,加热所需的能量较低。12 月 2022 日的最高值分别为 22%、23 W 和 14 W,而上午 9:00 的最低值分别为 0.6%、1.16 W 和 6.6 W。中午 12:00 时,12 月份的数值被认为较高,原因有几个,包括太阳辐射量和环境温度的数值低于 6 月份,以及太阳辐射量的入射角度不同,因此加热所需的热能较高。对铜绿假单胞菌和不锈钢制成的手术器械样本进行了效果测试,记录到的最高灭菌蒸汽温度值在 122 °C 至 132 °C 之间。2022 年 6 月,在上午 11:00 至下午 3:00 期间,蒸汽压力为 1.3 至 1.9 巴时,一个灭菌周期耗时 30 分钟;2022 年 12 月,灭菌蒸汽温度的最高值为 101 °C 至 117 °C。在蒸汽压力为 0.7 至 1.03 巴的情况下,上午 10:30 至中午 12:30,由于太阳辐射减少,灭菌周期为 40 至 60 分钟。
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引用次数: 0
The Role of Immune Check Point Gene Expression of Pd-1 and Tim-3 in Patients with Acute Myeloid Leukemia Pd-1 和 Tim-3 的免疫检查点基因表达在急性髓性白血病患者中的作用
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1418
Sara Mohammed Oudah, I. H. Hamzah, B. Matti
Acute myeloid leukemia (AML) is a hematopoietic cell cancer that spreads quickly to the blood and rapidly developing in the bone marrow. The prognosis for patients with acute myeloid leukemia (AML) is still poor, despite recent improvements in the therapeutic landscape. In hematological malignancies, immune checkpoint inhibitors have been studied, such as AML; however, the role of program cell death -1(PD-1) and T-cell immunoglobulin and mucin domain 3 (TIM3) in AML has not been thoroughly elucidated yet. Thus, the current study conducted to investigate the PD-1 and TIM‑3 gene expression in the AML patients and determine its associations with clinical outcomes and prognostic variables. The study collected 80 blood samples from acute myeloid leukemia (AML) patients and 40 blood samples from volunteer healthy individual were evaluated as control and real time quantitative (qRT-PCR) analysis was detect to performed PD-1 and TIM‑3 expression. The result showed there was non-significant (P>0.0001) in expression of TIM-3 in patients with AML, while expression of PD-1 statistically has high significant difference (P ≤ 0.0001). A cutoff value of PD-1 for patients vs. control was (0.853) with high sensitivity than cutoff value of TIM-3 for patients vs. control that can be diagnostically significant in distinguishing between patients and controls. Our data result showed that high expression of PD-1 in T cell is extremely correlated with progression of disease and down regulated gene expression of TIM-3 in AML patients.
急性髓性白血病(AML)是一种造血细胞癌,会迅速扩散到血液中,并在骨髓中迅速发展。尽管最近治疗手段有所改善,但急性髓性白血病(AML)患者的预后仍然很差。在血液恶性肿瘤中,免疫检查点抑制剂已被研究,如急性髓性白血病;然而,程序性细胞死亡-1(PD-1)和T细胞免疫球蛋白和粘蛋白结构域3(TIM3)在急性髓性白血病中的作用尚未被彻底阐明。因此,本研究旨在调查急性髓细胞性白血病患者的 PD-1 和 TIM-3 基因表达,并确定其与临床结果和预后变量的关系。研究收集了80份急性髓性白血病(AML)患者的血液样本,并评估了40份健康志愿者的血液样本作为对照,对PD-1和TIM-3的表达进行了实时定量(qRT-PCR)分析。结果显示,急性髓细胞白血病患者中 TIM-3 的表达无显著性差异(P>0.0001),而 PD-1 的表达在统计学上有高度显著性差异(P ≤ 0.0001)。患者与对照组的 PD-1 临界值(0.853)比患者与对照组的 TIM-3 临界值灵敏度高,在区分患者与对照组方面具有诊断意义。我们的数据结果表明,T 细胞中 PD-1 的高表达与急性髓细胞性白血病患者的疾病进展和 TIM-3 基因表达下调有极大的相关性。
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引用次数: 0
Unveiling the High Prevalence of Antibiotic Resistance and Quorum Sensing Genes in Uropathogenic Escherichia coli 揭示尿路致病性大肠杆菌的抗生素耐药性和法定量感应基因的高流行率
Pub Date : 2024-03-30 DOI: 10.23851/mjs.v35i1.1429
Hanan M. Abbas, Radhouane Gdoura, M. Al-Marjani
Escherichia coli is considered one of the uropathogenic bacteria with different infection symptoms representing mild illness to acute sepsis. This study aims to detect E. coli in patients with urinary infection and investigate quorum sensing genes (lux S and motA) in multi-drug resistant isolates of E. coli.  200 urine samples were collected from patients with urinary tract infections from several hospitals in Baghdad. The antibiotics sensitivity test showed high resistance of isolates for Ampicillin (100%), Cefazolin (97%), Trimethoprim/ Sulfamethoxazole (83%), Ceftriaxone (77%), Ceftazidime and Ciprofloxacin (70% each of them), and moderate resistance of isolates for Levofloxacin (50%), Gentamicin (47%), Cefepime (40%), while low resistance Piperacillin/ Tazobactam (33%), Cefoxitin (30%), Nitrofurantoin (17%), Imipenem (10%), Ertapenem and Amikacin (7% each of them), and Tigecycline (3%). The results showed an increase percentage of infection in females was 30% in the ages 30-44 years, whereas in ages 15-29 and more than 45 years was 17%.  There was a high percentage (57.11%) of resistant isolates in females which are ages 30-44 years. While the ages more than 45 years were 66.4% and ages 15-29 were 34%. While, in males, the percentage was high in ages more than 45 years (35.25%) followed by age groups 30-44 years (31.5%) and 15-29 years (31%).  The prevalence percentage for luxS and motA genes in E. coli was 100%. In conclusion, E. coli isolates were multi-drug resistant due to all isolates had quorum sensing genes. Moreover, uropathogenic of E. coli in females was more frequent than in males due to the resistance of bacteria to antibiotics.   
大肠杆菌被认为是泌尿系统致病菌之一,其感染症状从轻微疾病到急性败血症不等。本研究旨在检测泌尿系统感染患者体内的大肠杆菌,并调查耐多药大肠杆菌分离株中的法定量感应基因(lux S 和 motA)。 研究人员从巴格达的几家医院收集了 200 份尿路感染患者的尿液样本。抗生素敏感性测试表明,分离菌株对氨苄西林(100%)、头孢唑啉(97%)、甲氧苄啶/磺胺甲噁唑(83%)、头孢曲松(77%)、头孢唑肟和环丙沙星(各 70%)具有高度耐药性、而对哌拉西林/他唑巴坦(33%)、头孢西丁(30%)、硝基呋喃妥因(17%)、亚胺培南(10%)、厄他培南和阿米卡星(各 7%)以及替加环素(3%)的耐药性较低。结果显示,30-44 岁女性感染率增加了 30%,而 15-29 岁和 45 岁以上女性感染率为 17%。 在 30-44 岁的女性中,耐药分离株的比例很高(57.11%)。而 45 岁以上的比例为 66.4%,15-29 岁的比例为 34%。男性中,45岁以上年龄组的比例较高(35.25%),其次是30-44岁年龄组(31.5%)和15-29岁年龄组(31%)。 大肠杆菌中luxS和motA基因的流行率为100%。总之,由于所有分离菌株都具有法定人数感应基因,因此大肠杆菌具有多重耐药性。此外,由于细菌对抗生素具有抗药性,女性感染尿路致病性大肠杆菌的频率高于男性。
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Al-Mustansiriyah Journal of Science
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