Tolerance Mechanisms and Removal Efficiency of Chlorella pyrenoidosa in Treating 3-Fluorophenol Pollution.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Metabolites Pub Date : 2024-08-15 DOI:10.3390/metabo14080449

Min Li, Zhenfang Shang, Yonglan Ma, Huijun Zhao, Zhijing Ni, Zhaojun Wei, Xiu Zhang

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Abstract

This study investigates the growth tolerance mechanisms of Chlorella pyrenoidosa to 3-fluorophenol and its removal efficiency by algal cells. Our results indicate that C. pyrenoidosa can tolerate up to 100 mg/L of 3-fluorophenol, exhibiting a significant hormesis effect characterized by initial inhibition followed by promotion of growth. In C. pyrenoidosa cells, the activities of superoxide dismutase (SOD) and catalase (CAT), as well as the levels of malondialdehyde (MDA) and reactive oxygen species (ROS), were higher than or comparable to the control group. Metabolic analysis revealed that the 3-fluorophenol treatment activated pathways, such as glycerol phospholipid metabolism, autophagy, glycosylphosphatidylinositol (GPI)-anchored protein biosynthesis, and phenylpropanoid biosynthesis, contributed to the stabilization of cell membrane structures and enhanced cell repair capacity. After 240 h of treatment, over 50% of 3-fluorophenol was removed by algal cells, primarily through adsorption. Thus, C. pyrenoidosa shows potential as an effective biosorbent for the bioremediation of 3-fluorophenol.

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小球藻在处理 3-氟苯酚污染中的耐受机制和去除效率

本研究探讨了拟小球藻对 3-氟苯酚的生长耐受机制以及藻细胞对 3-氟苯酚的去除效率。结果表明，焦绿球藻能耐受高达 100 mg/L 的 3-氟苯酚，并表现出显著的激素效应，其特点是先抑制生长，然后促进生长。在类焦藻类细胞中，超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性以及丙二醛（MDA）和活性氧（ROS）的水平均高于或与对照组相当。代谢分析表明，3-氟苯酚处理激活了甘油磷脂代谢、自噬、糖基磷脂酰肌醇（GPI）锚定蛋白生物合成和苯丙醇类生物合成等途径，有助于稳定细胞膜结构和增强细胞修复能力。经过 240 小时的处理后，超过 50% 的 3-氟苯酚被藻类细胞去除，主要是通过吸附作用。因此，C. pyrenoidosa 有可能成为一种有效的生物吸附剂，用于 3-氟苯酚的生物修复。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.