The effect of cold plasma on the treatment of external otitis: an experimental study in rats

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-09-11 DOI:10.1186/s13568-024-01748-7
Tayebe Taghizade, Alireza Akbarzadeh-Baghban, Nasrin Navab Safa
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Abstract

In this paper we investigate the influence of cold plasma as novel method on the external otitis treatment which is a frequent cause of earache. 24 infected external auditory canals in 24 rats were categorized in four experimental groups including control, plasma exposed, ciprofloxacin drug and mixed of plasma-ciprofloxacin groups. In plasma group, dielectric barrier discharge was employed as the source of cold plasma in 5 days. All rats were observed with otoscope daily and a scoring system was used to evaluate swelling and effusion of the ear canal. Number of colonies in microbiological culture were counted in each group during the first 5 days after treatment. For the multiple group comparisons of swelling and effusion measured in the external auditory canal, Kruskal–Wallis analysis was applied and one-way anova and Kruskal–Wallis analysis was used for the statistical analysis of the results of the cultures in different days. Also, Tukey and Mann–Whitney tests was applied for multiple comparisons. Our findings show that swelling and effusion were obviously reduced in plasma group compared to control group (P < 0.01). Number of colonies in control group was statistically different from those in drug, plasma, and mixed group on the second to fifth day (p < 0.001). According to the results cold plasma can be introduced as an impressive method for external otitis treatment. Moreover, when cold plasma joined to antibiotic method, it leads to a superior performance respecting plasma or antibiotic method alone.

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冷血浆治疗外耳道炎的效果:大鼠实验研究
本文研究了冷等离子体作为一种新方法对外耳道炎治疗的影响,外耳道炎是耳痛的常见原因之一。我们将 24 只感染外耳道炎的大鼠分为四个实验组,包括对照组、血浆暴露组、环丙沙星药物组和血浆-环丙沙星混合组。等离子组采用介质阻挡放电作为冷等离子源,为期 5 天。每天用耳镜观察所有大鼠,并使用评分系统评估耳道肿胀和渗出情况。在治疗后的前 5 天,对每组大鼠的微生物培养菌落数进行计数。外耳道肿胀和渗出的多组比较采用 Kruskal-Wallis 分析法,不同天数培养结果的统计分析采用单向 anova 和 Kruskal-Wallis 分析法。此外,还采用 Tukey 和 Mann-Whitney 检验进行多重比较。我们的研究结果表明,与对照组相比,血浆组的肿胀和渗出明显减少(P < 0.01)。对照组与药物组、血浆组和混合组在第二至第五天的菌落数有统计学差异(P <0.001)。结果表明,冷血浆可作为一种令人印象深刻的外耳道炎治疗方法。此外,当冷等离子与抗生素方法结合使用时,其疗效优于单独使用等离子或抗生素的方法。
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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