A novel cost-effective methodology for the screening of nanocellulose producing micro-organisms.

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Bioprocess and Biosystems Engineering Pub Date : 2024-09-01 Epub Date: 2024-07-09 DOI:10.1007/s00449-024-03049-6
Moniya Katyal, Rakshanda Singh, Ritu Mahajan, Anurekha Sharma, Ranjan Gupta, Neeraj K Aggarwal, Anita Yadav
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Abstract

In this paper, the work has been done to develop a cost-effective methodology, for the isolation of the potential producer of bacterial nanocellulose. No report is available in the literature, on the use of gram flour and table sugar for the screening of nanocellulose-producing isolates. Since commercially used, Hestrin-Schramm medium is expensive for the isolation of nanocellulose-producing micro-organisms, the possibility of using gram flour-table sugar medium was investigated in this work. Qualitative screening of micro-organisms was done using cost-effective medium, i.e., gram flour-table sugar medium. Qualitative analysis of various nanocellulose-producing bacteria depicted that cellulose layer production occurred on both HS medium and gram flour-table sugar medium. The yield of nanocellulose was also better on air-liquid surface in case of gram flour-table sugar medium as compared to HS medium. 16S rRNA was used for molecular characterization of bacterial strain and the best nanocellulose producer was identified as Novacetimonas hansenii BMK-3_NC240423 (isolated from rotten banana). FTIR and FE-SEM studies of nanocellulose pellicle produced on HS medium and gram flour-table sugar medium demonstrated equivalent structural, morphological, and chemical properties. The cost of newly designed medium (0.01967 $/L) is nearly 90 times lower than the Hestrin-Schramm medium (1.748 $/L), which makes the screening of nanocellulose producers very cost-effective. A strategy of using gram flour extract-table sugar medium for the screening of nanocellulose-producing micro-organisms is a novel approach, which will drastically reduce the screening associated cost of cellulose-producing micro-organisms and also motivate the researchers/industries for comprehensive screening programme for getting high cellulose-producing microbes.

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筛选纳米纤维素生产微生物的经济有效的新方法。
本文旨在开发一种具有成本效益的方法,用于分离潜在的纳米纤维素细菌生产者。文献中没有关于使用克粉和食糖筛选纳米纤维素生产分离物的报道。由于市售的 Hestrin-Schramm 培养基价格昂贵,无法用于分离纳米纤维素生产微生物,因此本工作研究了使用克面粉-食糖培养基的可能性。使用经济有效的培养基,即克面粉-食糖培养基,对微生物进行了定性筛选。对各种纳米纤维素生产菌的定性分析表明,纤维素层的生产既发生在 HS 培养基上,也发生在克面粉-食糖培养基上。与 HS 培养基相比,克面粉-绵白糖培养基在空气-液体表面的纳米纤维素产量也更高。采用 16S rRNA 对细菌菌株进行分子鉴定,结果表明最好的纳米纤维素生产者是 Novacetimonas hansenii BMK-3_NC240423(从烂香蕉中分离出来)。傅立叶变换红外光谱(FTIR)和傅立叶变换扫描电子显微镜(FE-SEM)对在 HS 培养基和克粉-蔗糖培养基上产生的纳米纤维素胶粒进行了研究,结果表明其结构、形态和化学特性相当。新设计的培养基成本(0.01967 美元/升)比 Hestrin-Schramm 培养基(1.748 美元/升)低近 90 倍,使纳米纤维素生产者的筛选非常经济有效。使用克面粉提取物-表糖培养基来筛选纳米纤维素生产微生物的策略是一种新方法,它将大大降低纤维素生产微生物的筛选相关成本,同时也激励研究人员/工业界开展全面的筛选计划,以获得高纤维素生产微生物。
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来源期刊
Bioprocess and Biosystems Engineering
Bioprocess and Biosystems Engineering 工程技术-工程:化工
CiteScore
7.90
自引率
2.60%
发文量
147
审稿时长
2.6 months
期刊介绍: Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.
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