Stimulation of the Spinal Cord of Decerebrated Rat with Double Pulses

IF 0.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of Evolutionary Biochemistry and Physiology Pub Date : 2024-04-26 DOI:10.1134/s0022093024020054

V. A. Lyakhovetskii, P. Yu. Shkorbatova, O. V. Gorsky, N. V. Pavlova, E. Yu. Bazhenova, P. E. Musienko, N. S. Merkulyeva

{"title":"Stimulation of the Spinal Cord of Decerebrated Rat with Double Pulses","authors":"V. A. Lyakhovetskii, P. Yu. Shkorbatova, O. V. Gorsky, N. V. Pavlova, E. Yu. Bazhenova, P. E. Musienko, N. S. Merkulyeva","doi":"10.1134/s0022093024020054","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Analysis of responses on electrical stimulation is one of\nthe experimental paradigms to study the excitability of the nervous\nsystem. In particular, the technique of recording muscle responses\nevoked by electrical epidural stimulation (ES) of the spinal cord\n(SC) in humans and animals is widely used. In rats decerebrated\nat the precollicular level, responses of mm.\ntibialis anterior (TA) and gastrocnemius medialis (GM)\non ES of the L2, L4, L6 spinal segments and transvertebral stimulation\n(TS) of the VL2, VL4, VL6 vertebrae with single and double pulses\nwere analyzed. The currents at which the amplitude of the sensory\ncomponent of the response for a single pulse and one of the pulses\nof the pair was maximum were determined. At the minimum of these\ncurrents, the ratio of the amplitudes of the sensory component of\nthe response to the first and second pulses to the amplitude of\nthe sensory component of the response to a single pulse was analyzed.\nFor both muscles, a weakening of the response to both pulses of\nthe pair was obtained with TS VL2 and VL4, while when stimulating\nVL2, the TA response to the second pulse was lower than to the first.\nOn the contrary, with ES of all segments of interest, a facilitation of\nthe response to the second pulse was obtained for both muscles.\nA similar facilitation was qualitatively observed for two other\nmuscles, mm. iliacus and vastus lateralis. Thus, the use of\ndouble pulses during stimulation made it possible to identify the\ndependence of the response of SC neural networks on the method of\ntheir activation (TS or ES). The facilitation of the response to\nthe second pulse during ES is presumably explained by a decrease\nin presynaptic inhibition due to decerebration.","PeriodicalId":15805,"journal":{"name":"Journal of Evolutionary Biochemistry and Physiology","volume":"90 1","pages":""},"PeriodicalIF":0.6000,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Evolutionary Biochemistry and Physiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024020054","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Analysis of responses on electrical stimulation is one of the experimental paradigms to study the excitability of the nervous system. In particular, the technique of recording muscle responses evoked by electrical epidural stimulation (ES) of the spinal cord (SC) in humans and animals is widely used. In rats decerebrated at the precollicular level, responses of mm. tibialis anterior (TA) and gastrocnemius medialis (GM) on ES of the L2, L4, L6 spinal segments and transvertebral stimulation (TS) of the VL2, VL4, VL6 vertebrae with single and double pulses were analyzed. The currents at which the amplitude of the sensory component of the response for a single pulse and one of the pulses of the pair was maximum were determined. At the minimum of these currents, the ratio of the amplitudes of the sensory component of the response to the first and second pulses to the amplitude of the sensory component of the response to a single pulse was analyzed. For both muscles, a weakening of the response to both pulses of the pair was obtained with TS VL2 and VL4, while when stimulating VL2, the TA response to the second pulse was lower than to the first. On the contrary, with ES of all segments of interest, a facilitation of the response to the second pulse was obtained for both muscles. A similar facilitation was qualitatively observed for two other muscles, mm. iliacus and vastus lateralis. Thus, the use of double pulses during stimulation made it possible to identify the dependence of the response of SC neural networks on the method of their activation (TS or ES). The facilitation of the response to the second pulse during ES is presumably explained by a decrease in presynaptic inhibition due to decerebration.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

用双脉冲刺激脱脑大鼠的脊髓

摘要分析电刺激反应是研究神经系统兴奋性的实验范例之一。特别是记录人和动物脊髓硬膜外电刺激（ES）引起的肌肉反应的技术被广泛使用。在蝶骨前水平去脑化的大鼠身上，分析了胫骨前肌（TA）和腓肠肌内侧肌（GM）对 L2、L4、L6 脊柱节段 ES 和 VL2、VL4、VL6 椎体跨椎刺激（TS）单双脉冲的反应。确定了单脉冲和双脉冲中一个脉冲响应的感觉部分振幅达到最大值时的电流。在这些电流的最小值处，分析了对第一和第二个脉冲的反应的感觉分量的振幅与对单个脉冲的反应的感觉分量的振幅的比值。对这两块肌肉而言，当刺激 TS VL2 和 VL4 时，对双脉冲中的两个脉冲的反应都减弱了，而当刺激 VL2 时，TA 对第二个脉冲的反应低于对第一个脉冲的反应。相反，在刺激所有相关节段的 ES 时，两块肌肉对第二个脉冲的反应都有促进作用。因此，在刺激过程中使用双脉冲可以确定 SC 神经网络的反应取决于其激活方法（TS 或 ES）。ES时对第二个脉冲反应的促进作用可能是由于去脑化导致突触前抑制作用减弱。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Journal of Evolutionary Biochemistry and Physiology 生物-进化生物学

自引率

33.30%

发文量

110

审稿时长

6-12 weeks

期刊介绍： Journal of Evolutionary Biochemistry and Physiology publishes original experimental and theoretical and review articles related to evolution of the main forms of metabolism in connection with life origin; comparative and ontogenetic physiology and biochemistry, biochemical evolution of animal world; as well as evolution of functions; morphology, pharmacology, pathophysiology and ecological physiology. The journal welcomes manuscripts from all countries in the English or Russian language.