Seasonal Variation in ATP-Induced Retinal Damage in the Cone-Dominant 13-Lined Ground Squirrel.

IF 2.6 3区医学 Q2 OPHTHALMOLOGY Translational Vision Science & Technology Pub Date : 2024-11-04 DOI:10.1167/tvst.13.11.5

Owen R Bowie, Hannah M Follett, Ching Tzu Yu, Chloe Guillaume, Phyllis M Summerfelt, Nicole Manfredonia, Jenna Grieshop, Dana K Merriman, Sergey Tarima, Joseph Carroll

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Abstract

Purpose: To examine whether time of year (relative to hibernation emergence) influences the retinal degenerative effects of intravitreal injection of adenosine triphosphate (ATP) in the 13-lined ground squirrel (13-LGS).

Methods: Eighteen (9 male, 9 female) 13-LGS in three experimental cohorts (early season, mid-season, late season) (n = 6 each) underwent baseline imaging using scanning light ophthalmoscopy (SLO) and optical coherence tomography (OCT). Animals then received a 10-µL intravitreal injection of 0.723 M ATP, followed by OCT and SLO imaging at 3, 10, and 21 days. Adaptive optics SLO (AOSLO) was performed in animals without retinal damage after the 21-day follow-up. Retinal thickness, choroidal thickness, and cone density measures were compared to values from wild-type controls (n = 12).

Results: Five animals (four early season, one late season) showed retinal damage post-ATP injection (Fisher's exact test, P = 0.065). Animals with retinal damage displayed areas of disrupted retinal lamination on OCT. Any changes in OCT thickness were generally present on initial follow-up and resolved at later time points. Follow-up imaging with AOSLO on animals without retinal damage showed no significant differences in the cone mosaic topography from control eyes. Axial length was increased in mid-/late-season cohorts relative to early season (P = 0.0025 and P = 0.0007).

Conclusions: In this pilot study, the 13-LGS appears more susceptible to ATP-induced retinal damage during the early season. Future studies adjusting dose based on ocular biometry may help elucidate the impact of time of year on chemical response.

Translational relevance: Consideration of ocular biometry in this and other animal models is merited when using intravitreal methods of chemical administration.

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锥形 13 线地松鼠 ATP 诱导视网膜损伤的季节性变化

目的：研究一年中的时间（相对于冬眠开始时间）是否会影响 13 线地松鼠（13-LGS）玻璃体内注射三磷酸腺苷（ATP）对视网膜变性的影响：三个实验群组（季初、季中、季末）中的 18 只（9 雄性，9 雌性）13-LGS（n = 6 只）使用扫描光眼底镜（SLO）和光学相干断层扫描（OCT）进行了基线成像。然后，动物接受 10 微升 0.723 M ATP 玻璃体内注射，并在 3 天、10 天和 21 天时接受 OCT 和 SLO 成像检查。21 天随访后，对无视网膜损伤的动物进行自适应光学 SLO（AOSLO）检查。将视网膜厚度、脉络膜厚度和视锥密度测量值与野生型对照组（n = 12）的值进行比较：结果：注射ATP后，5只动物（4只早期，1只晚期）出现视网膜损伤（费雪精确检验，P = 0.065）。视网膜受损的动物在 OCT 上显示出视网膜层的破坏区域。OCT 厚度的任何变化一般都出现在最初的随访中，并在随后的时间点得到缓解。用 AOSLO 对无视网膜损伤的动物进行随访成像显示，锥体镶嵌地形图与对照眼无明显差异。中/晚季节组群的轴长相对于早季节组群有所增加（P = 0.0025 和 P = 0.0007）：在这项试验性研究中，13-LGS 似乎更容易在早期季节受到 ATP 引起的视网膜损伤。根据眼部生物测量学调整剂量的未来研究可能有助于阐明一年中的时间对化学反应的影响：在使用玻璃体内给药方法时，应考虑该动物模型和其他动物模型的眼部生物测量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Translational Vision Science & Technology Engineering-Biomedical Engineering

CiteScore

5.70

自引率

3.30%

发文量

346

审稿时长

25 weeks

期刊介绍： Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.