Sesha Subramanian Murugan, Pandurang Appana Dalavi, Pramod K Gupta, Won Hur, Ramkumar Pillappan, Jayachandran Venkatesan and Gi Hun Seong
{"title":"Saponin-mediated and microwave-assisted biosynthesis of silver nanoparticles: preparations and anticancer assessment","authors":"Sesha Subramanian Murugan, Pandurang Appana Dalavi, Pramod K Gupta, Won Hur, Ramkumar Pillappan, Jayachandran Venkatesan and Gi Hun Seong","doi":"10.1088/2043-6262/ad71a9","DOIUrl":null,"url":null,"abstract":"Silver nanoparticles have been extensively studied and used for biological and biomedical applications due to its antimicrobial properties. In this study, we have developed silver nanoparticles with saponin (S-AgNPs) using microwave-mediated methodology and physiochemically characterised and evaluated its anticancer potential. According to UV–visible spectroscopy, S-AgNPs exhibited a maximum absorption peak at a wavelength of 420 nm. DLS analysis revealed that S-AgNPs have an average diameter of around 133.8 ± 10 nm. HR-TEM analysis confirms the spherical morphology of S-AgNPs. Further, the anticancer effect of S-AgNPs was validated using A459 lung cancer cells by in vitro cell interaction tests such as the MTT assay, staining assay, and flow cytometry assay. The IC50 value of S-AgNPs against A549 cells was 30 μg ml−1, and Calcein-EtBr and Annexin V/PI staining results confirm the presence of apoptotic cells after treatment S-AgNPs. Hence, biosynthesized S-AgNPs can play a vital role in developing anticancer drugs for cancer treatment.","PeriodicalId":7359,"journal":{"name":"Advances in Natural Sciences: Nanoscience and Nanotechnology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Natural Sciences: Nanoscience and Nanotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1088/2043-6262/ad71a9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
Silver nanoparticles have been extensively studied and used for biological and biomedical applications due to its antimicrobial properties. In this study, we have developed silver nanoparticles with saponin (S-AgNPs) using microwave-mediated methodology and physiochemically characterised and evaluated its anticancer potential. According to UV–visible spectroscopy, S-AgNPs exhibited a maximum absorption peak at a wavelength of 420 nm. DLS analysis revealed that S-AgNPs have an average diameter of around 133.8 ± 10 nm. HR-TEM analysis confirms the spherical morphology of S-AgNPs. Further, the anticancer effect of S-AgNPs was validated using A459 lung cancer cells by in vitro cell interaction tests such as the MTT assay, staining assay, and flow cytometry assay. The IC50 value of S-AgNPs against A549 cells was 30 μg ml−1, and Calcein-EtBr and Annexin V/PI staining results confirm the presence of apoptotic cells after treatment S-AgNPs. Hence, biosynthesized S-AgNPs can play a vital role in developing anticancer drugs for cancer treatment.