Structural characterization of a α-d-glucan from Ginkgo biloba seeds and its protective effects on non-alcoholic fatty liver disease in mice

IF 10.7 1区 化学 Q1 CHEMISTRY, APPLIED Carbohydrate Polymers Pub Date : 2024-11-18 DOI:10.1016/j.carbpol.2024.123022
Shuxiao Liang, Zhijie Yao, Jinxiang Chen, Jin Qian, Yufeng Dai, Haitao Li
{"title":"Structural characterization of a α-d-glucan from Ginkgo biloba seeds and its protective effects on non-alcoholic fatty liver disease in mice","authors":"Shuxiao Liang,&nbsp;Zhijie Yao,&nbsp;Jinxiang Chen,&nbsp;Jin Qian,&nbsp;Yufeng Dai,&nbsp;Haitao Li","doi":"10.1016/j.carbpol.2024.123022","DOIUrl":null,"url":null,"abstract":"<div><div>Nonalcoholic fatty liver disease (NAFLD) poses a great global challenge to public health, yet it holds promise for amelioration through plant-derived polysaccharide. <em>Ginkgo biloba</em> seeds have long been used as medicine and food, which has potential benefits for various chronic diseases. However, the protective role of <em>Ginkgo biloba</em> seed polysaccharide against NAFLD remains unclear. In this study, we isolated and purified polysaccharide (GBSP-2) from <em>Ginkgo biloba</em> seeds. GBSP-2 is composed of α-<span>d</span>-glucopyranose residues, which are interconnected with α-<span>d</span>-glucopyranose units linked by (1→4) bonds, (1→4,6) bonds and (1→3,4) bonds, the ratio distribution is 15:1:1. By studying a mouse model, we investigated the effect of GBSP-2 (100 or 200 mg/kg) on high-fat-diet-induced NAFLD. We demonstrated that GBSP-2 significantly alleviated NAFLD, as evidenced by reduced hepatic steatosis, decreased inflammation, improved oxidative stress and ameliorative glucolipid metabolic disorders. Furthermore, GBSP-2 mitigated gut microbiota disturbance of NAFLD mice and markedly increased the abundance of <em>Akkermansia</em>, <em>Romboutsia</em>, <em>Lactobacillus</em> and <em>Bacteroides</em>. Mechanistically, GBSP-2 could activate AMPK/ACC signaling pathway to inhibit lipid synthesis by generating 3,4-dihydroxyphenylpropionic acid (DHPPA). Overall, these findings suggest that GBSP-2 plays a multi-channel and multi-target role in improving NAFLD through the gut-liver axis.</div></div>","PeriodicalId":261,"journal":{"name":"Carbohydrate Polymers","volume":"349 ","pages":"Article 123022"},"PeriodicalIF":10.7000,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Carbohydrate Polymers","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0144861724012487","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0

Abstract

Nonalcoholic fatty liver disease (NAFLD) poses a great global challenge to public health, yet it holds promise for amelioration through plant-derived polysaccharide. Ginkgo biloba seeds have long been used as medicine and food, which has potential benefits for various chronic diseases. However, the protective role of Ginkgo biloba seed polysaccharide against NAFLD remains unclear. In this study, we isolated and purified polysaccharide (GBSP-2) from Ginkgo biloba seeds. GBSP-2 is composed of α-d-glucopyranose residues, which are interconnected with α-d-glucopyranose units linked by (1→4) bonds, (1→4,6) bonds and (1→3,4) bonds, the ratio distribution is 15:1:1. By studying a mouse model, we investigated the effect of GBSP-2 (100 or 200 mg/kg) on high-fat-diet-induced NAFLD. We demonstrated that GBSP-2 significantly alleviated NAFLD, as evidenced by reduced hepatic steatosis, decreased inflammation, improved oxidative stress and ameliorative glucolipid metabolic disorders. Furthermore, GBSP-2 mitigated gut microbiota disturbance of NAFLD mice and markedly increased the abundance of Akkermansia, Romboutsia, Lactobacillus and Bacteroides. Mechanistically, GBSP-2 could activate AMPK/ACC signaling pathway to inhibit lipid synthesis by generating 3,4-dihydroxyphenylpropionic acid (DHPPA). Overall, these findings suggest that GBSP-2 plays a multi-channel and multi-target role in improving NAFLD through the gut-liver axis.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
银杏叶籽中α-d-葡聚糖的结构特征及其对小鼠非酒精性脂肪肝的保护作用
非酒精性脂肪肝(NAFLD)是全球公共卫生面临的巨大挑战,但它有望通过植物多糖得到改善。银杏叶种子长期以来一直被用作药物和食品,对各种慢性疾病具有潜在的益处。然而,银杏叶多糖对非酒精性脂肪肝的保护作用仍不明确。在这项研究中,我们从银杏叶种子中分离纯化了多糖(GBSP-2)。GBSP-2由α-d-吡喃葡萄糖残基组成,α-d-吡喃葡萄糖单位之间以(1→4)键、(1→4,6)键和(1→3,4)键连接,比例分布为15:1:1。通过研究小鼠模型,我们考察了 GBSP-2(100 或 200 mg/kg)对高脂饮食诱发的非酒精性脂肪肝的影响。结果表明,GBSP-2 能显著缓解非酒精性脂肪肝,具体表现为减轻肝脏脂肪变性、减少炎症反应、改善氧化应激和糖脂代谢紊乱。此外,GBSP-2 还能缓解非酒精性脂肪肝小鼠肠道微生物群的紊乱,显著增加 Akkermansia、Romboutsia、Lactobacillus 和 Bacteroides 的丰度。从机制上讲,GBSP-2 可激活 AMPK/ACC 信号通路,通过生成 3,4-二羟基苯丙酸(DHPPA)来抑制脂质合成。总之,这些研究结果表明,GBSP-2 在通过肠肝轴改善非酒精性脂肪肝方面发挥着多渠道、多靶点的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Carbohydrate Polymers
Carbohydrate Polymers 化学-高分子科学
CiteScore
22.40
自引率
8.00%
发文量
1286
审稿时长
47 days
期刊介绍: Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.
期刊最新文献
Corrigendum to "Dissecting the Enterococcal Polysaccharide Antigen (EPA) structure to explore innate immune evasion and phage specificity" [Carbohydr. Polym. 347 (1 January 2025) 122686]. Editorial Board In situ growth of defective ZIF-8 on TEMPO-oxidized cellulose nanofibrils for rapid response release of curcumin in food preservation Polydopamine-integrated cellulose/graphene oxide monoliths: A versatile platform for efficient continuous-flow iodine capture and photothermal-enhanced reduction of Cr(VI) Accelerating repair of infected bone defects through post-reinforced injectable hydrogel mediated antibacterial/immunoregulatory microenvironment at bone-hydrogel interface
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1