Immunofluorescent Localization of Ca2+ Sensor Proteins in Somatic Motor Muscles of the Earthworm Lumbricus terrestris

Pub Date : 2024-08-29 DOI:10.1134/s0022093024040045
L. F. Nurullin, N. D. Almazov, E. M. Volkov
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Abstract

Immunofluorescent labeling revealed calmodulin, Ca2+/calmodulin-dependent protein kinases I and II, synaptotagmin II and VII, and calcineurin A in somatic motor muscles of the earthworm Lumbricus terrestris. These Ca2+ sensor proteins are detected in both synaptic and extrasynaptic regions. However, synaptotagmin II and VII, as well as calcineurin A, are mainly localized in the area of neuromuscular junctions, with the synaptic localization for synaptotagmin VII and calcineurin A being pronounced most distinctly.

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蚯蚓躯体运动肌肉中 Ca2+ 传感器蛋白的免疫荧光定位
摘要免疫荧光标记显示,钙调蛋白、Ca2+/钙调蛋白依赖蛋白激酶Ⅰ和Ⅱ、突触位点蛋白Ⅱ和Ⅶ以及钙调素A存在于蚯蚓的躯体运动肌肉中。然而,突触位点蛋白 II 和 VII 以及钙调素 A 主要定位在神经肌肉接头区域,其中突触位点蛋白 VII 和钙调素 A 的突触定位最为明显。
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