Antibacterial betaine modified chitosan-based hydrogel with angiogenic property for photothermal enhanced diabetic wound repairing

Abstract

Chronic diabetes wound repairing is still a huge challenge in clinical practice. High concentration of reactive oxygen species and vascular disfunction are the main factors hindering the recovery of diabetes wounds. This research grafted betaine onto chitosan (CSBT) to enhance the antibacterial property and the CSBT was cross-linked with PEO₉₀ dialdehyde (PEO DA) to prepare hydrogel with Ca²⁺ loading to promote the coagulation. The polydopamine nanoparticles (PDA NPs) with photothermal property and antioxidant property was composited to the hydrogel and deferoxamine (DFO) was loaded to fabricate the multifunctional CBPCa/PDA/DFO hydrogel to promote vascular regeneration in combination with photothermal antibacterial performance for the diabetes wounds treatment. The CBPCa/PDA/DFO hydrogel showed good mechanical strength, injectability, anti-inflammatory property and coagulation performance. Furthermore, the antibacterial effect of chitosan based hydrogel was enhanced with near infrared (NIR) stimulated photothermal treatment. Combined with the photothermal effect and the angiogenic drug DFO release, the CBPCa/PDA/DFO hydrogel significantly enhanced vascular regeneration and reduced the inflammation in the in vivo wound repairing experiment. As a result, the CBPCa/PDA/DFO hydrogel may provide a promising therapeutic platform for diabetic trauma repairing.