Prevalence and Risk Factors of β-Lactamase Genes of Extended-Spectrum β-Lactamases-Producing Escherichia coli From Dairy Farm Environments of Haryana, India.

IF 2.3 Q2 PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH Environmental Health Insights Pub Date : 2024-10-31 eCollection Date: 2024-01-01 DOI:10.1177/11786302241296694
Sarin Kamboj, Jinu Manoj, Jasleen Kaur, Mahavir Singh, Rajesh Chhabra
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Abstract

Presence of extended-spectrum β-lactamases (ESBL)-producing Enterobacteriaceae in the dairy farm environment and food chain could be a possible interface for the exchange of antimicrobial resistance genes between humans and animals. A total of 600 samples comprised of raw bovine milk, faeces, feed, environmental swabs and water samples from 20 different bovine dairy farms in and around Hisar city, Haryana, India were analysed for presence of ESBL encoding genes. Out of 240 isolates of Escherichia coli obtained, 74 isolates were found to be ESBL producers. Maximum number of ESBL isolates were found from faeces (40.5%) followed by raw milk (37.8%) and environmental swabs (17.5%). Most of the ESBL E. coli isolates were sensitive to chloramphenicol (82.4%) and gentamicin (77.0%) antibiotics. The bla CTX-M gene was found to be most prevalent (52.0%) followed by bla TEM (9.45%) while bla SHV gene alone was not detected in any sample by simplex PCR. However, the co-expression of blaCTX-M + blaTEM (21.6%) and blaCTX-M + blaSHV (4.05%) genes were also observed. The housing system, milking method and the hygienic mangement practices followed at farm level are found to be significant risk factors of ESBL-producing E. coli in dairy farms of Haryana.

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印度哈里亚纳邦奶牛场环境中产广谱β-内酰胺酶大肠埃希氏菌β-内酰胺酶基因的流行率和风险因素。
奶牛场环境和食物链中存在产广谱β-内酰胺酶(ESBL)的肠杆菌科细菌,这可能是人与动物之间交换抗菌药耐药性基因的一个界面。研究人员对来自印度哈里亚纳邦希萨尔市及周边地区 20 个不同奶牛场的共计 600 份样品(包括生牛乳、粪便、饲料、环境拭子和水样)进行了分析,以检测是否存在 ESBL 编码基因。在获得的 240 株大肠埃希菌分离物中,发现有 74 株分离物产生了 ESBL。从粪便(40.5%)中发现的 ESBL 分离物最多,其次是生奶(37.8%)和环境拭子(17.5%)。大多数 ESBL 大肠杆菌分离物对氯霉素(82.4%)和庆大霉素(77.0%)抗生素敏感。bla CTX-M 基因最普遍(52.0%),其次是 bla TEM(9.45%),而通过单纯聚合酶链式反应,没有在任何样本中检测到单独的 bla SHV 基因。然而,也观察到 blaCTX-M + blaTEM(21.6%)和 blaCTX-M + blaSHV(4.05%)基因的共同表达。研究发现,哈里亚纳邦奶牛场的饲养系统、挤奶方法和卫生管理措施是产生 ESBL 大肠杆菌的重要风险因素。
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来源期刊
Environmental Health Insights
Environmental Health Insights PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH-
CiteScore
3.20
自引率
22.20%
发文量
97
审稿时长
8 weeks
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