Analytical Method: Determination of famotidine drug using chemiluminescence method

Abstract

This study involved the development of a novel, cost-effective, fast, and highly sensitive analytical technique for quantifying minimal amounts of the drug famotidine through chemiluminescence. The method is centred around the measurement of energy emitted as a result of the interaction between the drug and Luminol in an alkaline solution; this interaction generates an electronically excited intermediate state, releasing a portion of the system’s energy as photons. The method was sensitive for the analysis of famotidine. The linear calibration curve (LR) is obtained in the range 2-12 mg mL-1, with a high correlation coefficient (R2) of 0.9929. The molecular absorption coefficient  (ε) was calculated at 2621×104 L mol-1 cm-1. The method displayed excellent sensitivity with a Sandell’s sensitivity of 1.287×10-5 mg cm-2, the detection limit (LOD) was found to be 0.0314 mg mL-1, and the limit of quantification (LOQ) was 0.0952 mg mL-1. This study found that recovery was obtained at 104 - 96.5 %, and the relative standard deviation (RSD%) was below 1.981%. The results showed that the proposed technique has efficient recovery for measuring famotidine in pharmaceutical preparations.