Novel Green Method for Enhancing the Extraction Efficiency of Bioactive Compounds: Falcaria vulgaris Extract

Abstract

The aim of the present research is to investigate the effect of ohmic-assisted extraction (OAE) parameters on the Falcaria vulgaris extract (FVE) properties. Various parameters, including ethanol-to-water ratio (0%–100%), voltage gradient (10–30 V/cm), temperature (30°C–60°C), and extraction time (10–30 min), were applied as independent factors. Physicochemical process, antimicrobial properties, and energy analysis were accomplished to achieve OAE optimization. Results indicated that extraction yield (EY) and energy consumption (EC) significantly increased with increasing the voltage gradient and extraction time (p < 0.05). Also, EY significantly increased with increasing of process temperature and the ethanol concentration in the solvent (p < 0.05). The findings demonstrated that as the voltage gradient, process duration, temperature, and ethanol concentration in the solvent were increased, there was a significant initial increase followed by a decrease in both the total phenolic content (TPC) and the DPPH radical scavenging activity of FVE (p < 0.05). The optimization results indicated that the extraction process with an ethanol-to-water ratio of 65.5%, voltage gradient of 20.3 V/cm, temperature of 56.6°C, and extraction time of 22.1 min results in maximum extract TPC, DPPH, and EY, and minimum extraction EC. The OAE method showed greater EY and less EC and CO₂ production compared to the hydrodistillation (HD) method. In general, OAE caused improved antioxidant and antimicrobial properties of the FVE.