A Comparative Analysis of Erythrocyte Osmotic Fragility across Vertebrate Taxa

Pub Date : 2024-08-29 DOI:10.1134/s0022093024040094

B. A. Gerda, E. A. Skverchinskaya, A. Yu. Andreeva, A. A. Volkova, S. Gambaryan, I. V. Mindukshev

{"title":"A Comparative Analysis of Erythrocyte Osmotic Fragility across Vertebrate Taxa","authors":"B. A. Gerda, E. A. Skverchinskaya, A. Yu. Andreeva, A. A. Volkova, S. Gambaryan, I. V. Mindukshev","doi":"10.1134/s0022093024040094","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The osmotic fragility of erythrocytes is a parameter that\nreflects the ability of cells to endure variations in the osmotic\nenvironment. The impairment of this ability is often associated\nwith a variety of pathologies, encompassing hemolytic anemias, malignant\ntumors, and cardiovascular dysfunctions. Osmotic fragility exhibits\nvariability across different animal taxa and is closely related\nwith ecosystems. We developed the method for assessing osmotic fragility\nusing a laser particle size analyzer, which facilitates real-time\nkinetic monitoring of cell concentration changes under controlled\ntemperature conditions. The species examined included Homo sapiens, Rattus\nnorvegicus domestica, Coturnix japonica\ndomestica, Rana ridibunda, Carassius carassius, and Lampetra fluviatilis. The methodology\nwas presented in two variants: (1) manual water additions and (2)\nautomated medium dilution. The key parameters characterizing osmotic\nfragility included H\n50 (the\nosmolality that lysed half of lysis-susceptible cells), H\n90 (90% cell\nlysis), and W (population heterogeneity\nby the degree of lysis resistance). In terms of H\n50 and W, the results obtained via this\nmethod did not differ significantly from those obtained by spectrophotometry\nand flow cytometry. No significant differences were also observed between\nthe outcomes of automated and manual variants of the method. Erythrocytes\nof aquatic and semiaquatic animals exhibited significantly higher\nresistance to hypotonic lysis. Among all species examined, amphibian\n(Rana ridibunda) and lamprey\n(Lampetra fluviatilis) erythrocytes\ndemonstrated the lowest osmotic fragility. The most pronounced variability\nin the degree of lysis resistance was detected among amphibians,\nwith an almost twofold difference compared to other taxa examined.\nWhile mammalian (human and rat) erythrocytes exhibited similar fragility\nlevels, they were more variable in their resistance profiles. Avian\nerythrocytes demonstrated a half-lysis occurrence at higher osmolality levels\ncompared to mammalian erythrocytes, although in the domestic quail\n(Coturnix japonica domestica),\nerythrocytes lysed over a considerably wider osmotic range and contained\na subset of cells resistant to hypotonic lysis. These findings indicate\nthat erythrocytes of lower vertebrates share a lower osmotic fragility\ncompared to those of higher vertebrates, a phenomenon most likely\nattributable to embryonic characteristics, ecto-/endothermy, and\nhabitat considerations.","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1134/s0022093024040094","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

The osmotic fragility of erythrocytes is a parameter that reflects the ability of cells to endure variations in the osmotic environment. The impairment of this ability is often associated with a variety of pathologies, encompassing hemolytic anemias, malignant tumors, and cardiovascular dysfunctions. Osmotic fragility exhibits variability across different animal taxa and is closely related with ecosystems. We developed the method for assessing osmotic fragility using a laser particle size analyzer, which facilitates real-time kinetic monitoring of cell concentration changes under controlled temperature conditions. The species examined included Homo sapiens, Rattus norvegicus domestica, Coturnix japonica domestica, Rana ridibunda, Carassius carassius, and Lampetra fluviatilis. The methodology was presented in two variants: (1) manual water additions and (2) automated medium dilution. The key parameters characterizing osmotic fragility included H ₅₀ (the osmolality that lysed half of lysis-susceptible cells), H ₉₀ (90% cell lysis), and W (population heterogeneity by the degree of lysis resistance). In terms of H ₅₀ and W, the results obtained via this method did not differ significantly from those obtained by spectrophotometry and flow cytometry. No significant differences were also observed between the outcomes of automated and manual variants of the method. Erythrocytes of aquatic and semiaquatic animals exhibited significantly higher resistance to hypotonic lysis. Among all species examined, amphibian (Rana ridibunda) and lamprey (Lampetra fluviatilis) erythrocytes demonstrated the lowest osmotic fragility. The most pronounced variability in the degree of lysis resistance was detected among amphibians, with an almost twofold difference compared to other taxa examined. While mammalian (human and rat) erythrocytes exhibited similar fragility levels, they were more variable in their resistance profiles. Avian erythrocytes demonstrated a half-lysis occurrence at higher osmolality levels compared to mammalian erythrocytes, although in the domestic quail (Coturnix japonica domestica), erythrocytes lysed over a considerably wider osmotic range and contained a subset of cells resistant to hypotonic lysis. These findings indicate that erythrocytes of lower vertebrates share a lower osmotic fragility compared to those of higher vertebrates, a phenomenon most likely attributable to embryonic characteristics, ecto-/endothermy, and habitat considerations.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

脊椎动物类群红细胞渗透脆性的比较分析

摘要红细胞的渗透脆性是反映细胞承受渗透环境变化能力的一个参数。这种能力的损害通常与多种病症有关，包括溶血性贫血、恶性肿瘤和心血管功能障碍。渗透脆性在不同动物类群中表现出差异性，并与生态系统密切相关。我们开发了利用激光粒度分析仪评估渗透脆性的方法，这种分析仪便于在受控温度条件下对细胞浓度变化进行实时动态监测。所研究的物种包括智人（Homo sapiens）、家养鼠（Rattusnorvegicus domestica）、日本豚鼠（Coturnix japonicadomestica）、秧鸡（Rana ridibunda）、鲫鱼（Carassius carassius）和野鸭（Lampetra fluviatilis）。该方法有两种变体：(1) 手动加水；(2) 自动培养基稀释。表征渗透脆性的关键参数包括 H50（溶解一半易溶细胞的渗透压）、H90（溶解 90% 的细胞）和 W（根据抗溶解度确定的种群异质性）。就 H50 和 W 而言，该方法得出的结果与分光光度法和流式细胞术得出的结果没有显著差异。自动和手动方法的结果也无明显差异。水生和半水生动物的红细胞对低渗裂解的抵抗力明显较高。在所有检测物种中，两栖动物（Rana ridibunda）和鳗鱼（Lampetra fluviatilis）的红细胞表现出最低的渗透脆性。虽然哺乳动物（人和大鼠）的红细胞表现出相似的脆性水平，但它们的抗溶解性差异更大。与哺乳动物红细胞相比，禽类红细胞在较高的渗透压水平下会出现半溶解现象，但在家养鹌鹑（Coturnix japonica domestica）中，红细胞的溶解渗透压范围要宽得多，而且有一部分细胞对低渗溶解具有抵抗力。这些发现表明，与高等脊椎动物相比，低等脊椎动物的红细胞具有较低的渗透脆性，这种现象很可能与胚胎特征、体外/体内温度以及栖息地有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助