Metabolomics Reveal Key Metabolic Pathway Responses to Anxiety State Regulated by Serotonin in Portunus trituberculatus.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Metabolites Pub Date : 2024-10-21 DOI:10.3390/metabo14100568
Wei Zhai, Yuanyuan Fu, Lei Liu, Xinlian Huang, Sixiang Wang
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Abstract

Background: Anxiety refers to the pathological persistence and intensification of emotional responses to danger, affecting health from psychological and physical aspects. Serotonin is an important neurotransmitter involved in the onset of anxiety.

Methods and results: To explore the biological changes in the formation of anxiety in crustaceans under the regulation of serotonin, we applied the open field-like test method for assessing anxiety states of larval Portunus trituberculatus, a highly aggressive crustacean species with a more simple neural structure compared with rodents and mammals. Compared with the control group, serotonin treatment resulted in a significant decrease in the time spent by the larvae in the central zone, suggesting anxiety-like behavior. Clonazepam treatment reversed this result and provided further evidence that the behavior of larval P. trituberculatus displayed anxiety. Moreover, a non-targeted metabolomic analysis found a significant alteration in the metabolites involved in tryptophan metabolism pathways associated with anxiety, including L-kynurenine, N-acetyl serotonin, and serotonin. These metabolites are involved in the serotonin pathway, the kynurenine pathway, and other pathways that affect anxiety through tryptophan metabolism. There were no significant differences in tryptophan metabolism levels between the control and clonazepam treatment groups.

Conclusions: Our results demonstrate the possible existence of anxiety-like behavior in the larvae of P. trituberculatus from two perspectives. Being a species with a simpler neural structure than that of mammals, the larvae of P. trituberculatus offer a convenient model for studying the mechanisms of anxiety in crustaceans.

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代谢组学揭示三疣梭子蟹受羟色胺调控的关键代谢途径对焦虑状态的反应
背景:焦虑是指对危险的情绪反应的病理性持续和强化,从心理和生理两方面影响健康。血清素是一种重要的神经递质,参与焦虑的发生:三疣梭子蟹是一种攻击性很强的甲壳类动物,其神经结构与啮齿类动物和哺乳动物相比较为简单。与对照组相比,5-羟色胺处理导致幼虫在中心区停留的时间显著减少,这表明幼虫有类似焦虑的行为。氯硝西泮处理则逆转了这一结果,进一步证明三疣梭子蟹幼虫的行为显示出焦虑。此外,非靶向代谢组分析发现,与焦虑相关的色氨酸代谢途径中的代谢物发生了显著变化,包括 L-犬尿氨酸、N-乙酰羟色胺和羟色胺。这些代谢物参与了血清素途径、犬尿氨酸途径以及其他通过色氨酸代谢影响焦虑的途径。对照组和氯硝西泮治疗组的色氨酸代谢水平没有明显差异:我们的研究结果从两个方面证明了三疣梭子蟹幼虫可能存在类似焦虑的行为。作为一种神经结构比哺乳动物简单的物种,三疣梭子蟹幼体为研究甲壳类动物的焦虑机制提供了一个方便的模型。
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来源期刊
Metabolites
Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍: Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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