Acetaminophen production in the edible, filamentous cyanobacterium Arthrospira platensis.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology and Bioengineering Pub Date : 2024-10-11 DOI:10.1002/bit.28858

Jacob M Hilzinger, Skyler Friedline, Divya Sivanandan, Ya-Fang Cheng, Shunsuke Yamazaki, Douglas S Clark, Jeffrey M Skerker, Adam P Arkin

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Abstract

Spirulina is the common name for the edible, nonheterocystous, filamentous cyanobacterium Arthrospira platensis that is grown industrially as a food supplement, animal feedstock, and pigment source. Although there are many applications for engineering this organism, until recently no genetic tools or reproducible transformation methods have been published. While recent work showed the production of a diversity of proteins in A. platensis, including single-domain antibodies for oral delivery, there remains a need for a modular, characterized genetic toolkit. Here, we independently establish a reproducible method for the transformation of A. platensis and engineer this bacterium to produce acetaminophen as proof-of-concept for small molecule production in an edible host. This work opens A. platensis to the wider scientific community for future engineering as a functional food for nutritional enhancement, modification of organoleptic traits, and production of pharmaceuticals for oral delivery.

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可食用丝状蓝藻 Arthrospira platensis 中的对乙酰氨基酚生产。

螺旋藻是一种可食用的非单胞丝状蓝藻的俗称，在工业上被用作食品补充剂、动物饲料和色素来源。虽然这种生物的工程应用很多，但直到最近，还没有遗传工具或可重复的转化方法问世。虽然最近的研究表明，A. platensis 可以生产多种蛋白质，包括用于口服给药的单域抗体，但仍然需要一个模块化、特征化的基因工具包。在这里，我们独立地建立了一种可重复的方法来转化 A. platensis，并设计这种细菌来生产对乙酰氨基酚，作为在可食用宿主中生产小分子的概念验证。这项工作为更广泛的科学界打开了大门，未来可将 Platensis 改造成一种功能性食品，用于增强营养、改变感官性状和生产口服给药的药物。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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