{"title":"Microbial Ecology of Nitrate-, Selenate-, Selenite-, and Sulfate-Reducing Bacteria in a H2-Driven Bioprocess","authors":"Joshua P Boltz, Bruce E Rittmann","doi":"10.1093/femsec/fiae125","DOIUrl":null,"url":null,"abstract":"A hydrogen (H2)-based membrane biofilm reactor (H2-MBfR) can reduce electron acceptors nitrate (NO3−), selenate (SeO42−), selenite (HSeO3−), and sulfate (SO42−), which are in wastewaters from coal mining and combustion. This work presents a model to describe a H2-driven microbial community comprised of hydrogenotrophic and heterotrophic bacteria that respire NO3−, SeO42−, HSeO3−, and SO42−. The model provides mechanistic insights into the interactions between autotrophic and heterotrophic bacteria in a microbial community that is founded on H2-based autotrophy. Simulations were carried out for a range of relevant solids retention times (0.1 to 20 days) and with adequate H2-delivery capacity to reduce all electron acceptors. Bacterial activity began at an ∼0.6-day SRT, when hydrogenotrophic denitrifiers began to accumulate. Selenate-reducing and selenite-reducing hydrogenotrophs became established next, at SRTs of ∼1.2 and 2 days, respectively. Full nitrate, selenate, and selenite reductions were complete by an SRT of ∼5 days. Sulfate reduction began at an SRT of ∼10 days and was complete by ∼15 days. The desired goal of reducing nitrate, selenate, and selenite, but not sulfate, was achievable within an SRT window of 5 to 10 days. Autotrophic hydrogenotrophs dominated the active biomass, but non-active solids were a major portion of the solids, especially for an SRT ≥ 5 days.","PeriodicalId":12312,"journal":{"name":"FEMS microbiology ecology","volume":"42 1","pages":""},"PeriodicalIF":3.5000,"publicationDate":"2024-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"FEMS microbiology ecology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1093/femsec/fiae125","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A hydrogen (H2)-based membrane biofilm reactor (H2-MBfR) can reduce electron acceptors nitrate (NO3−), selenate (SeO42−), selenite (HSeO3−), and sulfate (SO42−), which are in wastewaters from coal mining and combustion. This work presents a model to describe a H2-driven microbial community comprised of hydrogenotrophic and heterotrophic bacteria that respire NO3−, SeO42−, HSeO3−, and SO42−. The model provides mechanistic insights into the interactions between autotrophic and heterotrophic bacteria in a microbial community that is founded on H2-based autotrophy. Simulations were carried out for a range of relevant solids retention times (0.1 to 20 days) and with adequate H2-delivery capacity to reduce all electron acceptors. Bacterial activity began at an ∼0.6-day SRT, when hydrogenotrophic denitrifiers began to accumulate. Selenate-reducing and selenite-reducing hydrogenotrophs became established next, at SRTs of ∼1.2 and 2 days, respectively. Full nitrate, selenate, and selenite reductions were complete by an SRT of ∼5 days. Sulfate reduction began at an SRT of ∼10 days and was complete by ∼15 days. The desired goal of reducing nitrate, selenate, and selenite, but not sulfate, was achievable within an SRT window of 5 to 10 days. Autotrophic hydrogenotrophs dominated the active biomass, but non-active solids were a major portion of the solids, especially for an SRT ≥ 5 days.
期刊介绍:
FEMS Microbiology Ecology aims to ensure efficient publication of high-quality papers that are original and provide a significant contribution to the understanding of microbial ecology. The journal contains Research Articles and MiniReviews on fundamental aspects of the ecology of microorganisms in natural soil, aquatic and atmospheric habitats, including extreme environments, and in artificial or managed environments. Research papers on pure cultures and in the areas of plant pathology and medical, food or veterinary microbiology will be published where they provide valuable generic information on microbial ecology. Papers can deal with culturable and non-culturable forms of any type of microorganism: bacteria, archaea, filamentous fungi, yeasts, protozoa, cyanobacteria, algae or viruses. In addition, the journal will publish Perspectives, Current Opinion and Controversy Articles, Commentaries and Letters to the Editor on topical issues in microbial ecology.
- Application of ecological theory to microbial ecology
- Interactions and signalling between microorganisms and with plants and animals
- Interactions between microorganisms and their physicochemical enviornment
- Microbial aspects of biogeochemical cycles and processes
- Microbial community ecology
- Phylogenetic and functional diversity of microbial communities
- Evolutionary biology of microorganisms