Inflammation-attenuating effect of carbon dioxide versus room-air environment in a rat laparotomy model.

IF 2.4 2区医学 Q2 SURGERY Surgical Endoscopy And Other Interventional Techniques Pub Date : 2024-11-15 DOI:10.1007/s00464-024-11388-4

Petros Ypsilantis, Ioanna Stylianaki, Fotini Papachristou, Panagiotis Papatheodorou, Christos Svoronos, Konstantinos Spyridakis, Michael Margaritis, Ifigenia Ypsilantou, Konstantinos Ypsilantis, Nikolaos Papaioannou, Anastasios Karayiannakis, Michael Pitiakoudis

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Abstract

Background: The mechanism by which laparoscopic operations induce lower post-operative inflammatory response compared to open surgery was investigated with regard to the effect of the type of gas environment.

Methods: Rats were subjected to midline laparotomy at either CO₂ (group CO₂) or room-air environment (group Air) or to anesthesia only (group Control) under atmospheric pressure conditions. At various timepoints after surgery (1, 3, 6, 24, or 48 h), the expression of inflammation biomarkers interleukin-6 (IL-6), tumor necrosis factor-α (TNFα), and nuclear factor-κΒ (NFκΒ) were assessed immunohistochemically in tissue samples excised from the liver, intestine, and kidneys, accompanied by histopathologic analysis, and their levels were measured by ELISA in blood samples.

Results: Tissue expression of IL-6, TNFα, and NFκΒ was downregulated in the liver and intestine in group CO₂ compared to group Air and in the kidneys in group Air compared to group CO₂. However, no differences were noted among groups regarding the histopathologic score of organ tissues and the blood serum levels of inflammation biomarkers.

Conclusion: Post-operative local inflammatory response was lower in intra-peritoneal organs of rats subjected to laparotomy at CO₂ rather than room-air environment under atmospheric pressure conditions.

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二氧化碳与室内空气环境对大鼠腹腔手术模型的炎症抑制作用

背景：与开腹手术相比，腹腔镜手术诱发术后炎症反应较低：研究了腹腔镜手术诱发术后炎症反应低于开腹手术的机制与气体环境类型的影响：方法：大鼠在二氧化碳（CO2 组）或室内空气环境（空气组）下接受中线开腹手术，或在常压条件下仅接受麻醉（对照组）。在手术后的不同时间点（1、3、6、24 或 48 h），对肝脏、肠道和肾脏切除的组织样本中的炎症生物标志物白细胞介素-6（IL-6）、肿瘤坏死因子-α（TNFα）和核因子-κΒ（NFκΒ）的表达进行免疫组化评估，并进行组织病理学分析，同时用 ELISA 方法测定血液样本中的含量：结果：与空气组相比，IL-6、TNFα和NFκΒ在二氧化碳组肝脏和肠道中的组织表达下调；与二氧化碳组相比，IL-6、TNFα和NFκΒ在空气组肾脏中的组织表达下调。然而，器官组织的组织病理学评分和血清中的炎症生物标志物水平在各组之间没有差异：结论：在二氧化碳环境下进行腹腔手术的大鼠，术后腹腔内器官的局部炎症反应低于常压条件下的室空气环境。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Surgical Endoscopy And Other Interventional Techniques 医学-外科

CiteScore

6.10

自引率

12.90%

发文量

890

审稿时长

6 months

期刊介绍： Uniquely positioned at the interface between various medical and surgical disciplines, Surgical Endoscopy serves as a focal point for the international surgical community to exchange information on practice, theory, and research. Topics covered in the journal include: -Surgical aspects of: Interventional endoscopy, Ultrasound, Other techniques in the fields of gastroenterology, obstetrics, gynecology, and urology, -Gastroenterologic surgery -Thoracic surgery -Traumatic surgery -Orthopedic surgery -Pediatric surgery