Effect of Probiotics on Improving Intestinal Mucosal Permeability and Inflammation after Surgery.

IF 3.4 3区 医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Gut and Liver Pub Date : 2024-09-27 DOI:10.5009/gnl240170
Min-Jae Kim, Young Ju Lee, Zahid Hussain, Hyojin Park
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Abstract

Background/aims: We explored the mechanisms underlying the improvement of postoperative ileus (POI) following probiotic pretreatment. We assessed intestinal permeability, inflammation, tight junction (TJ) protein expression in the gut epithelium, and plasma interleukin (IL)-17 levels in a guinea pig model of POI.

Methods: Guinea pigs were divided into control, POI, and probiotic groups. The POI and probiotic groups underwent surgery, but the probiotic group received probiotics before the procedure. The ileum and proximal colon were harvested. Intestinal permeability was measured via horseradish peroxidase permeability. Inflammation was evaluated via leukocyte count in the intestinal wall muscle layer, and calprotectin expression in each intestinal wall layer was analyzed immunohistochemically. TJ proteins were analyzed using immunohistochemical staining, and plasma IL-17 levels were measured using an enzyme-linked immunosorbent assay.

Results: The POI group exhibited increased intestinal permeability and inflammation, whereas probiotic pretreatment reduced the extent of these POI-induced changes. Probiotics restored the expression of TJ proteins occludin and zonula occludens-1 in the proximal colon, which were increased in the POI group. Calprotectin expression significantly increased in the muscle layer of the POI group and was downregulated in the probiotic group; however, no distinct differences were observed between the mucosal and submucosal layers. Plasma IL-17 levels did not significantly differ among the groups.

Conclusions: Probiotic pretreatment may relieve POI by reducing intestinal permeability and inflammation and TJ protein expression in the gut epithelium. These findings suggest a potential therapeutic approach for POI management.

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益生菌对改善手术后肠道黏膜通透性和炎症的影响
背景/目的:我们探讨了益生菌预处理改善术后回肠梗阻(POI)的机制。我们评估了豚鼠 POI 模型的肠道通透性、炎症、肠道上皮细胞中紧密连接(TJ)蛋白的表达以及血浆白细胞介素(IL)-17 水平:方法:将豚鼠分为对照组、POI 组和益生菌组。方法:豚鼠分为对照组和益生菌组,对照组和益生菌组均接受手术,但益生菌组在手术前服用益生菌。采集回肠和近端结肠。通过辣根过氧化物酶渗透性测量肠道渗透性。炎症通过肠壁肌层的白细胞计数进行评估,肠壁各层的钙蛋白表达通过免疫组化进行分析。使用免疫组化染色法分析 TJ 蛋白,使用酶联免疫吸附法测定血浆 IL-17 水平:结果:POI 组的肠道通透性和炎症增加,而益生菌预处理降低了 POI 引起的这些变化的程度。益生菌恢复了近端结肠中 TJ 蛋白闭塞素和 zonula occludens-1 的表达,而 POI 组中这两种蛋白的表达有所增加。POI 组肌肉层的钙蛋白表达明显增加,而益生菌组的钙蛋白表达则有所下降;不过,在粘膜层和粘膜下层之间没有观察到明显的差异。血浆 IL-17 水平在各组间无明显差异:结论:益生菌预处理可降低肠道通透性、炎症和肠道上皮细胞中 TJ 蛋白的表达,从而缓解 POI。这些研究结果表明,益生菌是治疗 POI 的一种潜在方法。
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来源期刊
Gut and Liver
Gut and Liver 医学-胃肠肝病学
CiteScore
7.50
自引率
8.80%
发文量
119
审稿时长
6-12 weeks
期刊介绍: Gut and Liver is an international journal of gastroenterology, focusing on the gastrointestinal tract, liver, biliary tree, pancreas, motility, and neurogastroenterology. Gut and Liver delivers up-to-date, authoritative papers on both clinical and research-based topics in gastroenterology. The Journal publishes original articles, case reports, brief communications, letters to the editor and invited review articles in the field of gastroenterology. The Journal is operated by internationally renowned editorial boards and designed to provide a global opportunity to promote academic developments in the field of gastroenterology and hepatology. Gut and Liver is jointly owned and operated by 8 affiliated societies in the field of gastroenterology, namely: the Korean Society of Gastroenterology, the Korean Society of Gastrointestinal Endoscopy, the Korean Society of Neurogastroenterology and Motility, the Korean College of Helicobacter and Upper Gastrointestinal Research, the Korean Association for the Study of Intestinal Diseases, the Korean Association for the Study of the Liver, the Korean Pancreatobiliary Association, and the Korean Society of Gastrointestinal Cancer.
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