Periostin Is a Disulfide-Bonded Homodimer and Forms a Complex with Fibronectin in the Human Skin.

IF 2.9 3区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry Biochemistry Pub Date : 2024-10-01 DOI:10.1021/acs.biochem.4c00240

Christian E Rusbjerg-Weberskov, Carsten Scavenius, Jan J Enghild, Nadia Sukusu Nielsen

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Abstract

The protein periostin is a matricellular protein that is expressed in connective tissue. It is composed of five globular domains arranged in an elongated structure with an extensive disordered C-terminal tail. Periostin contains 11 cysteine residues, of which one is unpaired and the rest form five intramolecular disulfide bonds. Periostin plays an important role during wound healing and is also involved in driving the inflammatory state in atopic diseases. This study provides a comprehensive biochemical characterization of periostin in human skin and in dermal and pulmonary fibroblasts in vitro. Through the application of Western blotting, co-immunoprecipitation, and LC-MS/MS, we show for the first time that periostin is a disulfide-bonded homodimer and engages in a novel disulfide-bonded complex with fibronectin both in vivo and in vitro. This inherent characteristic of periostin holds the potential to redefine our approach to exploring and understanding its functional role in future research endeavors.

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包膜粘连蛋白是一种二硫键同源二聚体，在人体皮肤中与纤连蛋白形成复合物。

骨膜蛋白是一种在结缔组织中表达的母细胞蛋白。它由五个球状结构域组成，这些结构域呈拉长结构，C-末端有大量无序的尾部。表皮生长因子含有 11 个半胱氨酸残基，其中一个未配对，其余的形成五个分子内二硫键。表皮生长因子在伤口愈合过程中发挥着重要作用，也参与了特应性疾病炎症状态的驱动。本研究对人体皮肤以及体外真皮和肺成纤维细胞中的表皮生长因子进行了全面的生化鉴定。通过 Western 印迹、共免疫沉淀和 LC-MS/MS，我们首次发现了包膜组织蛋白是一种二硫键同源二聚体，并在体内和体外与纤维连接蛋白形成了一种新型的二硫键复合物。包柔素的这一固有特性有可能重新定义我们在未来研究工作中探索和理解其功能作用的方法。

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来源期刊

Biochemistry Biochemistry 生物-生化与分子生物学

CiteScore

5.50

自引率

3.40%

发文量

336

审稿时长

1-2 weeks

期刊介绍： Biochemistry provides an international forum for publishing exceptional, rigorous, high-impact research across all of biological chemistry. This broad scope includes studies on the chemical, physical, mechanistic, and/or structural basis of biological or cell function, and encompasses the fields of chemical biology, synthetic biology, disease biology, cell biology, nucleic acid biology, neuroscience, structural biology, and biophysics. In addition to traditional Research Articles, Biochemistry also publishes Communications, Viewpoints, and Perspectives, as well as From the Bench articles that report new methods of particular interest to the biological chemistry community.