Seabuckthorn polysaccharides mitigate hepatic steatosis by modulating the Nrf-2/HO-1 pathway and gut microbiota

IF 3.5 3区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY AMB Express Pub Date : 2024-09-09 DOI:10.1186/s13568-024-01756-7
Yan Yan, Haisheng Yuan, Fan Yang, Heiya Na, Xiuling Yu, Jingran Liu, Yuzhen Wang
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Abstract

Non-alcoholic fatty liver disease (NAFLD) is becoming a significant global public health threat. Seabuckthorn (Hippophae rhamnoides L.) has been used in traditional Chinese medicine (TCM). The hypolipidemic effects of Seabuckthorn polysaccharides (SP) against high-fat diets (HFD)-induced NAFLD were systematically explored and compared with that of Bifidobacterium lactis V9 (B. Lactis V9). Results showed that HFD-induced alanine transaminase (ALT) and aspartate aminotransferase (AST) levels decreased by 2.8-fold and 4.5-fold, respectively, after SP supplementation. Moreover, the alleviating effect on hepatic lipid accumulation is better than that of B. Lactis V9. The ACC and FASN mRNA levels were significantly reduced by 1.8 fold (P < 0.05) and 2.3 folds (P < 0.05), respectively, while the CPT1α and PPARα mRNA levels was significantly increased by 2.3 fold (P < 0.05) and 1.6 fold (P < 0.05), respectively, after SP administration. SP activated phosphorylated-AMPK and inhibited PPARγ protein expression, improved serum oxidative stress and inflammation (P < 0.05). SP supplementation leads to increased hepatic expression of nuclear factor erythroid 2-related factor 2 (Nrf-2), heme oxygenase-1 (HO-1) and Superoxide dismutase-2 (SOD-2). Furthermore, SP treatment improved HFD-induced intestinal dysbiosis. Lentisphaerae, Firmicutes, Tenericutes and Peptococcus sp., RC9_gut_group sp., and Parabacteroides sp. of the gut microbiota were significantly associated with hepatic steatosis and indicators related to oxidative stress and inflammation. Therefore, SP can mitigate hepatic lipid accumulation by regulating Nrf-2/HO-1 signaling pathways and gut microbiota. This study offers new evidence supporting the use of SP as a prebiotic treatment for NAFLD.

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沙棘多糖通过调节 Nrf-2/HO-1 通路和肠道微生物群减轻肝脂肪变性
非酒精性脂肪肝(NAFLD)正成为全球公共健康的重大威胁。沙棘(Hippophae rhamnoides L.)一直被用于传统中医学。本研究系统地探讨了沙棘多糖(SP)对高脂饮食(HFD)诱导的非酒精性脂肪肝的降脂作用,并与乳双歧杆菌 V9(B. Lactis V9)的降脂作用进行了比较。结果表明,补充 SP 后,HFD 诱导的丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平分别降低了 2.8 倍和 4.5 倍。此外,对肝脏脂质积累的缓解效果优于 B. Lactis V9。服用 SP 后,ACC 和 FASN mRNA 水平分别显著降低了 1.8 倍(P < 0.05)和 2.3 倍(P < 0.05),而 CPT1α 和 PPARα mRNA 水平则分别显著增加了 2.3 倍(P < 0.05)和 1.6 倍(P < 0.05)。SP激活磷酸化-AMPK,抑制PPARγ蛋白表达,改善血清氧化应激和炎症反应(P< 0.05)。补充SP可增加肝脏中核因子红细胞2相关因子2(Nrf-2)、血红素加氧酶-1(HO-1)和超氧化物歧化酶-2(SOD-2)的表达。此外,SP还能改善HFD引起的肠道菌群失调。肠道微生物群中的线虫属、真菌属、担子菌属和肽球菌属、RC9_肠道菌群和伞菌属与肝脏脂肪变性以及氧化应激和炎症相关指标显著相关。因此,SP 可通过调节 Nrf-2/HO-1 信号通路和肠道微生物群来缓解肝脏脂质积累。这项研究提供了新的证据,支持将 SP 用作治疗非酒精性脂肪肝的益生菌。
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来源期刊
AMB Express
AMB Express BIOTECHNOLOGY & APPLIED MICROBIOLOGY-
CiteScore
7.20
自引率
2.70%
发文量
141
审稿时长
13 weeks
期刊介绍: AMB Express is a high quality journal that brings together research in the area of Applied and Industrial Microbiology with a particular interest in ''White Biotechnology'' and ''Red Biotechnology''. The emphasis is on processes employing microorganisms, eukaryotic cell cultures or enzymes for the biosynthesis, transformation and degradation of compounds. This includes fine and bulk chemicals, polymeric compounds and enzymes or other proteins. Downstream processes are also considered. Integrated processes combining biochemical and chemical processes are also published.
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