Differences of endophytic microbial compositions and metabolites in roots between fusarium wilt resistant and susceptible melon varieties

IF 5.2 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Chemical and Biological Technologies in Agriculture Pub Date : 2024-07-31 DOI:10.1186/s40538-024-00623-8

Yu Zhu, Yan Yin, Yufei Wei, Jiao-ming Li, Xun Wei, Guifen Li, Yunfeng Ye, Jinyan Huang, Shangdong Yang

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Abstract

Background

Studies have shown that plant endophytic microbial communities are ubiquitous and closely related to plant growth and health. To clarify the mechanism of the melon varieties with high resistant to wilt, the endophytic microbial compositions and metabolites in roots of melon varieties with high resistant ability to wilt were analyzed.

Results

The results showed that the abundances of Firmicutes, Ascomycota, Bacillus, Bradyrhizobium, Amycolatopsis, Actinospica, and Catenulispora all increased in roots of wilt high resistant melon varieties (MT) which compared to wilt susceptible melon varieties (MS). Meanwhile, Ochrobactrum, Bordetella, Roseateles, Staphylococcus, Acidovorax, Amycolatopsis, Catenulispora, Promicromonospora, and Gymnopilus were the unique endophytic microbes in roots of MT. Moreover, in comparison with the MS varieties, the functions of Defense mechanisms, Secondary metabolites biosynthesis, transport and catabolism, Nucleotide transport and metabolism, Signal transduction mechanisms, Coenzyme transport and metabolism, Carbohydrate transport and metabolism and Amino acid transport and metabolism all increased in roots of MT varieties. Additionally, the nucleotide metabolism and biosynthesis of cofactors metabolic pathways were also significantly increased in roots of MT varieties. On the other hand, the untargeted metabolome results showed that Biosynthesis of various plant secondary metabolites, Nucleotide metabolism and Biosynthesis of cofactors metabolic pathways were significantly increased in the expression of MT varieties; and the content of metabolic compounds such as flavonoids, Cinnamic acid compounds, Organic acid compounds, and Nucleotides were increased. In addition, the correlation between microbiome and metabolome indicates a significant correlation between the two.

Conclusions

All above results suggested that higher abundant antagonistic microbes and metabolic functions of endophytes in roots of wilt high resistant melon varieties (MT) were the important mechanisms for their high resistance to wilt.

Graphical Abstract

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抗镰刀菌枯萎病甜瓜品种和易感甜瓜品种根部内生微生物组成和代谢物的差异

研究表明，植物内生微生物群落无处不在，与植物的生长和健康密切相关。为阐明高抗枯萎病甜瓜品种的抗枯萎病机理，对高抗枯萎病甜瓜品种根系内生微生物组成及代谢产物进行了分析。结果表明，与易受枯萎病影响的甜瓜品种（MS）相比，高抗枯萎病甜瓜品种（MT）根系中的固着菌、子囊菌、芽孢杆菌、根瘤菌、淀粉菌、放线菌和Catenulispora的丰度均有所增加。同时，在 MT 的根中，Ochrobactrum、Bordetella、Roseateles、Staphylococcus、Acidovorax、Amycolatopsis、Catenulispora、Promicromonospora 和 Gymnopilus 是特有的内生微生物。此外，与 MS 品种相比，MT 品种根系中的防御机制、次生代谢产物的生物合成、运输和分解、核苷酸运输和代谢、信号转导机制、辅酶运输和代谢、碳水化合物运输和代谢以及氨基酸运输和代谢的功能都有所增加。此外，核苷酸代谢和辅因子生物合成代谢途径在 MT 品种的根中也显著增加。另一方面，非靶向代谢组结果显示，在 MT 品种的表达中，各种植物次生代谢物的生物合成、核苷酸代谢和辅助因子的生物合成代谢途径明显增加；代谢化合物如黄酮类化合物、肉桂酸化合物、有机酸化合物和核苷酸的含量也有所增加。此外，微生物组和代谢组之间的相关性表明二者之间存在显著的相关性。上述结果表明，高抗枯萎病甜瓜品种（MT）根系中较多的拮抗微生物和内生菌的代谢功能是其高抗枯萎病的重要机制。

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来源期刊

Chemical and Biological Technologies in Agriculture Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

6.80

自引率

3.00%

发文量

审稿时长

15 weeks

期刊介绍： Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture. This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population. Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.