Metabolic interactions of limosilactobacillus reuteri ZJ625 and ligilactobacillus salivarius ZJ614 in Co-culture: implications for multi-strain probiotics.

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC ACS Applied Electronic Materials Pub Date : 2024-11-07 DOI:10.1093/jambio/lxae264

Iliya Dauda Kwoji, Moses Okpeku, Olayinka Ayobami Aiyegoro, Matthew Adekunle Adeleke

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Abstract

Aims: Limosilactobacillus reuteri ZJ625 and Ligilactobacillus salivarius ZJ614 are potential probiotic bacteria. The mechanisms of enhanced benefits by muti-strain probiotics are yet fully understood. We elucidated the influence of co-culturing on the metabolite profiles of L. reuteri ZJ625 and L. salivarius ZJ614 to decipher the impacts of co-culturing on metabolic interactions between the strains.

Methods and results: L. reuteri ZJ625 and L. salivarius ZJ614 were grown in single and co-cultures in defined media. Bacterial cell metabolites were extracted at the mid-stationary growth phase and analysed using two-dimensional gas-column time-of-flight-mass spectrometry (GCxGC- TOFMS). Mass-spectral data was preprocessed and analysed using unsupervised and supervised methods based on the group allocations. A total of 1387 metabolites were identified, with 18.31% significant metabolites (p < 0.05) and 10.17% differential metabolites (p < 0.05, VIP > 1). The differential metabolites identified include arabinofuranose, methyl-galactoside, N-acetyl-glutamic acid, phosphoric acid, and decanoic acid. The metabolites impacted carbohydrate and amino-sugar metabolism.

Conclusion: co-culturing of L. reuteri ZJ625 and L. salivarius ZJ614 influenced the metabolite profiles of the strains and impacted metabolic/biosynthetic pathways, indicating cell-to-cell interactions between the strains.

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LIMOSILACTORACILLUS REUTERI ZJ625 和 LIGILACTORACILLUS SALIVARIUS ZJ614 在共培养中的代谢相互作用：对多菌株益生菌的影响。

目的：Limosilactobacillus reuteri ZJ625 和 Ligilactobacillus salivarius ZJ614 是潜在的益生菌。变异菌株益生菌增强益处的机制尚未完全清楚。我们阐明了共培养对L. reuteri ZJ625和L. salivarius ZJ614代谢物谱的影响，以破译共培养对菌株间代谢相互作用的影响：L. reuteri ZJ625 和 L. salivarius ZJ614 在确定的培养基中进行单培养和共培养。在生长中期提取细菌细胞代谢物，并使用二维气柱飞行时间质谱法（GCxGC- TOFMS）进行分析。质谱数据经过预处理，并根据分组分配使用无监督和有监督方法进行分析。共鉴定出 1387 种代谢物，其中 18.31% 为重要代谢物（p 1）。鉴定出的差异代谢物包括阿拉伯呋喃糖、甲基半乳糖苷、N-乙酰谷氨酸、磷酸和癸酸。结论：L. reuteri ZJ625 和 L. salivarius ZJ614 的共培养影响了菌株的代谢物谱，并影响了代谢/生物合成途径，表明菌株之间存在细胞间相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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