{"title":"Genomic insights into qnrVC1 gene located on an IncP6 plasmid carried by multidrug resistant Pseudomonas aeruginosa from clinical asinine isolates","authors":"","doi":"10.1016/j.vetmic.2024.110285","DOIUrl":null,"url":null,"abstract":"<div><div><em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) is an opportunistic pathogen, causing significant global health threat due to its antimicrobial resistance. Among equines, <em>P. aeruginosa</em> can cause infections, particularly in the reproductive tract, leading to reproductive failure. Multidrug-resistant (MDR) <em>P. aeruginosa</em> has been a major concern in animal husbandry, including the donkey industry. The study aims to elucidate the phylogenetic relationship of <em>P. aeruginosa</em> strains isolated from donkeys with endometritis farmed in a large intensive unit in Hebei Province, China. Genes coding for multiple antimicrobial resistances were predicted by whole genomic sequencing. Multilocus sequence typing (MLST) revealed that all strains belonged to the same sequence type (ST1058). An IncP6 plasmid encoding the <em>qnrVC1</em> gene, associated with quinolone resistance, was identified. Comparative genomic analysis illustrated the characteristics of the strains and genetic context of <em>qnrVC1</em>. This study is the first to report that these MDR <em>P. aeruginosa</em> asinine strains exhibited high levels of antimicrobial and metal resistance conferred by a <em>qnrVC1</em>-carrying plasmid. Additionally, <em>P. aeruginosa</em> strains with integrated mega-plasmids were identified. From a One Health perspective, the study underlined the significance of monitoring antimicrobial resistance genes in food animals, including donkeys.</div></div>","PeriodicalId":23551,"journal":{"name":"Veterinary microbiology","volume":null,"pages":null},"PeriodicalIF":2.4000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinary microbiology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378113524003079","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen, causing significant global health threat due to its antimicrobial resistance. Among equines, P. aeruginosa can cause infections, particularly in the reproductive tract, leading to reproductive failure. Multidrug-resistant (MDR) P. aeruginosa has been a major concern in animal husbandry, including the donkey industry. The study aims to elucidate the phylogenetic relationship of P. aeruginosa strains isolated from donkeys with endometritis farmed in a large intensive unit in Hebei Province, China. Genes coding for multiple antimicrobial resistances were predicted by whole genomic sequencing. Multilocus sequence typing (MLST) revealed that all strains belonged to the same sequence type (ST1058). An IncP6 plasmid encoding the qnrVC1 gene, associated with quinolone resistance, was identified. Comparative genomic analysis illustrated the characteristics of the strains and genetic context of qnrVC1. This study is the first to report that these MDR P. aeruginosa asinine strains exhibited high levels of antimicrobial and metal resistance conferred by a qnrVC1-carrying plasmid. Additionally, P. aeruginosa strains with integrated mega-plasmids were identified. From a One Health perspective, the study underlined the significance of monitoring antimicrobial resistance genes in food animals, including donkeys.
期刊介绍：
Veterinary Microbiology is concerned with microbial (bacterial, fungal, viral) diseases of domesticated vertebrate animals (livestock, companion animals, fur-bearing animals, game, poultry, fish) that supply food, other useful products or companionship. In addition, Microbial diseases of wild animals living in captivity, or as members of the feral fauna will also be considered if the infections are of interest because of their interrelation with humans (zoonoses) and/or domestic animals. Studies of antimicrobial resistance are also included, provided that the results represent a substantial advance in knowledge. Authors are strongly encouraged to read - prior to submission - the Editorials (''Scope or cope'' and ''Scope or cope II'') published previously in the journal. The Editors reserve the right to suggest submission to another journal for those papers which they feel would be more appropriate for consideration by that journal.
Original research papers of high quality and novelty on aspects of control, host response, molecular biology, pathogenesis, prevention, and treatment of microbial diseases of animals are published. Papers dealing primarily with immunology, epidemiology, molecular biology and antiviral or microbial agents will only be considered if they demonstrate a clear impact on a disease. Papers focusing solely on diagnostic techniques (such as another PCR protocol or ELISA) will not be published - focus should be on a microorganism and not on a particular technique. Papers only reporting microbial sequences, transcriptomics data, or proteomics data will not be considered unless the results represent a substantial advance in knowledge.
Drug trial papers will be considered if they have general application or significance. Papers on the identification of microorganisms will also be considered, but detailed taxonomic studies do not fall within the scope of the journal. Case reports will not be published, unless they have general application or contain novel aspects. Papers of geographically limited interest, which repeat what had been established elsewhere will not be considered. The readership of the journal is global.
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