RNA helicase Brr2a promotes miRNA biogenesis by properly remodelling secondary structure of pri-miRNAs

IF 15.8 1区生物学 Q1 PLANT SCIENCES Nature Plants Pub Date : 2024-09-13 DOI:10.1038/s41477-024-01788-8

Xindi Li, Songxiao Zhong, Changhao Li, Xingxing Yan, Jiaying Zhu, Yanjun Li, Zhiye Wang, Xu Peng, Xiuren Zhang

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Abstract

RNA secondary structure (RSS) of primary microRNAs (pri-miRNAs) is a key determinant for miRNA production. Here we report that RNA helicase (RH) Brr2a, best known as a spliceosome component, modulates the structural complexity of pri-miRNAs to fine tune miRNA yield. Brr2a interacts with microprocessor component HYL1 and its loss reduces the levels of miRNAs derived from both intron-containing and intron-lacking pri-miRNAs. Brr2a binds to pri-miRNAs in vivo and in vitro. Furthermore, Brr2a hydrolyses ATP and the activity can be significantly enhanced by pri-miRNAs. Consequently, Brr2a unwinds pri-miRNAs in vitro. Moreover, Brr2a variants with compromised ATPase or RH activity are incapable of unwinding pri-miRNA, and their transgenic plants fail to restore miRNA levels in brr2a-2. Importantly, most of tested pri-miRNAs display distinct RSS, rendering them unsuitable for efficient processing in brr2a mutants vs Col-0. Collectively, this study reveals that Brr2a plays a non-canonical role in miRNA production beyond splicing regulation.

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RNA 螺旋酶 Brr2a 通过适当重塑 pri-miRNA 的二级结构促进 miRNA 的生物发生

初级微RNA（pri-miRNA）的RNA二级结构（RSS）是决定miRNA产量的关键因素。在这里，我们报告了 RNA 螺旋酶（RH）Brr2a--众所周知的剪接体成分--调节 pri-miRNA 的结构复杂性，以微调 miRNA 产量。Brr2a与微处理器元件HYL1相互作用，失去HYL1会降低由含内含子和缺内含子的pri-miRNA产生的miRNA水平。Brr2a 可在体内和体外与 pri-miRNA 结合。此外，Brr2a 能水解 ATP，而 pri-miRNA 能显著增强其活性。因此，Brr2a 能在体外解开 pri-miRNA。此外，ATP 酶或 RH 活性受损的 Brrr2a 变体无法解开 pri-miRNA，其转基因植株也无法恢复 brrr2a-2 中的 miRNA 水平。重要的是，大多数测试的 pri-miRNA 显示出不同的 RSS，使它们不适合在 brr2a 突变体与 Col-0 中进行有效处理。总之，这项研究揭示了 Brrr2a 在 miRNA 的产生中发挥着剪接调控之外的非规范作用。

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来源期刊

Nature Plants PLANT SCIENCES-

CiteScore

25.30

自引率

2.20%

发文量

196

期刊介绍： Nature Plants is an online-only, monthly journal publishing the best research on plants — from their evolution, development, metabolism and environmental interactions to their societal significance.

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