{"title":"Restricted Intramolecular Rotation: A Dual Fluorescence Response to Hg<sup>2+</sup> Quenching and Ag<sup>+</sup> Enhancement in Live Rhizoctonia Solani Cells.","authors":"Ramanjaneyulu Mala, Dhakshinamurthy Divya, Manivannan Nandhagopal, Sathiah Thennarasu","doi":"10.1007/s10895-024-03976-3","DOIUrl":null,"url":null,"abstract":"<p><p>A novel imidazo[1,2-a]pyridine derivative probe (R) was designed, synthesized, and characterized via various characterization techniques, such as ESI-MS, <sup>1</sup>H NMR, <sup>13</sup>C NMR, and Dept-135 NMR. The data obtained from single-crystal XRD reveal that probe R has a coplanar configuration and is part of the monoclinic crystal system, designated the P2(1)/n space group. The fluorescence of (R) is further enhanced by silver (I) ions. On the other hand, Hg<sup>2+</sup> significantly quenches the fluorescence of probe R. The presence of other common metal ions does not influence the fluorescence of probe R in the CH<sub>3</sub>CN: H<sub>2</sub>O (1:1) mixture, as they neither increase nor quench it. From the fluorescence enhancement data, the low detection limit (LOD) for Ag + ions was determined to be 1.24 × 10<sup>- 8</sup> M, whereas the quenching caused by Hg<sup>2+</sup> resulted in an LOD of 5.69 × 10<sup>- 9</sup> M. The complex of probe R with Ag<sup>+</sup>/Hg<sup>2+</sup> exhibited a 1:1 stoichiometry, as confirmed by mass spectrometry and a Job plot. Single-crystal XRD analysis of R and its complex with Hg<sup>2+</sup> revealed a loss of coplanarity, which confirmed their nonfluorescent behavior. We present a promising application of probe R in visualizing living Rhizoctonia oryzae cells exposed to silver (I) and mercury (II) ions.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-024-03976-3","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
A novel imidazo[1,2-a]pyridine derivative probe (R) was designed, synthesized, and characterized via various characterization techniques, such as ESI-MS, 1H NMR, 13C NMR, and Dept-135 NMR. The data obtained from single-crystal XRD reveal that probe R has a coplanar configuration and is part of the monoclinic crystal system, designated the P2(1)/n space group. The fluorescence of (R) is further enhanced by silver (I) ions. On the other hand, Hg2+ significantly quenches the fluorescence of probe R. The presence of other common metal ions does not influence the fluorescence of probe R in the CH3CN: H2O (1:1) mixture, as they neither increase nor quench it. From the fluorescence enhancement data, the low detection limit (LOD) for Ag + ions was determined to be 1.24 × 10- 8 M, whereas the quenching caused by Hg2+ resulted in an LOD of 5.69 × 10- 9 M. The complex of probe R with Ag+/Hg2+ exhibited a 1:1 stoichiometry, as confirmed by mass spectrometry and a Job plot. Single-crystal XRD analysis of R and its complex with Hg2+ revealed a loss of coplanarity, which confirmed their nonfluorescent behavior. We present a promising application of probe R in visualizing living Rhizoctonia oryzae cells exposed to silver (I) and mercury (II) ions.
期刊介绍:
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.