Saponin-mediated and microwave-assisted biosynthesis of silver nanoparticles: preparations and anticancer assessment

IF 1.7 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Advances in Natural Sciences: Nanoscience and Nanotechnology Pub Date : 2024-09-10 DOI:10.1088/2043-6262/ad71a9

Sesha Subramanian Murugan, Pandurang Appana Dalavi, Pramod K Gupta, Won Hur, Ramkumar Pillappan, Jayachandran Venkatesan and Gi Hun Seong

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Abstract

Silver nanoparticles have been extensively studied and used for biological and biomedical applications due to its antimicrobial properties. In this study, we have developed silver nanoparticles with saponin (S-AgNPs) using microwave-mediated methodology and physiochemically characterised and evaluated its anticancer potential. According to UV–visible spectroscopy, S-AgNPs exhibited a maximum absorption peak at a wavelength of 420 nm. DLS analysis revealed that S-AgNPs have an average diameter of around 133.8 ± 10 nm. HR-TEM analysis confirms the spherical morphology of S-AgNPs. Further, the anticancer effect of S-AgNPs was validated using A459 lung cancer cells by in vitro cell interaction tests such as the MTT assay, staining assay, and flow cytometry assay. The IC50 value of S-AgNPs against A549 cells was 30 μg ml−1, and Calcein-EtBr and Annexin V/PI staining results confirm the presence of apoptotic cells after treatment S-AgNPs. Hence, biosynthesized S-AgNPs can play a vital role in developing anticancer drugs for cancer treatment.

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皂素介导和微波辅助的银纳米粒子生物合成：制备和抗癌评估

银纳米粒子因其抗菌特性而被广泛研究并用于生物和生物医学应用。在这项研究中，我们采用微波介导的方法开发了含皂苷（S-AgNPs）的银纳米粒子，并对其进行了生化表征和抗癌潜力评估。紫外可见光谱显示，S-AgNPs 在 420 纳米波长处有最大吸收峰。DLS 分析显示，S-AgNPs 的平均直径约为 133.8 ± 10 nm。HR-TEM 分析证实了 S-AgNPs 的球形形态。此外，还利用 A459 肺癌细胞，通过 MTT 试验、染色试验和流式细胞术试验等体外细胞相互作用试验验证了 S-AgNPs 的抗癌效果。S-AgNPs 对 A549 细胞的 IC50 值为 30 μg ml-1，而 Calcein-EtBr 和 Annexin V/PI 染色结果证实了 S-AgNPs 处理后细胞凋亡的存在。因此，生物合成的 S-AgNPs 可在开发抗癌药物治疗癌症方面发挥重要作用。

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Advances in Natural Sciences: Nanoscience and Nanotechnology NANOSCIENCE & NANOTECHNOLOGYMATERIALS SCIE-MATERIALS SCIENCE, MULTIDISCIPLINARY

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