Identification of potential pathogenic genes related to osteoporosis and osteoarthritis.

IF 1.4 4区医学 Q4 ENGINEERING, BIOMEDICAL Technology and Health Care Pub Date : 2024-07-16 DOI:10.3233/THC-240574

Zhanchao Wang, Wei Wang, Bin Zuo, Hua Lu

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引用次数: 0

Abstract

Background: Osteoarthritis (OA) and osteoporosis (OS) are the most common orthopedic diseases.

Objective: To identify important genes as biomarkers for the pathogenesis of OA and OS.

Methods: Microarray data for OA and OS were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) between the OA and healthy control groups and between the OS and healthy control groups were identified using the Limma software package. Overlapping hub DEGs were selected using MCC, MNC, DEGREE, and EPC. Weighted gene co-expression network analysis (WGCNA) was used to mine OA- and OS-related modules. Shared hub DEGs were identified, human microRNA disease database was used to screen microRNAs associated with OA and OS, and an miRNA-target gene network was constructed. Finally, the expression of shared hub DEGs was evaluated.

Results: A total of 104 overlapping DEGs were identified in both the OA and OS groups, which were mainly related to inflammatory biological processes, such as the Akt and TNF signaling pathways Forty-six hub DEGs were identified using MCC, MNC, DEGREE, and EPC modules using different algorithms. Seven modules with 392 genes that highly correlated with disease were identified in the WGCNA. Furthermore, 10 shared hub DEGs were identified between the OA and OS groups, including OGN, FAP, COL6A3, THBS4, IGFBP2, LRRC15, DDR2, RND3, EFNB2, and CD48. A network consisting of 8 shared hub DEGs and 55 miRNAs was constructed. Furthermore, CD48 was significantly upregulated in the OA and OS groups, whereas EFNB2, DR2, COL6A3, and RND3 were significantly downregulated in OA and OS. Other hub DEGs were significantly upregulated in OA and downregulated in OS.

Conclusions: The ten genes may be promising biomarkers for modulating the development of both OA and OS.

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鉴定与骨质疏松症和骨关节炎有关的潜在致病基因。

背景：骨关节炎和骨质疏松症是最常见的骨科疾病：骨关节炎（OA）和骨质疏松症（OS）是最常见的骨科疾病：方法：从基因表达 Oray 中下载 OA 和 OS 的芯片数据：方法：从基因表达总库（Gene Expression Omnibus）数据库下载 OA 和 OS 的芯片数据。使用Limma软件包鉴定OA组和健康对照组之间以及OS组和健康对照组之间的差异表达基因（DEGs）。使用 MCC、MNC、DEGREE 和 EPC 筛选出重叠的中枢 DEGs。加权基因共表达网络分析（WGCNA）用于挖掘OA和OS相关模块。确定了共享的中枢DEG，利用人类microRNA疾病数据库筛选了与OA和OS相关的microRNA，并构建了miRNA-靶基因网络。最后，对共享中枢 DEGs 的表达进行了评估：结果：在 OA 组和 OS 组共发现了 104 个重叠的 DEGs，这些 DEGs 主要与炎症生物过程有关，如 Akt 和 TNF 信号转导通路使用 MCC、MNC、DEGREE 和 EPC 模块，通过不同的算法识别出 46 个中枢 DEGs。在 WGCNA 中发现了 7 个模块，包含 392 个与疾病高度相关的基因。此外，OA组和OS组之间还发现了10个共享的中枢DEG，包括OGN、FAP、COL6A3、THBS4、IGFBP2、LRRC15、DDR2、RND3、EFNB2和CD48。由 8 个共享的中枢 DEGs 和 55 个 miRNAs 构建了一个网络。此外，CD48在OA组和OS组中明显上调，而EFNB2、DR2、COL6A3和RND3在OA组和OS组中明显下调。其他枢纽DEG在OA中明显上调，在OS中明显下调：结论：这十个基因可能是调节 OA 和 OS 发展的有希望的生物标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Technology and Health Care HEALTH CARE SCIENCES & SERVICES-ENGINEERING, BIOMEDICAL

CiteScore

2.10

自引率

6.20%

发文量

282

审稿时长

>12 weeks

期刊介绍： Technology and Health Care is intended to serve as a forum for the presentation of original articles and technical notes, observing rigorous scientific standards. Furthermore, upon invitation, reviews, tutorials, discussion papers and minisymposia are featured. The main focus of THC is related to the overlapping areas of engineering and medicine. The following types of contributions are considered: 1.Original articles: New concepts, procedures and devices associated with the use of technology in medical research and clinical practice are presented to a readership with a widespread background in engineering and/or medicine. In particular, the clinical benefit deriving from the application of engineering methods and devices in clinical medicine should be demonstrated. Typically, full length original contributions have a length of 4000 words, thereby taking duly into account figures and tables. 2.Technical Notes and Short Communications: Technical Notes relate to novel technical developments with relevance for clinical medicine. In Short Communications, clinical applications are shortly described. 3.Both Technical Notes and Short Communications typically have a length of 1500 words. Reviews and Tutorials (upon invitation only): Tutorial and educational articles for persons with a primarily medical background on principles of engineering with particular significance for biomedical applications and vice versa are presented. The Editorial Board is responsible for the selection of topics. 4.Minisymposia (upon invitation only): Under the leadership of a Special Editor, controversial or important issues relating to health care are highlighted and discussed by various authors. 5.Letters to the Editors: Discussions or short statements (not indexed).