The transcription factor EjNAC5 regulates loquat fruit chilling lignification.

IF 5.6 2区 生物学 Q1 PLANT SCIENCES Journal of Experimental Botany Pub Date : 2024-10-30 DOI:10.1093/jxb/erae333
Yiqing Huang, Zihao Liang, Jiao Lu, Mengxue Zhang, Xizhi Cao, Ruoqian Hu, Dongdong Li, Donald Grierson, Wenbo Chen, Changqing Zhu, Di Wu, Yanna Shi, Kunsong Chen
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Abstract

Changes in both lignin biosynthesis and DNA methylation have been reported to be associated with chilling stress in plants. When stored at low temperatures, red-fleshed loquat is prone to lignification, with increased lignin content and fruit firmness, which has deleterious effects on taste and eating quality. Here, we found that 5 °C storage mitigated the increasing firmness and lignin content of red-fleshed 'Dahongpao' ('DHP') loquat fruit that occurred during 0 °C storage. EjNAC5 was identified by integrating RNA sequencing with whole-genome bisulfite sequencing analysis of 'DHP' loquat fruit. The transcript levels of EjNAC5 were positively correlated with changes in firmness and negatively correlated with changes in DNA methylation level of a differentially methylated region in the EjNAC5 promoter. In white-fleshed 'Baisha' ('BS') loquat fruit, which do not undergo chilling-induced lignification at 0 °C, the transcripts of EjNAC5 remained low and the methylation level of the differentially methylated region in the EjNAC5 promoter was higher, compared with 'DHP' loquat fruit. Transient overexpression of EjNAC5 in loquat fruit and stable overexpression in Arabidopsis and liverwort led to an increase in lignin content. Furthermore, EjNAC5 interacts with EjERF39 and EjHB1 and activates the transcription of Ej4CL1 and EjPRX12 genes involved in lignin biosynthesis. This regulatory network involves different transcription factors from those involved in the lignification pathway. Our study indicates that EjNAC5 promoter methylation modulates EjNAC5 transcript levels and identifies novel EjNAC5-EjERF39-Ej4CL1 and EjNAC5-EjHB1-EjPRX12 regulatory modules involved in chilling induced-lignification.

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EjNAC5 的甲基化及其与其他转录因子的相互作用调控枇杷果实的冷木质化。
据报道,植物木质素生物合成和 DNA 甲基化的变化与寒冷胁迫有关。低温贮藏时,红肉枇杷易发生木质化,木质素含量和果实硬度增加,对口感和食用品质产生有害影响。在这里,我们发现 5°C 贮藏可以缓解红肉枇杷 "大红袍"('DHP')果实在 0°C 贮藏期间出现的坚硬度和木质素含量增加的问题。通过对'DHP'枇杷果实进行RNA测序和全基因组亚硫酸氢盐测序分析,确定了EjNAC5。EjNAC5的转录水平与果实硬度的变化呈正相关,与EjNAC5启动子中不同甲基化区(DMR)的DNA甲基化水平的变化呈负相关。与'DHP'枇杷果实相比,白肉'白沙'('BS')枇杷果实中的EjNAC5转录物仍然较低,而EjNAC5启动子中DMR的甲基化水平较高。在枇杷果实中瞬时过表达 EjNAC5 以及在拟南芥和肝草中稳定过表达 EjNAC5 会导致木质素含量增加。此外,EjNAC5 与 EjERF39 和 EjHB1 相互作用,激活了参与木质素生物合成的 Ej4CL1 和 EjPRX12 基因的转录。这一调控网络涉及与木质素化途径相关的不同 TFs。我们的研究表明,EjNAC5启动子甲基化可调节EjNAC5转录水平,并发现了参与冷害诱导木质化的新型EjNAC5-EjERF39-Ej4CL1和EjNAC5-EjHB1-EjPRX12调控模块。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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