Comparative physiological and transcriptomic analysis of sono-biochemical control over post-acidification of Lactobacillus delbrueckii subsp. bulgaricus

IF 4.5 1区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Food microbiology Pub Date : 2024-05-18 DOI:10.1016/j.fm.2024.104563
Xiaohui Zhang , Yuanrong Zheng , Changyu Zhou , Jinxuan Cao , Daodong Pan , Zhendong Cai , Zhen Wu , Qiang Xia
{"title":"Comparative physiological and transcriptomic analysis of sono-biochemical control over post-acidification of Lactobacillus delbrueckii subsp. bulgaricus","authors":"Xiaohui Zhang ,&nbsp;Yuanrong Zheng ,&nbsp;Changyu Zhou ,&nbsp;Jinxuan Cao ,&nbsp;Daodong Pan ,&nbsp;Zhendong Cai ,&nbsp;Zhen Wu ,&nbsp;Qiang Xia","doi":"10.1016/j.fm.2024.104563","DOIUrl":null,"url":null,"abstract":"<div><p>Thermosonication (UT) prestress treatments combining with varied fermentation patterns has been revealed as an effective method to regulate post-acidification as exerted by <em>Lactobacillus delbrueckii</em> subsp. <em>bulgaricus</em> (<em>L. delbrueckii</em>), but sono-biochemical controlling mechanisms remain elusive. This study employed physiological and transcriptomic analysis to explore the response mechanism of <em>L. delbrueckii</em> to UT-induced microstress (600 W, 33 kHz, 10 min). UT stress-induced inhibition of acidification of <em>L. delbrueckii</em> during (post)-fermentation was first confirmed, relying on the UT process parameters such as stress exposure duration and UT power. The significantly enhanced membrane permeability in cells treated by 600 W for 10 min than the microbes stressed by 420 W for 20 min suggested the higher dependence of UT-derived stresses on the treatment durations, relative to the ultrasonic powers. In addition, ultrasonication treatment-induced changes in cell membrane integrity enhanced and/or disrupted permeability of <em>L. delbrueckii</em>, resulting in an imbalance in intracellular conditions associated with corresponding alterations in metabolic behaviors and fermentation efficiencies. UT-prestressed inoculum exhibited a 21.46% decrease in the membrane potential during the lag phase compared to untreated samples, with an intracellular pH of 5.68 ± 0.12, attributed to the lower activities of H<sup>+</sup>-ATPase and lactate dehydrogenase due to UT stress pretreatments. Comparative transcriptomic analysis revealed that UT prestress influenced the genes related to glycolysis, pyruvate metabolism, fatty acid synthesis, and ABC transport. The genes encoding 3-oxoacyl-[acyl-carrier-protein] reductases I, II, and III, CoA carboxylase, lactate dehydrogenase, pyruvate oxidase, glucose-6-phosphate isomerase, and glycerol-3-phosphate dehydrogenase were downregulated, thus identifying the relevance of the UT microstresses-downregulated absorption and utilization of carbohydrates with the attenuated fatty acid production and energy metabolisms. These findings could contribute to provide a better understanding of the inactivated effects on the post-acidification of <em>L. delbrueckii</em> by ultrasonic pretreatments, thus providing theoretical basis for the targeted optimization of acidification inhibition efficiencies for yogurt products during chilled preservation processes.</p></div>","PeriodicalId":12399,"journal":{"name":"Food microbiology","volume":null,"pages":null},"PeriodicalIF":4.5000,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0740002024001011","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Thermosonication (UT) prestress treatments combining with varied fermentation patterns has been revealed as an effective method to regulate post-acidification as exerted by Lactobacillus delbrueckii subsp. bulgaricus (L. delbrueckii), but sono-biochemical controlling mechanisms remain elusive. This study employed physiological and transcriptomic analysis to explore the response mechanism of L. delbrueckii to UT-induced microstress (600 W, 33 kHz, 10 min). UT stress-induced inhibition of acidification of L. delbrueckii during (post)-fermentation was first confirmed, relying on the UT process parameters such as stress exposure duration and UT power. The significantly enhanced membrane permeability in cells treated by 600 W for 10 min than the microbes stressed by 420 W for 20 min suggested the higher dependence of UT-derived stresses on the treatment durations, relative to the ultrasonic powers. In addition, ultrasonication treatment-induced changes in cell membrane integrity enhanced and/or disrupted permeability of L. delbrueckii, resulting in an imbalance in intracellular conditions associated with corresponding alterations in metabolic behaviors and fermentation efficiencies. UT-prestressed inoculum exhibited a 21.46% decrease in the membrane potential during the lag phase compared to untreated samples, with an intracellular pH of 5.68 ± 0.12, attributed to the lower activities of H+-ATPase and lactate dehydrogenase due to UT stress pretreatments. Comparative transcriptomic analysis revealed that UT prestress influenced the genes related to glycolysis, pyruvate metabolism, fatty acid synthesis, and ABC transport. The genes encoding 3-oxoacyl-[acyl-carrier-protein] reductases I, II, and III, CoA carboxylase, lactate dehydrogenase, pyruvate oxidase, glucose-6-phosphate isomerase, and glycerol-3-phosphate dehydrogenase were downregulated, thus identifying the relevance of the UT microstresses-downregulated absorption and utilization of carbohydrates with the attenuated fatty acid production and energy metabolisms. These findings could contribute to provide a better understanding of the inactivated effects on the post-acidification of L. delbrueckii by ultrasonic pretreatments, thus providing theoretical basis for the targeted optimization of acidification inhibition efficiencies for yogurt products during chilled preservation processes.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
超声生化控制保加利亚乳杆菌后酸化的生理学和转录组学比较分析
热声波(UT)预应力处理与不同的发酵模式相结合,已被认为是调节保加利亚乳杆菌(L. delbrueckii subsp. bulgaricus,L. delbrueckii)后酸化的一种有效方法,但声波生物化学控制机制仍然难以捉摸。本研究采用生理学和转录组学分析,探讨了UT诱导的微应激(600瓦、33千赫、10分钟)对德尔布鲁贝克乳杆菌的响应机制。根据UT过程参数,如应激暴露持续时间和UT功率,首次证实了UT应激诱导抑制了发酵(后)过程中的L. delbrueckii酸化。经 600 瓦 10 分钟处理的细胞膜渗透性明显高于经 420 瓦 20 分钟处理的微生物,这表明相对于超声功率而言,UT 衍生应力对处理持续时间的依赖性更高。此外,超声波处理引起的细胞膜完整性变化增强和/或破坏了德尔布鲁贝克氏菌的通透性,导致细胞内条件失衡,代谢行为和发酵效率也随之改变。与未经处理的样品相比,UT胁迫接种物在滞后期的膜电位下降了21.46%,细胞内pH值为5.68 ± 0.12,这归因于UT胁迫预处理导致H+-ATP酶和乳酸脱氢酶活性降低。转录组比较分析表明,UT预胁迫影响了与糖酵解、丙酮酸代谢、脂肪酸合成和ABC转运相关的基因。编码 3-氧代酰基-[酰基载体蛋白]还原酶 I、II 和 III、CoA 羧化酶、乳酸脱氢酶、丙酮酸氧化酶、葡萄糖-6-磷酸异构酶和甘油-3-磷酸脱氢酶的基因被下调,从而确定了UT 微应激下调碳水化合物的吸收和利用与脂肪酸生成和能量代谢减弱的相关性。这些发现有助于更好地理解超声波预处理对三角酪蛋白酸化后的失活效应,从而为在冷藏保存过程中有针对性地优化酸奶产品的酸化抑制效率提供理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Food microbiology
Food microbiology 工程技术-生物工程与应用微生物
CiteScore
11.30
自引率
3.80%
发文量
179
审稿时长
44 days
期刊介绍: Food Microbiology publishes original research articles, short communications, review papers, letters, news items and book reviews dealing with all aspects of the microbiology of foods. The editors aim to publish manuscripts of the highest quality which are both relevant and applicable to the broad field covered by the journal. Studies must be novel, have a clear connection to food microbiology, and be of general interest to the international community of food microbiologists. The editors make every effort to ensure rapid and fair reviews, resulting in timely publication of accepted manuscripts.
期刊最新文献
Current state, challenges and future orientations of the applications of lactic acid bacteria exopolysaccharide in foods pLM33 provides tolerance of persistent Listeria monocytogenes ST5 to various stress conditions and also enhances its virulence Editorial Board Improving ready-to-eat meat safety: Evaluating the bacterial-inactivation efficacy of microplasma-based far-UVC light treatment of food-contact surfaces and deli turkey breast Using plasma-activated water for decontamination of Salmonella spp. on common building surfaces in poultry houses
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1