Psidium guajav-mediated zinc oxide nanoparticles as a multifunctional, microbicidal, antioxidant and antiproliferative agent against destructive pathogens.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-09-01 Epub Date: 2024-06-27 DOI:10.1007/s00449-024-03052-x
P Prapula Thejashwini, R Chandrika, M C Madhusudhan, Shreya M Joshi, Daoud Ali, Saud Alarifi, Sudisha Jogaiah, Nagaraja Geetha
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Abstract

Bio-inspired zinc oxide nanoparticles are gaining immense interest due to their safety, low cost, biocompatibility, and broad biological properties. In recent years, much research has been focused on plant-based nanoparticles, mainly for their eco-friendly, facile, and non-toxic character. Hence, the current study emphasized a bottom-up synthesis of zinc oxide nanoparticles (ZnO NPs) from Psidium guajava aqueous leaf extract and evaluation of its biological properties. The structural characteristic features of biosynthesized ZnO NPs were confirmed using various analytical methods, such as UV-Vis spectroscopy, X-ray diffraction (XRD), energy-dispersive X-ray analysis (EDX), Fourier transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), Scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HR-TEM). The synthesized ZnO NPs exhibited a hydrodynamic shape with an average particle size of 11.6-80.2 nm. A significant antimicrobial efficiency with minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of 40 and 27 µg/ml for Enterococcus faecalis, followed by 30 and 40 µg/ml for Staphylococcus aureus, 20 and 30 µg/ml for Staphylococcus mutans, 30 µg/ml for Candida albicans was observed by ZnO NPs. Additionally, they showed significant breakdown of biofilms of Streptococcus mutans and Candida albicans indicating their future value in drug-resistance research. Furthermore, an excellent dose-dependent activity of antioxidant property was noticed with an IC50 of 9.89 µg/ml. The antiproliferative potential of the ZnO NPs was indicated by the viability of MDA MB 231 cells, which showed a drastic decrease in response to increased concentrations of biosynthesized ZnO NPs. Thus, the present results open up vistas to explore their pharmaceutical potential for the development of targeted anticancer drugs in the future.

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瓜子黄酮介导的纳米氧化锌颗粒是一种多功能杀菌、抗氧化和抗增殖剂,可对抗破坏性病原体。
生物启发的氧化锌纳米粒子因其安全、低成本、生物相容性和广泛的生物特性而备受关注。近年来,许多研究都集中在植物基纳米粒子上,主要是因为它们具有环保、简便和无毒的特点。因此,本研究强调从番石榴叶水提取物中自下而上合成氧化锌纳米粒子(ZnO NPs),并评估其生物特性。本研究采用多种分析方法,如紫外可见光谱、X射线衍射(XRD)、能量色散X射线分析(EDX)、傅立叶变换红外光谱(FT-IR)、动态光散射(DLS)、扫描电子显微镜(SEM)和高分辨率透射电子显微镜(HR-TEM),证实了生物合成的氧化锌纳米粒子的结构特征。合成的 ZnO NPs 呈流体力学形状,平均粒径为 11.6-80.2 nm。氧化锌氮氧化物具有明显的抗菌效果,对粪肠球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别为 40 微克/毫升和 27 微克/毫升,对金黄色葡萄球菌的抑菌浓度分别为 30 微克/毫升和 40 微克/毫升,对变异葡萄球菌的抑菌浓度分别为 20 微克/毫升和 30 微克/毫升,对白色念珠菌的抑菌浓度分别为 30 微克/毫升。此外,氧化锌纳米粒子对变异葡萄球菌和白色念珠菌的生物膜也有明显的破坏作用,这表明了氧化锌纳米粒子在耐药性研究中的未来价值。此外,它们还具有出色的剂量依赖性抗氧化活性,其 IC50 值为 9.89 µg/ml。氧化锌纳米粒子的抗增殖潜力体现在 MDA MB 231 细胞的存活率上,随着生物合成氧化锌纳米粒子浓度的增加,细胞存活率急剧下降。因此,目前的研究结果为今后探索其制药潜力、开发靶向抗癌药物开辟了前景。
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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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