{"title":"Swelling Induced Hardening and Degradation Induced Slow-Expansion Hydrogel for a Modified Cervical Spinal Cord Compression Animal Model.","authors":"Rui Chen, Jiaxin Shi, Tianyuan Zhao, Shuyang Li, Haoxiang Wang, Jun Xu, Baohua Guo, Feifei Zhou","doi":"10.1021/acsami.4c12023","DOIUrl":null,"url":null,"abstract":"<p><p>This study presents the development of a polyurethane hydrogel (PUG) for use in a chronic cervical spinal cord compression animal model, leveraging microphase separation and dynamic covalent bonds to achieve swelling induced hardening and degradation-induced slow expansion. PUG-SS and PUG-SS-60% were synthesized with varying disulfide bond concentrations, offering controllable degradation rates and mechanical properties. The hydrogels demonstrated significant swelling-induced hardening and maintained compression above the cervical spinal cord's intrinsic modulus. MRI and histopathological analyses confirmed effective and sustained spinal cord compression, with PUG-SS-60% showing prolonged effects. Behavioral tests, including the BBB locomotor scale, von Frey pain test, and catwalk gait analysis, indicated quicker motor function recovery with PUG-SS and sustained compression with PUG-SS-60%. In vitro cytotoxicity assays showed no significant hydrogel-induced cell death. This study underscores the potential of PUG-SS-60% for providing controlled, sustained compression in chronic spinal cord compression models, paving the way for advanced nonsurgical treatment strategies and improved understanding of degenerative cervical myelopathy (DCM) pathology.</p>","PeriodicalId":5,"journal":{"name":"ACS Applied Materials & Interfaces","volume":null,"pages":null},"PeriodicalIF":8.3000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Materials & Interfaces","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acsami.4c12023","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
This study presents the development of a polyurethane hydrogel (PUG) for use in a chronic cervical spinal cord compression animal model, leveraging microphase separation and dynamic covalent bonds to achieve swelling induced hardening and degradation-induced slow expansion. PUG-SS and PUG-SS-60% were synthesized with varying disulfide bond concentrations, offering controllable degradation rates and mechanical properties. The hydrogels demonstrated significant swelling-induced hardening and maintained compression above the cervical spinal cord's intrinsic modulus. MRI and histopathological analyses confirmed effective and sustained spinal cord compression, with PUG-SS-60% showing prolonged effects. Behavioral tests, including the BBB locomotor scale, von Frey pain test, and catwalk gait analysis, indicated quicker motor function recovery with PUG-SS and sustained compression with PUG-SS-60%. In vitro cytotoxicity assays showed no significant hydrogel-induced cell death. This study underscores the potential of PUG-SS-60% for providing controlled, sustained compression in chronic spinal cord compression models, paving the way for advanced nonsurgical treatment strategies and improved understanding of degenerative cervical myelopathy (DCM) pathology.
期刊介绍:
ACS Applied Materials & Interfaces is a leading interdisciplinary journal that brings together chemists, engineers, physicists, and biologists to explore the development and utilization of newly-discovered materials and interfacial processes for specific applications. Our journal has experienced remarkable growth since its establishment in 2009, both in terms of the number of articles published and the impact of the research showcased. We are proud to foster a truly global community, with the majority of published articles originating from outside the United States, reflecting the rapid growth of applied research worldwide.