Multiomics Combined with Expression Pattern Analysis Reveals the Regulatory Response of Key Genes in Potato Jasmonic Acid Signaling Pathways to Cadmium Stress
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引用次数: 0
Abstract
Jasmonic acid (JA) is an endogenous phytohormone that regulates plant physiological metabolism and stress response processes, either independently or through hormone crosstalk. Our phytohormone assay and transcriptome–metabolome analysis revealed the key genes and metabolites involved in the JA pathway in response to 0–250 μM cadmium (Cd) in potato seedlings. Transcriptome gene set enrichment and gene ontology analysis indicated that JA-related genes were significantly enriched. Specifically, members from the StOPR and StJAZ gene families showed pronounced responses to Cd stress and methyl jasmonate treatment. As a negative regulatory transcription factor of the JA signaling pathway, StJAZ14 exhibited a decreasing trend under Cd stress. Yeast two-hybrid assay identified an interaction between StJAZ14 and StBZR1, which is located on the brassinolide pathway. In addition to unveiling the critical role of the JA pathway in regulating potato response to Cd stress, the functional mechanism was preliminarily explored.
期刊介绍:
The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.