Antimicrobial activity of apricot kernel extract loaded carboxymethyl chitosan nanoparticles against multidrug resistant wound-skin infection bacteria

IF 1.7 Q3 MATERIALS SCIENCE, MULTIDISCIPLINARY Advances in Natural Sciences: Nanoscience and Nanotechnology Pub Date : 2024-08-16 DOI:10.1088/2043-6262/ad6c0b
A S El-Houssiny, E A Fouad
{"title":"Antimicrobial activity of apricot kernel extract loaded carboxymethyl chitosan nanoparticles against multidrug resistant wound-skin infection bacteria","authors":"A S El-Houssiny, E A Fouad","doi":"10.1088/2043-6262/ad6c0b","DOIUrl":null,"url":null,"abstract":"In recent years, skin and soft-tissue infections, particularly due to multidrug resistance bacteria (MDR) are generating a serious health crisis to human health. Thus, the current investigation tried to find new promising alternatives such as herbal therapy and biopolymer nanotechnology to combat MDR microbes. Apricot kernels extract was prepared and its amygdalin content was determined by HPLC analysis. Carboxymethyl chitosan nanoparticles (CMC NPs) encapsulated with amygdalin extract (Am ext) were synthesized and characterized through their morphology, particle size, zeta potential and thermal analysis. The antibacterial activity of Am ext, CMC NPs and CMC-Am ext NPs were evaluated against MDR bacteria. Moreover, to confirm the antibacterial action of the samples, bacterial DNA fragmentation analysis was performed. Furthermore, the cyanide ions released from bacterial breakdown of amygdalin was confirmed using Nanocolor Cyanide 08 Test 0–31 kits. The HPLC analysis indicated that amygdalin extracted efficiently from the apricot kernels. The CMC-Am ext NPs exhibited spherical shaped and mono dispersed particles of size 28 nm; physical stability and thermal compatibility. Additionally, CMC-Am ext NPs have significant antibacterial action on all MDR microbes in synergy with Am ext. Moreover, the results confirmed that the cyanide ions were released from amygdalin breakdown by the action of bacteria. Furthermore, the DNA fragmentation analysis confirmed that both Am ext and its nano-encapsulated form caused bacterial cell death by inducing DNA damage. Therefore, these findings demonstrate CMC-Am ext NPs as a novel potential therapeutic agent which can be used as an alternative to the current antibiotics against MDR bacteria.","PeriodicalId":7359,"journal":{"name":"Advances in Natural Sciences: Nanoscience and Nanotechnology","volume":null,"pages":null},"PeriodicalIF":1.7000,"publicationDate":"2024-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advances in Natural Sciences: Nanoscience and Nanotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1088/2043-6262/ad6c0b","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0

Abstract

In recent years, skin and soft-tissue infections, particularly due to multidrug resistance bacteria (MDR) are generating a serious health crisis to human health. Thus, the current investigation tried to find new promising alternatives such as herbal therapy and biopolymer nanotechnology to combat MDR microbes. Apricot kernels extract was prepared and its amygdalin content was determined by HPLC analysis. Carboxymethyl chitosan nanoparticles (CMC NPs) encapsulated with amygdalin extract (Am ext) were synthesized and characterized through their morphology, particle size, zeta potential and thermal analysis. The antibacterial activity of Am ext, CMC NPs and CMC-Am ext NPs were evaluated against MDR bacteria. Moreover, to confirm the antibacterial action of the samples, bacterial DNA fragmentation analysis was performed. Furthermore, the cyanide ions released from bacterial breakdown of amygdalin was confirmed using Nanocolor Cyanide 08 Test 0–31 kits. The HPLC analysis indicated that amygdalin extracted efficiently from the apricot kernels. The CMC-Am ext NPs exhibited spherical shaped and mono dispersed particles of size 28 nm; physical stability and thermal compatibility. Additionally, CMC-Am ext NPs have significant antibacterial action on all MDR microbes in synergy with Am ext. Moreover, the results confirmed that the cyanide ions were released from amygdalin breakdown by the action of bacteria. Furthermore, the DNA fragmentation analysis confirmed that both Am ext and its nano-encapsulated form caused bacterial cell death by inducing DNA damage. Therefore, these findings demonstrate CMC-Am ext NPs as a novel potential therapeutic agent which can be used as an alternative to the current antibiotics against MDR bacteria.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
杏核提取物负载的羧甲基壳聚糖纳米颗粒对耐多药伤口皮肤感染细菌的抗菌活性
近年来,皮肤和软组织感染,尤其是由多重耐药菌(MDR)引起的感染,正在给人类健康带来严重的健康危机。因此,目前的研究试图寻找新的有前途的替代方法,如草药疗法和生物聚合物纳米技术来对抗 MDR 微生物。研究人员制备了杏核提取物,并通过高效液相色谱分析测定了其中的杏仁苷含量。合成了包裹杏仁苷提取物(Am ext)的羧甲基壳聚糖纳米颗粒(CMC NPs),并通过形态、粒度、ZETA电位和热分析对其进行了表征。评估了 Am ext、CMC NPs 和 CMC-Am ext NPs 对 MDR 细菌的抗菌活性。此外,为了证实样品的抗菌作用,还进行了细菌 DNA 片段分析。此外,还使用 Nanocolor Cyanide 08 Test 0-31 试剂盒确认了细菌分解苦杏仁苷释放出的氰离子。高效液相色谱分析表明,杏仁苷能从杏核中有效提取。CMC-Am ext NPs 呈球形,单分散颗粒大小为 28 nm,具有物理稳定性和热相容性。此外,CMC-Am ext NPs 与 Am ext 协同作用,对所有 MDR 微生物都有显著的抗菌作用。此外,DNA 片段分析证实,Am ext 及其纳米包囊形式都通过诱导 DNA 损伤导致细菌细胞死亡。因此,这些研究结果证明了 CMC-Am ext NPs 是一种新型的潜在治疗剂,可作为现有抗生素的替代品来对付 MDR 细菌。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Advances in Natural Sciences: Nanoscience and Nanotechnology
Advances in Natural Sciences: Nanoscience and Nanotechnology NANOSCIENCE & NANOTECHNOLOGYMATERIALS SCIE-MATERIALS SCIENCE, MULTIDISCIPLINARY
自引率
4.80%
发文量
0
期刊最新文献
Recent advancements of nanoparticles for antiviral therapy Saponin-mediated and microwave-assisted biosynthesis of silver nanoparticles: preparations and anticancer assessment Synthesis, characterization, and cellular investigation of three smart polymeric nanoparticles as efficient plasmid CRISPR (pCRISPR) delivery vehicles Chitosan derived N-doped carbon aerogel nanostructures for high-performance supercapacitors Synergistic effect of cobalt ferrite-graphene oxide based hyperthermia and capsaicin to induce apoptosis and inhibit telomerase activity in breast cancer cells
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1