Comprehensive Secondary Metabolite Profiling and Antioxidant Activity of Aqueous and Ethanol Extracts of Neolamarckia cadamba (Roxb.) Bosser Fruits.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Metabolites Pub Date : 2024-09-21 DOI:10.3390/metabo14090511

Lin Yang, Liyan Wu, Yongxin Li, Yuhui Yang, Yuting Gu, Jialin Yang, Luzy Zhang, Fanxin Meng

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Abstract

Background: Neolamarckia cadamba (Rubiaceae) is a well-recognized medicinal plant with recorded therapeutical attributes. However, a thorough assessment of active compounds in its fruits is lacking, limiting their use and valorization in pharmacological industries.

Methods: Thus, this study investigated variations in the fruits' secondary metabolite (SM) profiles, as well as antioxidant activities in aqueous (WA) and ethanol (ET) extracts.

Results: Liquid chromatography-electrospray ionization tandem mass spectrometry identified 541 SMs, of which 14 and 1 (di-O-glucosylquinic acid) were specifically detected in ET and WA, respectively. Phenolic acids (36.97%), flavonoids (28.10%), terpenoids (12.20%), and alkaloids (9.98%) were the dominant SMs. The SM profiles of the fruits in WA and ET were quite different. We revealed 198 differentially extracted (DE) metabolites between WA and ET, including 62 flavonoids, 57 phenolic acids, 45 terpenoids, 14 alkaloids, etc. Most DE flavones (36 out of 40), terpenoids (45 out of 45), and alkaloids (12 out of 14) had higher content in ET. Catechin and its derivatives, procyanidins, and tannins had higher content in WA. ABTS and DPPH assays showed that the antioxidant activity of ET was significantly higher than that of WA.

Conclusions: Our findings will facilitate the efficient extraction and evaluation of specific active compounds in N. cadamba.

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Neolamarckia cadamba (Roxb.) Bosser 果实水提取物和乙醇提取物的全面次生代谢物分析和抗氧化活性。

背景：茜草科（Rubiaceae）茜草属（Neolamarckia cadamba）是一种广受认可的药用植物，其治疗特性已被记录在案。然而，由于缺乏对其果实中活性化合物的全面评估，限制了其在制药业中的应用和价值提升：因此，本研究调查了果实中次生代谢物（SM）的变化，以及水提取物（WA）和乙醇提取物（ET）中的抗氧化活性：液相色谱-电喷雾串联质谱法鉴定出 541 种次生代谢物，其中 14 种（二-O-葡萄糖基奎宁酸）和 1 种（二-O-葡萄糖基奎宁酸）分别在 ET 和 WA 中被特别检测到。酚酸类（36.97%）、黄酮类（28.10%）、萜类（12.20%）和生物碱类（9.98%）是主要的 SMs。西澳和东澳果实的 SM 图谱差异很大。我们发现西澳和东澳的差异提取代谢物有 198 种，包括 62 种黄酮类化合物、57 种酚酸类化合物、45 种萜类化合物和 14 种生物碱类化合物等。大多数 DE 黄酮类化合物（40 种中的 36 种）、萜类化合物（45 种中的 45 种）和生物碱（14 种中的 12 种）在 ET 中含量较高。儿茶素及其衍生物、原花青素和单宁酸在 WA 中含量较高。ABTS 和 DPPH 试验表明，ET 的抗氧化活性明显高于 WA：我们的研究结果将有助于有效提取和评估 N. cadamba 中的特定活性化合物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.