Genome-wide identification of the AP2/ERF gene family from Limonium bicolor and functional characterization of LbAP2/ERF32 under salt stress

IF 6.1 2区生物学 Q1 PLANT SCIENCES Plant Physiology and Biochemistry Pub Date : 2024-08-09 DOI:10.1016/j.plaphy.2024.109035

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Abstract

AP2/ERF transcription factors (TFs) play important roles in plant growth and development, plant morphogenesis and response to environmental stresses. However, their biological roles in recretohalophytes are still not fully revealed. Limonium bicolor L. is a typical recretohalophyte, which secretes excessive salt ions through the salt glands on the epidermis. Here, 64 LbAP2/ERF genes were identified in L. bicolor genome, which were unevenly distributed on the eight chromosomes. Cis-elements related to growth and development, stress response and phytohormone response are distributed in multiple LbAP2/ERF promoters. Expression analysis indicated that LbAP2/ERF genes responsed to NaCl, PEG and ABA. And the salt gland density, salt secretion of leaves and overall salt tolerance of LbAP2/ERF32 silenced lines were significantly reduced. In agreement, the genes related to salt gland development and ion transport were significantly changed in LbAP2/ERF32-silenced lines. Our findings provided fundamental information on the structure and evolutionary relationship of LbAP2/ERF gene family in salt gland development and salt secretion of L. bicolor and gave theoretical guideline for further functional study of LbAP2/ERF genes in response to abiotic stress.

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盐胁迫下 Limonium bicolor 的 AP2/ERF 基因家族的全基因组鉴定和 LbAP2/ERF32 的功能特征。

AP2/ERF 转录因子（TFs）在植物生长发育、植物形态发生和对环境胁迫的响应中发挥着重要作用。然而，它们在退叶性植物中的生物学作用仍未完全揭示。Limonium bicolor L.是一种典型的返盐叶绿藻，它通过表皮上的盐腺分泌过量的盐离子。本研究在 L. bicolor 基因组中发现了 64 个 LbAP2/ERF 基因，这些基因不均匀地分布在 8 条染色体上。与生长发育、胁迫反应和植物激素反应有关的顺式元件分布在多个 LbAP2/ERF 启动子中。表达分析表明，LbAP2/ERF 基因对 NaCl、PEG 和 ABA 有响应。LbAP2/ERF32沉默株的盐腺密度、叶片盐分分泌量和整体耐盐性均显著降低。同样，与盐腺发育和离子转运相关的基因在LbAP2/ERF32沉默株中也发生了显著变化。我们的研究结果为LbAP2/ERF基因家族在盐腺发育和盐分分泌中的结构和进化关系提供了基础信息，为进一步研究LbAP2/ERF基因在非生物胁迫下的功能提供了理论指导。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.