Assessment of Left Lung Remodeling With Magnetic Resonance Imaging in a Murine Model Following Exposure to Douglas Fir Smoke.

IF 1.7 4区 医学 Q4 BIOPHYSICS Journal of Biomechanical Engineering-Transactions of the Asme Pub Date : 2024-07-01 DOI:10.1115/1.4065272
Jacqueline Matz, Mireia Perera Gonzalez, Peter Niedbalski, Hannah Kim, Ye Chen, Paola Sebastiani, Michael J Gollner, Chiara Bellini, Jessica M Oakes
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Abstract

Wildland firefighters (WLFFs) experience lung function decline due to occupational exposure to fire smoke. WLFFs typically do not wear respiratory personal protective equipment, and if they do, it is a simple bandana, which is not effective at filtering smoke. To pinpoint the biological underpinnings of abnormal respiratory function following 3-7 years of WLFF service, we exposed mice to Douglas fir smoke (DFS) over 8 weeks. Following exposure, we assessed changes in lung structure through Magnetic Resonance Imaging (MRI) and histological analysis, which was supported by immunohistochemistry staining. With MRI, we found that the signal decay time, T2*, from ultrashort echo time (UTE) images was significantly shorter in mice exposed to DFS compared to air controls. In addition, the variation in T2* was more heterogeneously distributed throughout the left lung in DFS-exposed mice, compared to air controls. As confirmed by histological analysis, shorter T2* was caused by larger parenchyma airspace sizes and not fibrotic remodeling. Destruction of the alveolar spaces was likely due to inflammation, as measured by an influx of CD68+ macrophages and destruction due to enhanced neutrophil elastase. In addition, measurements of airspace dimensions from histology were more heterogeneously distributed throughout the lung, corroborating the enhanced relative dispersion of T2*. Findings from this study suggest that the decline in lung function observed in WLFFs may be due to emphysema-like changes in the lung, which can be quantified with MRI.

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通过磁共振成像评估暴露于花旗松烟雾后小鼠模型的左肺重塑情况
野外消防员(WLFFs)因职业原因暴露于火灾烟雾中,会导致肺功能下降。野地消防员通常不穿戴呼吸道个人防护装备,即使穿戴,也只是简单的头巾,无法有效过滤烟雾。为了准确找出 WLFF 工作 3-7 年后呼吸功能异常的生物学基础,我们将小鼠暴露在花旗松烟(DFS)中长达 8 周。暴露后,我们通过核磁共振成像(MRI)和组织学分析评估了肺部结构的变化,并通过免疫组化染色对此进行了支持。通过核磁共振成像,我们发现与空气对照组相比,暴露于DFS的小鼠超短回波时间(UTE)图像的信号衰减时间(T2*)明显缩短。此外,与空气对照组相比,暴露于 DFS 的小鼠整个左肺的 T2* 变化更加不均匀。组织学分析证实,T2*变短是由于肺实质气腔变大而不是纤维重塑造成的。肺泡间隙的破坏很可能是由于炎症引起的,如 CD68+ 巨噬细胞的涌入和中性粒细胞弹性蛋白酶增强引起的破坏。此外,组织学测量的气腔尺寸在整个肺部的分布更加不均匀,这也证实了 T2* 的相对分散性增强。本研究的结果表明,在WLFFs中观察到的肺功能下降可能是由于肺气肿样改变引起的,这可以通过核磁共振成像进行量化。
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来源期刊
CiteScore
3.40
自引率
5.90%
发文量
169
审稿时长
4-8 weeks
期刊介绍: Artificial Organs and Prostheses; Bioinstrumentation and Measurements; Bioheat Transfer; Biomaterials; Biomechanics; Bioprocess Engineering; Cellular Mechanics; Design and Control of Biological Systems; Physiological Systems.
期刊最新文献
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