Apple Pomace Extract Induces Cell Proliferation and Increases Type I Collagen and Hyaluronan Production in Human Skin Fibroblasts In Vitro.

IF 3.1 2区 农林科学 Q2 CHEMISTRY, APPLIED Plant Foods for Human Nutrition Pub Date : 2024-09-01 Epub Date: 2024-07-13 DOI:10.1007/s11130-024-01210-w
Naoki Nanashima, Hayato Maeda, Akira Nakajima, Makoto Nishizuka, Tsuyoshi Narumi, Junji Ichita, Koh Itoku
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Abstract

Apple pomace is the residue left after apples are squeezed. The majority of pomace produced worldwide is produced by the apple manufacturing industry, however, most of the pomace produced by the industry is discarded. Apple pomace contains functional ingredients, such as polyphenols and triterpenoids, and exerts several beneficial effects on human health; however, studies on its cosmetic effects on the skin are lacking. Therefore, herein, we investigated the effects of apple pomace extract (APE) on human skin fibroblasts (HSFs) in vitro. When HSFs were cultured with the extract for 72 h, the number of HSFs increased at concentrations of 10 and 20 µg/mL. Transcriptome analysis and reverse transcription-quantitative PCR results revealed that the extract upregulated the expression of hyaluronan synthase (HAS) 1, HAS2, and HAS3 and downregulated the expression of HYAL1, a gene encoding the hyaluronan-degrading enzyme, in HSFs. Additionally, enzyme-linked immunosorbent assay revealed increased amounts of factors related to skin extracellular matrix, such as type I collagen and hyaluronic acid, secreted in the culture supernatant. The western blotting results suggested that the extract induced extracellular signal-regulated kinase and protein kinase B phosphorylation in HSFs. Additionally, several GO_Terms related to mitosis were detected in the Gene Ontology analysis. This is the first study to show that APE induces the proliferation of HSFs and production of factors related to skin anti-aging.

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苹果渣提取物可诱导细胞增殖,并增加体外人体皮肤成纤维细胞中 I 型胶原蛋白和透明质酸的生成。
苹果渣是苹果榨汁后留下的残渣。全世界生产的大部分果渣由苹果制造业生产,但该行业生产的大部分果渣都被丢弃。苹果渣含有多酚和三萜类化合物等功能性成分,对人体健康有多种益处,但缺乏对其皮肤美容效果的研究。因此,我们在本文中研究了苹果渣提取物(APE)对人体皮肤成纤维细胞(HSFs)的体外影响。用萃取物培养成纤维细胞 72 小时后,浓度为 10 和 20 µg/mL 的成纤维细胞数量有所增加。转录组分析和反转录定量 PCR 结果显示,提取物能上调 HSF 中透明质酸合成酶(HAS)1、HAS2 和 HAS3 的表达,并下调 HYAL1(一种编码透明质酸降解酶的基因)的表达。此外,酶联免疫吸附试验显示,培养上清液中与皮肤细胞外基质有关的因子,如 I 型胶原和透明质酸的分泌量增加。Western印迹结果表明,该提取物可诱导 HSFs 中的细胞外信号调节激酶和蛋白激酶 B 磷酸化。此外,在基因本体分析中还发现了几个与有丝分裂相关的GO_Terms。这是首次研究表明 APE 能诱导 HSFs 增殖并产生与皮肤抗衰老相关的因子。
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来源期刊
Plant Foods for Human Nutrition
Plant Foods for Human Nutrition 工程技术-食品科技
CiteScore
6.80
自引率
7.50%
发文量
89
审稿时长
12-24 weeks
期刊介绍: Plant Foods for Human Nutrition (previously Qualitas Plantarum) is an international journal that publishes reports of original research and critical reviews concerned with the improvement and evaluation of the nutritional quality of plant foods for humans, as they are influenced by: - Biotechnology (all fields, including molecular biology and genetic engineering) - Food science and technology - Functional, nutraceutical or pharma foods - Other nutrients and non-nutrients inherent in plant foods
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