Restricted Intramolecular Rotation: A Dual Fluorescence Response to Hg2+ Quenching and Ag+ Enhancement in Live Rhizoctonia Solani Cells.

IF 2.6 4区 化学 Q2 BIOCHEMICAL RESEARCH METHODS Journal of Fluorescence Pub Date : 2024-10-16 DOI:10.1007/s10895-024-03976-3
Ramanjaneyulu Mala, Dhakshinamurthy Divya, Manivannan Nandhagopal, Sathiah Thennarasu
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Abstract

A novel imidazo[1,2-a]pyridine derivative probe (R) was designed, synthesized, and characterized via various characterization techniques, such as ESI-MS, 1H NMR, 13C NMR, and Dept-135 NMR. The data obtained from single-crystal XRD reveal that probe R has a coplanar configuration and is part of the monoclinic crystal system, designated the P2(1)/n space group. The fluorescence of (R) is further enhanced by silver (I) ions. On the other hand, Hg2+ significantly quenches the fluorescence of probe R. The presence of other common metal ions does not influence the fluorescence of probe R in the CH3CN: H2O (1:1) mixture, as they neither increase nor quench it. From the fluorescence enhancement data, the low detection limit (LOD) for Ag + ions was determined to be 1.24 × 10- 8 M, whereas the quenching caused by Hg2+ resulted in an LOD of 5.69 × 10- 9 M. The complex of probe R with Ag+/Hg2+ exhibited a 1:1 stoichiometry, as confirmed by mass spectrometry and a Job plot. Single-crystal XRD analysis of R and its complex with Hg2+ revealed a loss of coplanarity, which confirmed their nonfluorescent behavior. We present a promising application of probe R in visualizing living Rhizoctonia oryzae cells exposed to silver (I) and mercury (II) ions.

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分子内旋转受限:活体根瘤菌细胞中对 Hg2+ 淬灭和 Ag+ 增强的双重荧光反应。
研究人员设计、合成了一种新型咪唑并[1,2-a]吡啶衍生物探针(R),并通过各种表征技术,如 ESI-MS、1H NMR、13C NMR 和 Dept-135 NMR 对其进行了表征。单晶 XRD 数据显示,探针 R 具有共面构型,属于单斜晶系,即 P2(1)/n 空间群。银(I)离子进一步增强了 (R) 的荧光。其他常见金属离子的存在不会影响探针 R 在 CH3CN:H2O(1:1)混合物中的荧光,因为它们既不会增强也不会淬灭荧光。根据荧光增强数据,Ag + 离子的低检测限 (LOD) 被确定为 1.24 × 10- 8 M,而 Hg2+ 引起的淬灭导致低检测限为 5.69 × 10- 9 M。探针 R 及其与 Hg2+ 的复合物的单晶 XRD 分析显示共面性消失,这证实了它们的非荧光行为。我们介绍了探针 R 在观察暴露于银(I)和汞(II)离子的根瘤菌活细胞中的应用前景。
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来源期刊
Journal of Fluorescence
Journal of Fluorescence 化学-分析化学
CiteScore
4.60
自引率
7.40%
发文量
203
审稿时长
5.4 months
期刊介绍: Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.
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