Investigations into the Protein Stabilization of Musts and Wines by Aspergillopepsin under Different Enzymatic and Thermal Treatments

IF 2.6 Q2 FOOD SCIENCE & TECHNOLOGY ACS food science & technology Pub Date : 2024-08-15 DOI:10.1021/acsfoodscitech.4c00308
Leif Seidel, Katrin Runkel, Wendell Albuquerque, Katharina Happel, Parviz Ghezellou, Bernhard Spengler, Holger Zorn, Martin Gand, Maximilian Freund, Frank Will, Ralf Schweiggert
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Abstract

Protein removal with aspergillopepsin in combination with short-time heating has recently emerged as an opportunity for winemakers to prevent protein haze in wine. In this study, we first treated a protein-rich must and wine with two different aspergillopepsin preparations along an identical control treatment without enzyme addition on a pilot plant scale. While treating wines was rather ineffective, treating musts was effective in reducing protein concentrations by up to 86%, leading to haze-stable wines according to the common heat test. Varying thermal loads in further experiments, a rather mild heat treatment of 65 °C for 30 s compared to those in earlier studies was found sufficient for enzymatic protein degradation. Treating must from a fungus-resistant variety (cv. Fidelio) was as successful as treating must from a blend of conventional varieties (cv. Gewürztraminer/Müller-Thurgau). Triangle sensory tests found no clear evidence of an effect on the quality by heat treatments with and without enzyme dosage.

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关于曲霉胃蛋白酶在不同酶解和热处理条件下稳定葡萄汁和葡萄酒蛋白质的研究
最近,使用曲霉胃蛋白酶去除蛋白质并结合短时间加热成为酿酒师防止葡萄酒中蛋白质混浊的一个机会。在这项研究中,我们首先用两种不同的曲霉胃蛋白酶制剂处理富含蛋白质的葡萄汁和葡萄酒,同时在试验工厂规模上进行不添加酶的相同对照处理。虽然对葡萄酒的处理效果不佳,但对葡萄汁的处理却能有效降低蛋白质浓度,降幅高达 86%,从而使葡萄酒在普通的热试验中具有稳定的雾度。在进一步的实验中,由于热负荷的不同,与之前的研究相比,65 °C 30 秒的温和热处理足以酶解蛋白质。处理抗真菌品种(Fidelio)的葡萄汁与处理传统品种(Gewürztraminer/Müller-Thurgau)混合的葡萄汁一样成功。三角感官测试没有发现明显的证据表明加酶和不加酶的热处理会影响葡萄汁的质量。
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